##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632153.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	578885
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.839318690240724	34.0	31.0	34.0	31.0	34.0
2	33.020254454684434	34.0	33.0	34.0	31.0	34.0
3	33.102460765091514	34.0	33.0	34.0	31.0	34.0
4	36.482712455841835	37.0	37.0	37.0	35.0	37.0
5	36.34837143819584	37.0	37.0	37.0	35.0	37.0
6	36.37005795624347	37.0	37.0	37.0	35.0	37.0
7	36.34703956744431	37.0	37.0	37.0	35.0	37.0
8	36.33386251155238	37.0	37.0	37.0	35.0	37.0
9	38.15122347271047	39.0	39.0	39.0	37.0	39.0
10	38.12223153130587	39.0	38.0	39.0	37.0	39.0
11	38.18937612824654	39.0	39.0	39.0	37.0	39.0
12	38.152501792238525	39.0	38.0	39.0	37.0	39.0
13	38.17533361548494	39.0	39.0	39.0	37.0	39.0
14	39.59714796548537	41.0	39.0	41.0	37.0	41.0
15	39.61074824878862	41.0	39.0	41.0	37.0	41.0
16	39.606215396840476	41.0	39.0	41.0	37.0	41.0
17	39.59471743092324	41.0	39.0	41.0	37.0	41.0
18	39.585586083591735	41.0	39.0	41.0	37.0	41.0
19	39.61498397781943	41.0	39.0	41.0	37.0	41.0
20	39.58838111196524	41.0	39.0	41.0	37.0	41.0
21	39.56343660657989	41.0	39.0	41.0	37.0	41.0
22	39.5330678804944	41.0	39.0	41.0	37.0	41.0
23	39.49006279312817	40.0	39.0	41.0	37.0	41.0
24	39.45222971747411	41.0	39.0	41.0	37.0	41.0
25	39.39577981809859	40.0	39.0	41.0	37.0	41.0
26	39.32801160852328	40.0	39.0	41.0	36.0	41.0
27	39.25728253452758	40.0	39.0	41.0	36.0	41.0
28	39.20684246439276	40.0	39.0	41.0	36.0	41.0
29	39.154961693600626	40.0	39.0	41.0	36.0	41.0
30	39.0963697452862	40.0	39.0	41.0	36.0	41.0
31	39.034110401893294	40.0	39.0	41.0	35.0	41.0
32	38.94891213280703	40.0	38.0	41.0	35.0	41.0
33	38.88331879388825	40.0	38.0	41.0	35.0	41.0
34	38.81586498181849	40.0	38.0	41.0	35.0	41.0
35	38.804513849901106	40.0	38.0	41.0	35.0	41.0
36	38.771859695794504	40.0	38.0	41.0	35.0	41.0
37	38.73177574129577	40.0	38.0	41.0	35.0	41.0
38	38.68364701106437	40.0	38.0	41.0	35.0	41.0
39	38.64373752990663	40.0	38.0	41.0	35.0	41.0
40	38.58868168980022	40.0	38.0	41.0	35.0	41.0
41	38.55548165870596	40.0	38.0	41.0	35.0	41.0
42	38.51896317921521	40.0	38.0	41.0	35.0	41.0
43	37.64956597597105	39.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	5.0
18	9.0
19	17.0
20	15.0
21	49.0
22	87.0
23	176.0
24	251.0
25	417.0
26	623.0
27	839.0
28	1325.0
29	1954.0
30	2645.0
31	3849.0
32	5284.0
33	7851.0
34	12209.0
35	19161.0
36	31567.0
37	59223.0
38	147980.0
39	283347.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85289824403811	16.996640092591793	12.209678951777986	27.94078271159211
2	19.327673026594226	19.50456480993634	33.158226590773644	28.00953557269579
3	20.068407369339333	20.20021247743507	29.41931471708543	30.312065436140166
4	15.95360045604913	14.67908133739862	32.02017671903746	37.34714148751479
5	16.602088497715435	34.3004223636819	32.88857026870622	16.208918869896436
6	37.194088635912145	32.64016169014571	14.599790977482574	15.565958696459573
7	31.928448655605173	28.64679513202104	19.226616685524757	20.19813952684903
8	29.051711479827603	32.07493716368536	18.91774704820474	19.9556043082823
9	27.58008930962108	13.689420178446495	17.55149986612194	41.17899064581048
10	18.10895082788464	25.57278215880529	31.14815550584313	25.17011150746694
11	38.55670815446936	19.73207113675428	19.89894365893053	21.812277049845825
12	23.73960285721689	23.31793015883984	26.902579959750213	26.03988702419306
