##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632148.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	821974
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.682256373072626	34.0	31.0	34.0	31.0	34.0
2	32.85188947582284	34.0	31.0	34.0	31.0	34.0
3	32.94322934788691	34.0	31.0	34.0	31.0	34.0
4	36.36211850983121	37.0	37.0	37.0	35.0	37.0
5	36.21903855839722	37.0	37.0	37.0	35.0	37.0
6	36.23701114633796	37.0	37.0	37.0	35.0	37.0
7	36.217348723925575	37.0	36.0	37.0	35.0	37.0
8	36.19997834481383	37.0	36.0	37.0	35.0	37.0
9	37.98573555854565	39.0	38.0	39.0	35.0	39.0
10	37.93509040431936	39.0	38.0	39.0	35.0	39.0
11	38.00832629742547	39.0	38.0	39.0	35.0	39.0
12	37.959064398630616	39.0	38.0	39.0	35.0	39.0
13	37.988106679773324	39.0	38.0	39.0	35.0	39.0
14	39.38023222145713	40.0	39.0	41.0	37.0	41.0
15	39.38688571657984	40.0	39.0	41.0	37.0	41.0
16	39.38716066444924	40.0	39.0	41.0	37.0	41.0
17	39.363178154053536	40.0	39.0	41.0	37.0	41.0
18	39.348532921965905	40.0	39.0	41.0	36.0	41.0
19	39.372447303686	40.0	39.0	41.0	37.0	41.0
20	39.34424933148737	40.0	39.0	41.0	36.0	41.0
21	39.319377498558346	40.0	39.0	41.0	36.0	41.0
22	39.27987016620963	40.0	39.0	41.0	36.0	41.0
23	39.23595150211564	40.0	39.0	41.0	36.0	41.0
24	39.19404141736843	40.0	39.0	41.0	36.0	41.0
25	39.134885045025754	40.0	39.0	41.0	36.0	41.0
26	39.047833629774175	40.0	39.0	41.0	36.0	41.0
27	38.97254657689903	40.0	39.0	41.0	35.0	41.0
28	38.91194368678328	40.0	38.0	41.0	35.0	41.0
29	38.8439865494529	40.0	38.0	41.0	35.0	41.0
30	38.781918892811696	40.0	38.0	41.0	35.0	41.0
31	38.70908690542523	40.0	38.0	41.0	35.0	41.0
32	38.623525804952465	40.0	38.0	41.0	35.0	41.0
33	38.54818278923664	40.0	38.0	41.0	35.0	41.0
34	38.46879098365642	40.0	38.0	41.0	34.0	41.0
35	38.44742777752094	40.0	38.0	41.0	34.0	41.0
36	38.4066211340018	40.0	38.0	41.0	34.0	41.0
37	38.343905038358876	40.0	38.0	41.0	34.0	41.0
38	38.30078566961972	40.0	38.0	41.0	34.0	41.0
39	38.2437120882169	40.0	38.0	41.0	34.0	41.0
40	38.181963176450836	40.0	38.0	41.0	34.0	41.0
41	38.13508578130209	40.0	37.0	41.0	34.0	41.0
42	38.07860589264381	40.0	37.0	41.0	34.0	41.0
43	37.19244161007526	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	1.0
15	2.0
16	3.0
17	5.0
18	6.0
19	21.0
20	53.0
21	101.0
22	173.0
23	329.0
24	562.0
25	933.0
26	1345.0
27	1947.0
28	2833.0
29	3987.0
30	5735.0
31	7840.0
32	10609.0
33	14681.0
34	21860.0
35	32297.0
36	51165.0
37	91559.0
38	220199.0
39	353726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.16818536839365	16.446870582281193	11.361064948526352	28.02387910079881
2	19.74089204768034	18.86860168326492	32.23423125305666	29.156275015998073
3	20.31098307245728	20.085428492872037	28.497738371286687	31.105850063384
4	15.712297469263017	14.347655765267515	32.03933457749272	37.90071218797675
5	17.15808529223552	33.52052498010886	32.70212926442929	16.619260463226333
6	38.09986690576588	32.06098976366649	14.60776131605136	15.231382014516273
7	32.57986262339198	28.119015929944258	19.237469798314788	20.063651648348973
8	29.913598240333634	31.223737003846836	18.47503692330901	20.387627832510518
9	28.205028382892888	13.155038967169277	17.377070320959056	41.26286232897878
10	18.144856163333635	24.968794633406898	31.3013550306944	25.584994172565068
11	39.455627550263145	19.379566750286507	19.53857421280965	21.626231486640695
12	24.17692043787273	22.70582767824773	27.393323876424315	25.72392800745522
13	33.319180412032495	17.325243864161152	21.88147070345291	27.47410502035344
14	24.570606856177932	17.83499234768983	22.705097728151983	34.88930306798025
15	27.518996951241764	24.75443262195641	19.99430638925319	27.73226403754863
16	27.723991269796855	23.333219785540663	21.640343855158438	27.302445089504047
17	26.518357028324495	23.51205755899822	23.034061904634452	26.935523508042834
18	26.87372106660308	22.724563064038524	23.66936180463129	26.732354064727108
19	27.46108757697932	22.844761513137886	23.162411463136305	26.53173944674649
20	27.538218970429725	22.78405399684175	22.985885198315277	26.691841834413253
