##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632150.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85946
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17099108742699	33.0	31.0	34.0	30.0	34.0
2	32.35886486863845	34.0	31.0	34.0	31.0	34.0
3	32.43811230307402	34.0	31.0	34.0	30.0	34.0
4	36.03334651990785	37.0	35.0	37.0	35.0	37.0
5	35.748760849835946	37.0	35.0	37.0	35.0	37.0
6	35.751355502292135	37.0	35.0	37.0	35.0	37.0
7	35.72601400879622	37.0	35.0	37.0	33.0	37.0
8	35.70765364298513	37.0	35.0	37.0	33.0	37.0
9	37.43771670583855	39.0	37.0	39.0	35.0	39.0
10	37.32006143392363	39.0	37.0	39.0	34.0	39.0
11	37.405359179019385	39.0	37.0	39.0	35.0	39.0
12	37.34249412421753	39.0	37.0	39.0	34.0	39.0
13	37.39301421823005	39.0	37.0	39.0	35.0	39.0
14	38.6318036906895	40.0	38.0	41.0	35.0	41.0
15	38.61054615688921	40.0	38.0	41.0	35.0	41.0
16	38.60394899122705	40.0	38.0	41.0	35.0	41.0
17	38.579061271030646	40.0	38.0	41.0	35.0	41.0
18	38.56817071184232	40.0	38.0	41.0	35.0	41.0
19	38.59219742629093	40.0	38.0	41.0	34.0	41.0
20	38.533009098736414	40.0	38.0	41.0	34.0	41.0
21	38.51096037046518	40.0	38.0	41.0	34.0	41.0
22	38.460963861029015	40.0	38.0	41.0	34.0	41.0
23	38.3949689339818	40.0	38.0	41.0	34.0	41.0
24	38.34039978591208	40.0	38.0	41.0	34.0	41.0
25	38.27269448258209	40.0	38.0	41.0	34.0	41.0
26	38.16545272613036	40.0	38.0	41.0	34.0	41.0
27	38.0733483815419	40.0	38.0	41.0	34.0	41.0
28	37.98728271240081	40.0	38.0	41.0	33.0	41.0
29	37.90922206967166	40.0	37.0	41.0	33.0	41.0
30	37.81352244432551	40.0	37.0	41.0	33.0	41.0
31	37.751227514951246	40.0	37.0	41.0	33.0	41.0
32	37.646487329253254	40.0	37.0	41.0	33.0	41.0
33	37.5558490214786	39.0	37.0	41.0	32.0	41.0
34	37.50682986991832	39.0	37.0	41.0	32.0	41.0
35	37.46326763316501	39.0	37.0	41.0	32.0	41.0
36	37.42320759546692	39.0	37.0	40.0	32.0	41.0
37	37.38063435180229	39.0	36.0	40.0	32.0	41.0
38	37.32223721871873	39.0	36.0	40.0	32.0	41.0
39	37.30113094268494	39.0	36.0	40.0	32.0	41.0
40	37.246398901635914	39.0	36.0	40.0	32.0	41.0
41	37.20913131501175	39.0	36.0	40.0	32.0	41.0
42	37.1908291252647	39.0	36.0	40.0	32.0	41.0
43	36.232064319456406	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	1.0
18	2.0
19	19.0
20	11.0
21	26.0
22	49.0
23	75.0
24	104.0
25	191.0
26	248.0
27	340.0
28	497.0
29	728.0
30	1029.0
31	1350.0
32	1849.0
33	2614.0
34	3552.0
35	5195.0
36	8204.0
37	14377.0
38	26674.0
39	18809.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.371954483047496	17.733227840737207	12.86389128057152	27.030926395643778
2	20.512880180578502	19.959044050915693	32.35869034044633	27.169385428059478
3	21.066716310241315	20.518697786982525	28.82973029576711	29.584855607009054
4	16.561561910967352	15.251436948781794	31.244036953435877	36.942964186814976
5	16.923417029297465	34.27151932608847	32.76126870360459	16.04379494100947
6	37.78535359411724	32.288879063598074	14.473041211923764	15.452726130360924
7	31.587275731273124	28.42249784748563	19.461056942731485	20.52916947850976
