##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632152.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1866729
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.633069395718394	34.0	31.0	34.0	31.0	34.0
2	32.80433206962553	34.0	31.0	34.0	31.0	34.0
3	32.893122676082065	34.0	31.0	34.0	31.0	34.0
4	36.328538850577665	37.0	37.0	37.0	35.0	37.0
5	36.181646077175635	37.0	35.0	37.0	35.0	37.0
6	36.20601383489516	37.0	36.0	37.0	35.0	37.0
7	36.181177342828015	37.0	36.0	37.0	35.0	37.0
8	36.165252696026045	37.0	36.0	37.0	35.0	37.0
9	37.94466845482124	39.0	38.0	39.0	35.0	39.0
10	37.89083257398369	39.0	38.0	39.0	35.0	39.0
11	37.96941280710805	39.0	38.0	39.0	35.0	39.0
12	37.93116462003858	39.0	38.0	39.0	35.0	39.0
13	37.954500090800536	39.0	38.0	39.0	35.0	39.0
14	39.31956593592321	40.0	39.0	41.0	36.0	41.0
15	39.317650821302934	40.0	39.0	41.0	36.0	41.0
16	39.32697033152643	40.0	39.0	41.0	36.0	41.0
17	39.30834148931098	40.0	39.0	41.0	36.0	41.0
18	39.30911824908704	40.0	39.0	41.0	36.0	41.0
19	39.3258737610012	40.0	39.0	41.0	36.0	41.0
20	39.29418464062004	40.0	39.0	41.0	36.0	41.0
21	39.260727722127854	40.0	39.0	41.0	36.0	41.0
22	39.23019195609004	40.0	39.0	41.0	36.0	41.0
23	39.18579504577258	40.0	39.0	41.0	36.0	41.0
24	39.13656454686245	40.0	39.0	41.0	36.0	41.0
25	39.08321668544283	40.0	39.0	41.0	36.0	41.0
26	39.00816347739816	40.0	39.0	41.0	36.0	41.0
27	38.92986662766797	40.0	38.0	41.0	35.0	41.0
28	38.86470076802792	40.0	38.0	41.0	35.0	41.0
29	38.81344640812887	40.0	38.0	41.0	35.0	41.0
30	38.7474384337523	40.0	38.0	41.0	35.0	41.0
31	38.67958927085828	40.0	38.0	41.0	35.0	41.0
32	38.59587224498039	40.0	38.0	41.0	35.0	41.0
33	38.521990604956585	40.0	38.0	41.0	35.0	41.0
34	38.449318031701445	40.0	38.0	41.0	34.0	41.0
35	38.43413264592772	40.0	38.0	41.0	34.0	41.0
36	38.39699120761503	40.0	38.0	41.0	34.0	41.0
37	38.35638702779032	40.0	38.0	41.0	34.0	41.0
38	38.316534430011	40.0	38.0	41.0	34.0	41.0
39	38.2746269008517	40.0	38.0	41.0	34.0	41.0
40	38.212923247027284	40.0	38.0	41.0	34.0	41.0
41	38.171224639462935	40.0	38.0	41.0	34.0	41.0
42	38.12606275469015	40.0	37.0	41.0	34.0	41.0
43	37.23667066831875	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	1.0
14	1.0
15	3.0
16	6.0
17	11.0
18	24.0
19	52.0
20	117.0
21	187.0
22	398.0
23	715.0
24	1241.0
25	1976.0
26	3032.0
27	4464.0
28	6550.0
29	9565.0
30	13550.0
31	18350.0
32	25055.0
33	34330.0
34	49903.0
35	73656.0
36	117387.0
37	212594.0
38	512984.0
39	780574.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63552502800353	16.673068238614174	11.366566866427853	28.32483986695444
2	19.66032562841205	19.25282137900038	32.810172231748695	28.27668076083888
3	20.512297178647785	19.730234008257224	28.656114519033025	31.101354294061966
4	15.658352122884466	14.160544996086738	32.01937721008245	38.161725670946346
5	17.14876663939972	33.612109738478374	32.39184691511194	16.84727670700996
6	37.97551760325146	32.16514019978261	14.506069172332994	15.353273024632927
7	32.675391018192784	28.179719712931018	19.026382511869695	20.118506757006507
8	29.259737219489278	31.24754584087996	18.838567354982967	20.6541495846478
9	28.093097605490673	13.156757086861564	17.787852441355977	40.96229286629179
10	17.943097257288017	25.394687713106723	31.113568171919976	25.548646857685288
11	39.129514782274235	19.473635433959615	19.654218689483045	21.7426310942831
