##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632149.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	507249
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79549097188954	34.0	31.0	34.0	31.0	34.0
2	32.96775942387269	34.0	31.0	34.0	31.0	34.0
3	33.05140079132734	34.0	33.0	34.0	31.0	34.0
4	36.44108120469434	37.0	37.0	37.0	35.0	37.0
5	36.30960337033686	37.0	37.0	37.0	35.0	37.0
6	36.338758676705126	37.0	37.0	37.0	35.0	37.0
7	36.31686607563544	37.0	37.0	37.0	35.0	37.0
8	36.29950379399467	37.0	37.0	37.0	35.0	37.0
9	38.099718284314015	39.0	39.0	39.0	37.0	39.0
10	38.069084414163456	39.0	38.0	39.0	35.0	39.0
11	38.14115552716713	39.0	38.0	39.0	37.0	39.0
12	38.10868232367141	39.0	38.0	39.0	37.0	39.0
13	38.122740508113374	39.0	38.0	39.0	37.0	39.0
14	39.53704393700136	41.0	39.0	41.0	37.0	41.0
15	39.556513664886474	41.0	39.0	41.0	37.0	41.0
16	39.54550526467278	41.0	39.0	41.0	37.0	41.0
17	39.53629676943671	41.0	39.0	41.0	37.0	41.0
18	39.52918980618986	41.0	39.0	41.0	37.0	41.0
19	39.5616531525937	41.0	39.0	41.0	37.0	41.0
20	39.54763045368251	41.0	39.0	41.0	37.0	41.0
21	39.51820703441505	41.0	39.0	41.0	37.0	41.0
22	39.480978769795506	41.0	39.0	41.0	37.0	41.0
23	39.4353010060148	40.0	39.0	41.0	37.0	41.0
24	39.39708308937031	41.0	39.0	41.0	37.0	41.0
25	39.34045803934557	40.0	39.0	41.0	36.0	41.0
26	39.27494386386173	40.0	39.0	41.0	36.0	41.0
27	39.19910931317755	40.0	39.0	41.0	36.0	41.0
28	39.13854734065518	40.0	39.0	41.0	36.0	41.0
29	39.09104404345795	40.0	39.0	41.0	36.0	41.0
30	39.02246036956209	40.0	39.0	41.0	35.0	41.0
31	38.964445469582	40.0	39.0	41.0	35.0	41.0
32	38.89386080603412	40.0	38.0	41.0	35.0	41.0
33	38.83024116360998	40.0	38.0	41.0	35.0	41.0
34	38.770495358295435	40.0	38.0	41.0	35.0	41.0
35	38.75042631922389	40.0	38.0	41.0	35.0	41.0
36	38.72925328586158	40.0	38.0	41.0	35.0	41.0
37	38.69409106770048	40.0	38.0	41.0	35.0	41.0
38	38.6547454997447	40.0	38.0	41.0	35.0	41.0
39	38.61285483066502	40.0	38.0	41.0	35.0	41.0
40	38.55966990570706	40.0	38.0	41.0	35.0	41.0
41	38.53580391484261	40.0	38.0	41.0	35.0	41.0
42	38.506960092577806	40.0	38.0	41.0	35.0	41.0
43	37.6661462122153	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	1.0
17	3.0
18	9.0
19	15.0
20	36.0
21	62.0
22	104.0
23	166.0
24	291.0
25	395.0
26	615.0
27	885.0
28	1303.0
29	1927.0
30	2746.0
31	3680.0
32	5219.0
33	7598.0
34	11264.0
35	16931.0
36	27567.0
37	50556.0
38	128071.0
39	247803.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.36814661044181	17.99195267018762	12.675234450930411	26.96466626844016
2	19.026158750436174	20.416600131296462	33.97522715668242	26.582013961584945
3	19.944248288315993	21.254452941257647	30.165658286167147	28.635640484259213
4	15.70195308418548	15.530242543602846	33.232988137975624	35.53481623423605
5	16.117133794250947	34.8302313065181	33.356004644661695	15.696630254569255
6	35.56438750988173	33.68917435026979	15.39776322871016	15.348674911138316
7	30.296166182683454	29.29586849850862	20.394125961805738	20.013839357002183
8	27.510749158697205	32.50908331016917	20.186535606773003	19.793631924360618
9	26.95579488574645	14.217475046771902	19.09496125177181	39.73176881570984
10	17.5245293731481	26.115970657408887	32.48877770089246	23.870722268550555
11	36.628756291288894	20.816403778026178	20.785255367679383	21.769584563005544
12	22.726905326575313	24.57037865032755	27.92297274119811	24.779743281899027