8	28.683126614385777	31.595420380238753	19.125962813859864	20.5954901915156
9	27.66039140855886	13.614362506690247	17.58895120191748	41.136294882833404
10	18.235869034044633	25.446210411188417	31.12070369767063	25.197216857096315
11	38.54280594792079	20.050962232099227	19.459893421450676	21.946338398529306
12	23.68696623461243	23.526400297861446	26.623693947362298	26.162939520163825
13	32.84969632094571	17.401624275708002	22.283759569962534	27.464919833383757
14	25.062248388523027	18.836246014939615	22.25234449538082	33.84916110115654
15	27.589416610429808	25.58699648616573	19.61696879435925	27.206618109045216
16	27.645265631908405	23.700928489982083	21.507690875666118	27.146115002443395
17	26.446838712680055	24.244292928117655	22.67121215647034	26.637656202731947
18	26.01168175365928	22.594419751937263	24.154701789495732	27.23919670490773
19	27.43350475880204	23.043538966327695	23.303004211947037	26.21995206292323
20	27.900076792404533	22.471086496172017	22.98768994484909	26.64114676657436
21	28.047843995066668	23.156400530565705	21.949828962371722	26.845926511995906
22	28.024573569450585	22.95162078514416	21.82300514276406	27.200800502641194
23	26.684197053964116	23.180834477462593	22.47574058129523	27.65922788727806
24	27.35903939683057	22.835268657063736	22.480394666418448	27.325297279687245
25	27.357875875549766	22.384985921392502	22.588602145533244	27.66853605752449
26	27.107718800176855	22.964419519233008	22.652595815977474	27.275265864612663
27	27.141460917320178	22.659576943662298	22.536243687897052	27.662718451120472
28	26.700486351895375	22.86901077420706	23.16338165825053	27.267121215647034
29	26.122216275335674	23.179670956181788	23.131966583668813	27.56614618481372
30	26.217625020361623	23.998789937867965	22.991180508691503	26.792404533078912
31	27.112372885300072	23.24133758406441	22.680520326716774	26.96576920391874
32	25.860423987154725	23.06448234938217	23.377469573918507	27.697624089544597
33	25.689386358876504	22.696809624648033	23.972028948409466	27.641775068065993
34	26.59344239406139	22.579293975286806	23.821934703185722	27.00532892746608
35	26.185046424499102	22.001023898727105	24.655015940241547	27.15891373653224
36	25.438065762222788	22.41989155981663	24.58985874851651	27.55218392944407
37	25.865078072277942	20.96898052265376	25.543946198775973	27.621995206292322
38	24.730644823493822	21.247062108765967	26.71561212854583	27.30668093919438
39	24.37809787541014	20.189421264514927	27.590580131710606	27.841900728364323
40	23.660205245153936	20.213855211411815	28.82507621064389	27.30086333279036
41	22.71775300770251	20.51986130826333	29.561585181392967	27.200800502641194
42	22.756149209969053	19.90901263584111	29.599981383659507	27.73485677053033
43	21.292439438717334	20.006748423428665	30.903125218160238	27.79768691969376
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	1.5
4	1.0
5	1.0
6	1.5
7	2.0
8	2.5
9	3.0
10	5.0
11	7.0
12	7.0
