FastQCFastQC Report
Fri 10 Feb 2017
ERR1632150.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632150.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85946
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT1330.15474833034696203TruSeq Adapter, Index 8 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA352.3628654E-531.7142851
TCACAAC250.005468426329.615
GCAGACG250.005468426329.63
GTATCAA405.8773574E-527.752
TGGAGTG400.001916756523.12522
AGAACAG502.6740052E-422.25
CTTATAC1107.094059E-1121.86363637
CCAGCTA450.003797492720.55555510
AGGGCAT500.006983311418.531
AGAGAAA500.006983311418.515
ATCAACG852.675947E-517.4117642
TATCAAC904.369434E-516.4444451
CAACGCA904.369434E-516.4444454
TCAACGC904.369434E-516.4444453
CCCAGCT750.00406344614.89
TCTTATA1651.5752448E-814.57575736
TCTCTTA4050.013.70370437
AACGCAG1102.4151463E-413.4545455
ACGCAGA1153.517534E-412.8695656
AGAGTAC1205.036627E-412.33333410
Produced by FastQC (version 0.11.5)