FastQCFastQC Report
Fri 10 Feb 2017
ERR1632151.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632151.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences703218
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14410.20491511878251126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10900.15500172066130274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10120.14390985441214532No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC9310.13239137792263567No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT8330.11845544340446348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5550.028.01
GTAATAC502.7006576E-422.1999993
TTAGACG450.003824354420.5555534
ATCGTGT450.003824354420.5555538
TTAGACT555.140366E-420.1818184
AATAGAC656.897262E-519.9230772
ATTAGAC609.231957E-418.53
CGGTGTA500.00703223418.4999989
GTATCAA8650.017.7514442
ATATAAC1156.394475E-817.6956523
GTACGGG1054.790709E-717.61904737
AAGACGG852.720682E-517.4117645
TTAGGCT752.0658289E-417.2666664
AAGACCG650.001579109717.0769235
CACGATT650.001579109717.07692325
TTTAGAC1005.8712667E-616.653
GTGTAGA904.4420103E-516.4444431
GCTTTAT2050.016.2439041
CGTTTAG803.3802286E-416.187526
TAACACT1151.2404416E-616.0869564
Produced by FastQC (version 0.11.5)