13	10.5
14	14.0
15	37.0
16	60.0
17	54.5
18	49.0
19	49.0
20	72.5
21	96.0
22	123.0
23	150.0
24	193.0
25	236.0
26	236.0
27	330.0
28	424.0
29	626.5
30	829.0
31	991.5
32	1154.0
33	1154.0
34	1382.0
35	1610.0
36	1948.5
37	2287.0
38	2757.5
39	3228.0
40	3228.0
41	3599.0
42	3970.0
43	4241.0
44	4512.0
45	4960.0
46	5408.0
47	5408.0
48	5783.5
49	6159.0
50	6841.0
51	7523.0
52	8063.0
53	8603.0
54	8603.0
55	8113.0
56	7623.0
57	7364.0
58	7105.0
59	6601.5
60	6098.0
61	6098.0
62	5825.0
63	5552.0
64	4909.5
65	4267.0
66	3653.0
67	3039.0
68	3039.0
69	2608.0
70	2177.0
71	1868.5
72	1560.0
73	1237.0
74	914.0
75	914.0
76	730.5
77	547.0
78	437.5
79	328.0
80	271.5
81	215.0
82	215.0
83	164.5
84	114.0
85	78.5
86	43.0
87	32.0
88	21.0
89	21.0
90	15.0
91	9.0
92	6.5
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	85946.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.81004351569591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.67562236332483	74.8912107602448
2	3.85584495990094	6.231819979987434
3	1.190733301657236	2.8866962976752846
4	0.6061653204325227	1.9593698368743164
5	0.3945113961959887	1.5940241547017893
6	0.2361309086720516	1.1449049403113583
7	0.19293623025643242	1.091382961394364
8	0.13822297092998143	0.8935843436576454
9	0.10366722819748608	0.7539617899611384
>10	0.5989662073632529	8.113233891047868
>50	0.005759290455415893	0.28506271379703535
>100	0.0014398226138539732	0.15474833034696203
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	133	0.15474833034696203	TruSeq Adapter, Index 8 (95% over 22bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0011635212808042258	0.0
17	0.0	0.0	0.0	0.0011635212808042258	0.0
18	0.0	0.0	0.0	0.0011635212808042258	0.0
19	0.0	0.0	0.0	0.0011635212808042258	0.0
20	0.0	0.0	0.0	0.0023270425616084517	0.0
21	0.0	0.0	0.0	0.0023270425616084517	0.0
22	0.0	0.0	0.0	0.0023270425616084517	0.0
23	0.0	0.0	0.0	0.0023270425616084517	0.0
24	0.0	0.0	0.0	0.0034905638424126777	0.0
25	0.0	0.0	0.0	0.0034905638424126777	0.0
26	0.0	0.0	0.0	0.005817606404021129	0.0
27	0.0	0.0	0.0	0.03374211714332255	0.0
28	0.0	0.0	0.0	0.12449677704605218	0.0
29	0.0	0.0	0.0	0.2699369371465804	0.0
30	0.0	0.0	0.0	0.4444651292672143	0.0
31	0.0	0.0	0.0	0.9284899820817722	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	35	2.3628654E-5	31.714285	1
TCACAAC	25	0.0054684263	29.6	15
GCAGACG	25	0.0054684263	29.6	3
GTATCAA	40	5.8773574E-5	27.75	2
TGGAGTG	40	0.0019167565	23.125	22
AGAACAG	50	2.6740052E-4	22.2	5
CTTATAC	110	7.094059E-11	21.863636	37
CCAGCTA	45	0.0037974927	20.555555	10
AGGGCAT	50	0.0069833114	18.5	31
AGAGAAA	50	0.0069833114	18.5	15
ATCAACG	85	2.675947E-5	17.411764	2
TATCAAC	90	4.369434E-5	16.444445	1
CAACGCA	90	4.369434E-5	16.444445	4
TCAACGC	90	4.369434E-5	16.444445	3
CCCAGCT	75	0.004063446	14.8	9
TCTTATA	165	1.5752448E-8	14.575757	36
TCTCTTA	405	0.0	13.703704	37
AACGCAG	110	2.4151463E-4	13.454545	5
ACGCAGA	115	3.517534E-4	12.869565	6
AGAGTAC	120	5.036627E-4	12.333334	10
>>END_MODULE