12	24.547698139365703	22.969375844056636	26.90524441415974	25.57768160241792
13	32.76849505204022	17.426364512470744	22.573121218987865	27.232019216501165
14	24.78399382020636	18.14109064572308	22.57446045998107	34.500455074089494
15	27.415977359327464	25.175855734817425	20.248413133347153	27.159753772507955
16	27.63041662715906	23.36745183687616	21.569226170483237	27.432905365481545
17	26.23417753728581	24.04719699538605	22.999910538701656	26.71871492862649
18	26.77967717863707	22.620530350147234	23.715118798711543	26.88467367250415
19	27.21910893332669	22.874878999576264	23.22661725403098	26.679394813066065
20	27.304606078332743	23.064836942052114	23.288275909358028	26.34228107025712
21	28.075312485100945	22.91510979901207	22.976286327581562	26.033291388305425
22	28.27716288759643	22.723330488785464	22.10910099966305	26.890405623955054
23	27.19993100230403	22.79404241322656	22.692581515581534	27.313445068887876
24	27.06568548514541	22.644261700546785	22.635422709991648	27.65463010431616
25	27.361764883922625	22.39950201662909	22.99316076409591	27.245572335352374
26	27.27696414423304	22.338271918419867	22.82168434732626	27.563079590020834
27	27.088988278427127	22.596852569387416	23.163565788071004	27.15059336411445
28	26.627110844691437	22.722366235270357	23.222224543573276	27.428298376464927
29	26.67634134360156	22.981161164796816	22.961233258817966	27.381264232783654
30	26.097146398861327	23.223992341684305	23.67403088504009	27.004830374414286
31	27.106076993500395	23.0253561175725	22.68551032313742	27.183056565789677
32	26.169197564295622	22.628726505025636	23.536624759137506	27.66545117154124
33	26.218963759603024	22.37030656297727	23.842507401984967	27.568222275434728
34	26.8367288449475	21.772522953251382	24.004823410361116	27.385924791440004
35	26.179697213682328	21.97126631664264	24.593178763494862	27.25585770618017
36	26.046844507156635	21.425552396732467	25.049859942176933	27.477743153933964
37	25.996649754731404	20.98628135096203	25.131767921321195	27.885300972985366
38	25.0989833018076	20.670327615845686	26.563577251973907	27.66711183037281
39	25.036360392965452	20.384051461138707	27.1739497270359	27.40563841885994
40	24.6685512465923	20.878070678711264	27.29640992345434	27.156968151242094
41	24.24127980012096	19.96342264999365	28.71927312427246	27.076024425612932
42	23.496608238260617	20.231217278994436	28.864982544332896	27.407191938412055
43	22.23600747617892	19.80464223783956	29.33714534889639	28.622204937085137
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	29.0
2	37.0
3	83.5
4	130.0
5	130.0
6	159.5
7	189.0
8	215.5
9	242.0
10	318.5
11	395.0
12	395.0
13	659.0
14	923.0
15	1567.5
16	2212.0
17	2300.5
18	2389.0
19	2389.0
20	2935.5
21	3482.0
22	3845.0
23	4208.0
24	5346.5
25	6485.0
26	6485.0
27	8220.5
28	9956.0
29	12609.5
30	15263.0
31	18771.5
32	22280.0
33	22280.0
34	28410.5
35	34541.0
36	41540.0
37	48539.0
38	55474.0
39	62409.0
40	62409.0
41	70330.0
42	78251.0
43	87273.0
44	96295.0
45	108649.5
46	121004.0
47	121004.0
48	128703.5
49	136403.0
50	144244.5
51	152086.0
52	156194.5
53	160303.0
54	160303.0
55	161330.5
56	162358.0
57	158276.5
58	154195.0
59	152382.5
60	150570.0
61	150570.0
62	140478.5
63	130387.0
64	114952.5
65	99518.0
66	88876.0
67	78234.0
68	78234.0
69	64684.5
70	51135.0
71	43101.0
72	35067.0
73	27085.0
