FastQCFastQC Report
Fri 10 Feb 2017
ERR1632149.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632149.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507249
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGT303.596668E-430.83333435
TGCGTTA303.596668E-430.83333437
GTAATAC405.9336875E-527.753
GGTATCA1950.027.5128211
ATACTGA358.862534E-426.4285726
CGTGTTG400.001929707923.12524
ATGCGTT400.001929707923.12536
GTTATAC400.001929707923.1251
CGGACAT1107.4578566E-1121.86363621
AGAGGTC1500.020.9666658
GTCGGGA656.892415E-519.9230772
CTTATAC5600.019.49107237
TCGCCAT1551.8189894E-1219.09677513
GTATCAA2950.018.8135592
TCTTACC609.2271186E-418.52
GGATACC500.00702959518.525
GTGTGCG609.2271186E-418.59
GCGGGTA609.2271186E-418.523
GACGGAC500.00702959518.57
ATTTGCG801.6137703E-518.516
Produced by FastQC (version 0.11.5)