13	32.86974096754969	17.351805626333384	22.66063207718286	27.11782132893407
14	24.477746011729444	18.30225347003291	22.692417319502145	34.5275831987355
15	27.552795460238215	25.088057213436176	20.17550981628475	27.183637510040853
16	27.630531107214733	23.73597519369132	21.48906950430569	27.144424194788257
17	26.367240470905273	24.227091736700725	22.841669761697055	26.563998030696943
18	26.202095407550726	22.96224638745174	23.758777650137766	27.076880554859773
19	27.327534829888496	23.058984081466956	23.665322127883776	25.948158960760775
20	27.645041761317014	22.689826131269598	23.26904307418572	26.39608903322767
21	27.56091451670021	23.32138507648324	22.30926695284901	26.808433453967538
22	27.8613196057939	22.958445978043997	22.229112863522115	26.95112155263999
23	27.238570700570925	22.938925693358783	22.569594997279253	27.25290860879104
24	27.72657781770127	22.7635886229562	22.523471846739852	26.986361712602676
25	27.384022733358094	22.29527453639324	22.91318655691545	27.407516173333217
26	27.194865992381906	22.45333701857882	22.859462587560568	27.492334401478708
27	26.733116249341403	22.793819152335956	23.01597035680662	27.457094241516018
28	26.691657237620596	22.81333943702117	23.202363163668085	27.292640161690144
29	26.48367119548788	23.48117501749052	23.252804961261734	26.782348825759865
30	26.250982492204844	23.900774765281533	22.902994549867415	26.94524819264621
31	26.95544019969424	23.16401357782634	22.953781839225407	26.926764383254014
32	25.84071102205101	23.37666375877765	23.551828083298066	27.230797135873274
33	26.160463649947747	22.71314682536255	23.702980730196842	27.42340879449286
34	26.663672404709054	22.51621651968871	23.7827893277594	27.037321747842835
35	25.964051581920415	22.45212779740363	24.463235357627163	27.120585263048792
36	25.82395467148052	22.467156689152425	24.360451557735992	27.348437081631065
37	26.12764193233544	21.17398101522755	24.940704975945135	27.75767207649188
38	25.045043488775836	21.53536540072726	26.086355666496804	27.333235444000103
39	24.917384281852183	20.957184933104156	26.629814211803726	27.495616573239936
40	24.280642960173438	20.691674512208817	28.04563946206932	26.98204306554842
41	23.649429506724136	20.791694377985266	28.37748430171796	27.181391813572642
42	23.412767648151185	20.059597329348662	28.89917686587146	27.628458156628692
43	22.167788075351755	19.98877151765895	29.792791314337048	28.050649092652254
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	3.5
2	6.0
3	16.5
4	27.0
5	27.0
6	31.5
7	36.0
8	37.5
9	39.0
10	65.0
11	91.0
12	91.0
13	143.5
14	196.0
15	352.5
16	509.0
17	536.5
18	564.0
19	564.0
20	762.5
21	961.0
22	1260.5
23	1560.0
24	2054.5
25	2549.0
26	2549.0
27	3066.0
28	3583.0
29	4828.0
30	6073.0
31	7354.0
32	8635.0
33	8635.0
34	10282.0
35	11929.0
36	13835.5
37	15742.0
38	18551.5
39	21361.0
40	21361.0
41	24303.0
42	27245.0
43	28198.0
44	29151.0
45	32407.0
46	35663.0
47	35663.0
48	38043.5
49	40424.0
50	43959.0
51	47494.0
52	51611.5
53	55729.0
54	55729.0
55	52049.5
56	48370.0
57	47427.0
58	46484.0
59	44005.5
60	41527.0
61	41527.0
62	38866.5
63	36206.0
64	32908.0
65	29610.0
66	25942.0
67	22274.0
68	22274.0
69	19052.5
70	15831.0
71	13622.0
72	11413.0
73	9385.5
74	7358.0
75	7358.0
76	5825.5
77	4293.0
78	3576.0
79	2859.0
80	2210.0
81	1561.0
82	1561.0
83	1194.5
84	828.0
85	624.0
86	420.0
87	297.0
88	174.0
89	174.0
90	126.5
91	79.0
92	49.5
93	20.0
94	13.0
95	6.0
96	6.0