13	31.659796273624984	18.634635060887256	23.480972855540376	26.22459580994738
14	23.97087032207062	20.03197640606487	23.554112477304045	32.44304079456046
15	26.328489558382568	26.439874696647998	21.209307460438563	26.02232828453087
16	26.140810528951263	25.27614642907132	22.627545840405798	25.955497201571614
17	24.936668184658817	25.313406236384893	24.214537633391096	25.535387945565198
18	24.70719508564827	24.097041098158893	25.270429315779825	25.925334500413012
19	25.750272548590537	24.366928273885215	24.958748070474265	24.924051107049987
20	26.48994872340803	23.8226196601669	24.610989868880964	25.076441747544102
21	26.385857833135205	24.43730790992195	23.516458386315204	25.66037587062764
22	26.734010318403783	24.08954970832865	23.658794793089786	25.517645180177784
23	25.87604904100353	24.1745178403506	23.79797693046216	26.151456188183715
24	26.244901419224085	24.23208325694087	23.963970357753293	25.559044966081746
25	26.18063318015413	23.63730633278725	24.031984291738375	26.15007619532025
26	25.80034657535057	24.02528146925869	24.117149565597963	26.057222389792784
27	25.6814700472549	23.95095899646919	24.138638025900494	26.228932930375414
28	25.34810319980917	24.154015089236253	24.48422766727978	26.013654043674805
29	25.037801947367072	24.584572862637483	24.605864181102376	25.77176100889307
30	25.12710719981705	25.138147142724776	24.328682757383454	25.406062900074716
31	25.685807167682935	24.461359214113777	24.062738418409893	25.790095199793395
32	24.538441672630206	24.3549026217893	24.840660109729146	26.26599559585135
33	24.8071459973307	23.837208156152105	25.213258182864827	26.142387663652368
34	25.32129190989041	23.474664316735964	25.226663827824204	25.977379945549423
35	25.234746643167362	23.31714798846326	25.55786211505592	25.89024325331346
36	24.51912177254169	23.31990797419019	25.937163010671288	26.22380724259683
37	24.870428527212475	22.17609103221495	26.160918996390333	26.792561444182244
38	23.834645312262815	22.33084737476072	27.55983747626905	26.274669836707414
39	23.631392077658113	21.738041868983476	28.29064226839284	26.33992378496557
40	22.98910397063375	21.457903317699987	29.43978203998431	26.11321067168195
41	22.350955842199788	21.586045512164638	29.94308515147393	26.119913494161644
42	22.220053662008205	21.283235649552783	30.083647281709773	26.413063406729236
43	21.094965194608566	21.271407139294507	30.610410271878308	27.02321739421862
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	10.5
2	11.0
3	26.0
4	41.0
5	41.0
6	59.5
7	78.0
8	76.5
9	75.0
10	128.0
11	181.0
12	181.0
13	298.5
14	416.0
15	701.5
16	987.0
17	1071.5
18	1156.0
19	1156.0
20	1461.0
21	1766.0
22	2256.5
23	2747.0
24	3416.5
25	4086.0
26	4086.0
27	4850.5
28	5615.0
29	7385.0
30	9155.0
31	10539.0
32	11923.0
33	11923.0
34	13601.5
35	15280.0
36	17138.5
37	18997.0
38	21373.5
39	23750.0
40	23750.0
41	26133.5
42	28517.0
43	28884.0
44	29251.0
45	30898.0
46	32545.0
47	32545.0
48	34041.5
49	35538.0
50	37630.5
51	39723.0
52	42174.5
53	44626.0
54	44626.0
55	42139.5
56	39653.0
57	38111.5
58	36570.0
59	33560.5
60	30551.0
61	30551.0
62	28405.5
63	26260.0
64	23487.5
65	20715.0
66	17965.5
67	15216.0
68	15216.0
69	12965.5
70	10715.0
71	9339.5
72	7964.0
73	6534.0
74	5104.0
75	5104.0
76	4157.5
77	3211.0
78	2706.5
79	2202.0
80	1725.0
81	1248.0
82	1248.0
83	951.5
84	655.0
85	524.5
86	394.0
87	291.0
88	188.0
89	188.0
90	143.5
91	99.0
92	59.5
93	20.0
94	13.5
95	7.0
96	7.0