74	19103.0
75	19103.0
76	15296.5
77	11490.0
78	9136.5
79	6783.0
80	5792.5
81	4802.0
82	4802.0
83	3599.5
84	2397.0
85	1927.0
86	1457.0
87	1101.5
88	746.0
89	746.0
90	554.5
91	363.0
92	206.0
93	49.0
94	35.5
95	22.0
96	22.0
97	15.5
98	9.0
99	5.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1866729.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.82839053389925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.1892265433009	45.31770565119718
2	8.304221207420193	8.94005724465805
3	2.571362023688058	4.152368376453549
4	1.2557727564105223	2.7038490541558695
5	0.7401305543767354	1.9920018263531132
6	0.5208974393439791	1.6823442475869483
7	0.3532656249358621	1.3311004014874694
8	0.2632498248472079	1.1336251503884864
9	0.21053831639366605	1.0199644845469111
>10	1.3273571449560004	13.830184445781779
>50	0.1526293608872804	5.700781108936974
>100	0.10635639951149349	10.39466342845865
>500	0.004693235692516549	1.6040887038101581
>1k	2.9956823569254567E-4	0.19726587618480984
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.3569639728101935E-5	0.0	0.0	0.0
3	0.0	5.3569639728101935E-5	0.0	0.0	0.0
4	0.0	5.3569639728101935E-5	0.0	0.0	0.0
5	0.0	5.3569639728101935E-5	0.0	0.0	0.0
6	0.0	5.3569639728101935E-5	0.0	0.0	0.0
7	0.0	5.3569639728101935E-5	0.0	0.0	0.0
8	0.0	5.3569639728101935E-5	0.0	0.0	0.0
9	0.0	5.3569639728101935E-5	0.0	5.3569639728101935E-5	0.0
10	0.0	5.3569639728101935E-5	0.0	2.1427855891240774E-4	0.0
11	0.0	5.3569639728101935E-5	0.0	2.678481986405097E-4	0.0
12	0.0	5.3569639728101935E-5	0.0	4.285571178248155E-4	0.0
13	0.0	5.3569639728101935E-5	0.0	4.821267575529174E-4	0.0
14	0.0	1.0713927945620387E-4	0.0	4.821267575529174E-4	0.0
15	0.0	1.0713927945620387E-4	0.0	5.356963972810194E-4	0.0
16	0.0	1.607089191843058E-4	0.0	6.428356767372232E-4	0.0
17	0.0	1.607089191843058E-4	0.0	8.03544595921529E-4	0.0
18	0.0	1.607089191843058E-4	0.0	9.642535151058348E-4	0.0
19	0.0	1.607089191843058E-4	0.0	0.0011785320740182426	0.0
20	0.0	1.607089191843058E-4	0.0	0.0013928106329306504	0.0
21	0.0	1.607089191843058E-4	0.0	0.00166065883157116	0.0
22	0.0	1.607089191843058E-4	0.0	0.002410633787764587	0.0
23	0.0	1.607089191843058E-4	0.0	0.003267748023414218	0.0
24	0.0	2.678481986405097E-4	0.0	0.0039641533398795436	0.0
25	0.0	2.678481986405097E-4	0.0	0.004874837215257276	0.0
26	0.0	2.678481986405097E-4	0.0	0.008142585238671495	0.0
27	0.0	3.214178383686116E-4	0.0	0.03240963203550167	0.0
28	0.0	4.285571178248155E-4	0.0	0.13344197256270193	0.0
29	0.0	6.428356767372232E-4	0.0	0.28381195127948405	0.0
30	0.0	6.428356767372232E-4	0.0	0.4841624038625853	0.0
31	0.0	6.428356767372232E-4	0.0	1.1518008237939197	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	950	0.0	24.731579	1
TATACAG	90	9.4862116E-8	20.555555	5
CTTATAC	2300	0.0	20.108694	37
CTAGAAC	400	0.0	18.9625	3
CGAATTA	80	1.6175522E-5	18.5	15
GTATCAA	1235	0.0	18.4251	2
TCTAGAC	320	0.0	17.921875	3
TAGAACT	410	0.0	17.59756	4
CGCGGAA	85	2.7245907E-5	17.411764	33
TATACAC	690	0.0	17.15942	37
TCTAAGC	130	1.3946192E-8	17.076923	3
TCGTATG	65	0.0015804338	17.076923	30
CTAGACA	385	0.0	16.818184	4
CGCGCTT	90	4.4483488E-5	16.444445	12
GTCTAGA	360	0.0	16.444445	1
GTTCTAG	380	0.0	16.06579	1
CTCGTAT	70	0.0025933066	15.857142	29
ATACACA	480	0.0	15.416667	37
TATAGAG	350	0.0	15.328571	3
GTCTATA	85	5.366379E-4	15.235294	1
>>END_MODULE