97	4.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	578885.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.8291921930849
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.62124465203851	64.37421606694758
2	6.457936829098405	9.277367713362214
3	1.6019677970494317	3.452041583441895
4	0.6647004443801459	1.9097958388084182
5	0.34725280172117956	1.2471444117208907
6	0.23203078094186125	0.9999950135390724
7	0.15963662002310197	0.8026598622485701
8	0.1227186506071068	0.705182523210713
9	0.08861136372987286	0.5728394408259952
>10	0.6006735900158433	8.326325684155526
>50	0.06472710956008426	3.188137458279357
>100	0.037296255808436686	4.614378846378069
>500	0.0012031050260751062	0.5299155570818183
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	756	0.13059588692054552	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	656	0.11332129870354214	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	591	0.10209281636248996	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.4549176434006755E-4	0.0
3	0.0	0.0	0.0	3.4549176434006755E-4	0.0
4	0.0	0.0	0.0	3.4549176434006755E-4	0.0
5	0.0	1.7274588217003377E-4	0.0	3.4549176434006755E-4	0.0
6	0.0	1.7274588217003377E-4	0.0	3.4549176434006755E-4	0.0
7	0.0	1.7274588217003377E-4	0.0	5.182376465101013E-4	0.0
8	0.0	1.7274588217003377E-4	0.0	5.182376465101013E-4	0.0
9	0.0	1.7274588217003377E-4	0.0	6.909835286801351E-4	0.0
10	0.0	1.7274588217003377E-4	0.0	0.0012092211751902364	0.0
11	0.0	1.7274588217003377E-4	0.0	0.0013819670573602702	0.0
12	0.0	1.7274588217003377E-4	0.0	0.0013819670573602702	0.0
13	0.0	1.7274588217003377E-4	0.0	0.0013819670573602702	0.0
14	0.0	1.7274588217003377E-4	0.0	0.001554712939530304	0.0
15	0.0	1.7274588217003377E-4	0.0	0.0017274588217003376	0.0
16	0.0	1.7274588217003377E-4	0.0	0.0019002047038703714	0.0
17	0.0	1.7274588217003377E-4	0.0	0.002245696468210439	0.0
18	0.0	1.7274588217003377E-4	0.0	0.002245696468210439	0.0
19	0.0	1.7274588217003377E-4	0.0	0.0027639341147205404	0.0
20	0.0	1.7274588217003377E-4	0.0	0.0027639341147205404	0.0
21	0.0	1.7274588217003377E-4	0.0	0.0027639341147205404	0.0
22	0.0	1.7274588217003377E-4	0.0	0.003109425879060608	0.0
23	0.0	1.7274588217003377E-4	0.0	0.003800409407740743	0.0
24	0.0	1.7274588217003377E-4	0.0	0.005182376465101013	0.0
25	0.0	1.7274588217003377E-4	0.0	0.005873359993781148	0.0
26	0.0	1.7274588217003377E-4	0.0	0.007428072933311452	0.0
27	0.0	1.7274588217003377E-4	0.0	0.028675816440225606	0.0
28	0.0	1.7274588217003377E-4	0.0	0.10986638106014147	0.0
29	0.0	1.7274588217003377E-4	0.0	0.24478091503493785	0.0
30	0.0	1.7274588217003377E-4	0.0	0.3999067172236282	0.0
31	0.0	3.4549176434006755E-4	0.0	0.9084705943322076	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGTT	20	0.0018411811	37.0	18
GGTATCA	190	0.0	28.236843	1
GTACTAT	35	8.864057E-4	26.42857	1
GTATATA	35	8.864057E-4	26.42857	1
GTATAGA	60	1.3354911E-6	24.666668	1
GTGTTAG	50	2.699858E-4	22.199999	1
TGTACCG	50	2.699858E-4	22.199999	5
CCTAAGC	80	6.94783E-7	20.8125	3
CGATTTG	45	0.0038235483	20.555555	28
GCATTAA	45	0.0038235483	20.555555	1
CTTATAT	45	0.0038235483	20.555555	1
GCGATTT	45	0.0038235483	20.555555	27
TAGGGTT	55	5.138855E-4	20.181818	5
TATAGAA	75	9.2527425E-6	19.733335	2
TCCTAAG	85	1.2431228E-6	19.588236	2
GCACCGT	70	1.2178713E-4	18.5	6
ATACCCA	60	9.2292664E-4	18.5	4
TATATAT	50	0.007030768	18.499998	2
TATACAG	50	0.007030768	18.499998	5
TAAGCCC	50	0.007030768	18.499998	4
>>END_MODULE