97	5.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	507249.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.22001594481515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.89296101071032	68.20297031431195
2	4.798766640314234	7.123290731191388
3	1.1646482979668396	2.593206457356019
4	0.5279498990720781	1.5673779970877273
5	0.33166814503334074	1.2308207506380906
6	0.21893016030640208	0.9749399993245261
7	0.1486296208200759	0.7721904978996502
8	0.11558371275514041	0.6862900002917964
9	0.07858179537150468	0.524910789490075
>10	0.6208492243030623	9.306375763075899
>50	0.07514427606823018	3.842362134683408
>100	0.026287217278806584	3.175264564649444
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9714183763792535E-4
2	0.0	0.0	0.0	0.0	1.9714183763792535E-4
3	0.0	0.0	0.0	0.0	1.9714183763792535E-4
4	0.0	0.0	0.0	0.0	1.9714183763792535E-4
5	0.0	0.0	0.0	0.0	1.9714183763792535E-4
6	0.0	0.0	0.0	0.0	1.9714183763792535E-4
7	0.0	0.0	0.0	0.0	1.9714183763792535E-4
8	0.0	0.0	0.0	0.0	1.9714183763792535E-4
9	1.9714183763792535E-4	0.0	0.0	0.0	1.9714183763792535E-4
10	1.9714183763792535E-4	0.0	0.0	0.0	1.9714183763792535E-4
11	1.9714183763792535E-4	0.0	0.0	0.0	1.9714183763792535E-4
12	1.9714183763792535E-4	0.0	0.0	0.0	1.9714183763792535E-4
13	1.9714183763792535E-4	0.0	0.0	1.9714183763792535E-4	1.9714183763792535E-4
14	1.9714183763792535E-4	0.0	0.0	1.9714183763792535E-4	1.9714183763792535E-4
15	1.9714183763792535E-4	0.0	0.0	1.9714183763792535E-4	1.9714183763792535E-4
16	1.9714183763792535E-4	0.0	0.0	1.9714183763792535E-4	1.9714183763792535E-4
17	1.9714183763792535E-4	0.0	0.0	1.9714183763792535E-4	1.9714183763792535E-4
18	1.9714183763792535E-4	0.0	0.0	1.9714183763792535E-4	1.9714183763792535E-4
19	1.9714183763792535E-4	0.0	0.0	3.942836752758507E-4	1.9714183763792535E-4
20	1.9714183763792535E-4	0.0	0.0	5.91425512913776E-4	1.9714183763792535E-4
21	1.9714183763792535E-4	0.0	0.0	5.91425512913776E-4	1.9714183763792535E-4
22	1.9714183763792535E-4	0.0	0.0	0.001182851025827552	1.9714183763792535E-4
23	1.9714183763792535E-4	0.0	0.0	0.0019714183763792537	1.9714183763792535E-4
24	3.942836752758507E-4	0.0	0.0	0.0037456949151205817	1.9714183763792535E-4
25	3.942836752758507E-4	0.0	0.0	0.005914255129137761	1.9714183763792535E-4
26	3.942836752758507E-4	0.0	0.0	0.009857091881896268	1.9714183763792535E-4
27	3.942836752758507E-4	0.0	0.0	0.040216934878136774	1.9714183763792535E-4
28	3.942836752758507E-4	0.0	0.0	0.1305078965163066	1.9714183763792535E-4
29	3.942836752758507E-4	0.0	0.0	0.2606215093573373	1.9714183763792535E-4
30	3.942836752758507E-4	0.0	0.0	0.44179485814659075	1.9714183763792535E-4
31	3.942836752758507E-4	0.0	0.0	1.001283393363023	1.9714183763792535E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGT	30	3.596668E-4	30.833334	35
TGCGTTA	30	3.596668E-4	30.833334	37
GTAATAC	40	5.9336875E-5	27.75	3
GGTATCA	195	0.0	27.512821	1
ATACTGA	35	8.862534E-4	26.428572	6
CGTGTTG	40	0.0019297079	23.125	24
ATGCGTT	40	0.0019297079	23.125	36
GTTATAC	40	0.0019297079	23.125	1
CGGACAT	110	7.4578566E-11	21.863636	21
AGAGGTC	150	0.0	20.966665	8
GTCGGGA	65	6.892415E-5	19.923077	2
CTTATAC	560	0.0	19.491072	37
TCGCCAT	155	1.8189894E-12	19.096775	13
GTATCAA	295	0.0	18.813559	2
TCTTACC	60	9.2271186E-4	18.5	2
GGATACC	50	0.007029595	18.5	25
GTGTGCG	60	9.2271186E-4	18.5	9
GCGGGTA	60	9.2271186E-4	18.5	23
GACGGAC	50	0.007029595	18.5	7
ATTTGCG	80	1.6137703E-5	18.5	16
>>END_MODULE