FastQCFastQC Report
Fri 10 Feb 2017
ERR1632152.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632152.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866729
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.024.7315791
TATACAG909.4862116E-820.5555555
CTTATAC23000.020.10869437
CTAGAAC4000.018.96253
CGAATTA801.6175522E-518.515
GTATCAA12350.018.42512
TCTAGAC3200.017.9218753
TAGAACT4100.017.597564
CGCGGAA852.7245907E-517.41176433
TATACAC6900.017.1594237
TCTAAGC1301.3946192E-817.0769233
TCGTATG650.001580433817.07692330
CTAGACA3850.016.8181844
CGCGCTT904.4483488E-516.44444512
GTCTAGA3600.016.4444451
GTTCTAG3800.016.065791
CTCGTAT700.002593306615.85714229
ATACACA4800.015.41666737
TATAGAG3500.015.3285713
GTCTATA855.366379E-415.2352941