21	27.983488528834243	22.65022981262181	22.58757576273702	26.778705895806926
22	28.288364352157124	22.508741152396546	22.35788479927589	26.84500969617044
23	27.74175338879332	22.509349444143	22.572490127424956	27.176407039638722
24	27.5225250433712	22.609595923958665	22.4149425650933	27.452936467576833
25	27.491988797699197	22.11541970913922	22.870188108139676	27.52240338502191
26	27.213391177823144	22.456914695598645	22.907050587974805	27.42264353860341
27	27.139666218152886	22.43781433475998	22.914106772233673	27.50841267485346
28	27.165457788202545	22.548645090963948	22.836610403735396	27.449286717098108
29	26.956935377518022	22.934423716565245	23.05815025779404	27.05049064812269
30	26.844279746074694	23.05194568198021	22.88928846897834	27.214486102966763
31	27.289549304479216	22.829067586079365	22.674804799178563	27.20657831026286
32	26.52565652928195	23.042821305783395	23.030898787552893	27.400623377381766
33	26.500716567677323	22.676994649465797	23.572765075294352	27.249523707562528
34	27.018859477307068	22.15009233868711	23.471910303732237	27.359137880273586
35	26.54597347361352	22.4588612291873	23.925087654840663	27.07007764235852
36	26.61799521639371	22.337567854944318	23.801482771961158	27.242954156700822
37	26.31676914354955	21.86796662668162	24.1440726835642	27.671191546204625
38	25.779890848129018	21.962860139128487	24.763435339803934	27.49381367293856
39	25.53280274071929	21.55396642716193	25.52781474839837	27.38541608372041
40	25.492168852056146	21.342767532793008	25.91724312447839	27.247820490672453
41	24.961616790798736	21.05382895322723	26.700601235562193	27.28395302041184
42	24.442135639326793	21.169404385053543	27.05182888996489	27.336631085654776
43	23.88883346675199	20.547851878526572	27.587003968495356	27.976310686226086
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	7.0
2	5.0
3	39.5
4	74.0
5	74.0
6	86.0
7	98.0
8	103.5
9	109.0
10	138.5
11	168.0
12	168.0
13	274.0
14	380.0
15	682.5
16	985.0
17	1062.0
18	1139.0
19	1139.0
20	1423.5
21	1708.0
22	1897.0
23	2086.0
24	2794.5
25	3503.0
26	3503.0
27	4448.0
28	5393.0
29	6727.5
30	8062.0
31	9794.0
32	11526.0
33	11526.0
34	13896.5
35	16267.0
36	19034.0
37	21801.0
38	24939.0
39	28077.0
40	28077.0
41	31741.0
42	35405.0
43	38933.0
44	42461.0
45	45819.0
46	49177.0
47	49177.0
48	52393.0
49	55609.0
50	58466.5
51	61324.0
52	63935.5
53	66547.0
54	66547.0
55	66727.0
56	66907.0
57	66542.5
58	66178.0
59	65058.0
60	63938.0
61	63938.0
62	60133.0
63	56328.0
64	50821.5
65	45315.0
66	40128.0
67	34941.0
68	34941.0
69	30478.5
70	26016.0
71	22221.0
72	18426.0
73	15348.5
74	12271.0
75	12271.0
76	10051.5
77	7832.0
78	6461.5
79	5091.0
80	4204.5
81	3318.0
82	3318.0
83	2580.0
84	1842.0
85	1389.5
86	937.0
87	693.5
88	450.0
89	450.0
90	327.0
91	204.0
92	124.5
93	45.0
94	28.0
95	11.0
96	11.0
97	8.0
98	5.0
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	821974.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.74972849256017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.08202828850528	65.84107699639775
2	7.164436357455327	10.71079345044025
3	1.9055753949922611	4.2732373019332375
4	0.8595305352430965	2.569986965619455
5	0.49717869595111924	1.858198626731564
6	0.3126944464452908	1.402429498375013
7	0.2125701437848316	1.1122692373478447
8	0.15580475312090653	0.9317090394910502
9	0.12328373837343035	0.8293883373865565
>10	0.6572721954991664	8.745755251338519
>50	0.025882184871970346	1.2593356445908717
>100	0.0037432657573096516	0.4658196503479411
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2165834929085348E-4
2	0.0	0.0	0.0	0.0	1.2165834929085348E-4
3	0.0	0.0	0.0	0.0	1.2165834929085348E-4
4	0.0	0.0	0.0	1.2165834929085348E-4	1.2165834929085348E-4
5	0.0	0.0	0.0	1.2165834929085348E-4	1.2165834929085348E-4
6	0.0	0.0	0.0	1.2165834929085348E-4	1.2165834929085348E-4
7	0.0	0.0	0.0	1.2165834929085348E-4	1.2165834929085348E-4
8	0.0	0.0	0.0	1.2165834929085348E-4	1.2165834929085348E-4
9	0.0	2.4331669858170695E-4	0.0	1.2165834929085348E-4	1.2165834929085348E-4
10	0.0	2.4331669858170695E-4	0.0	1.2165834929085348E-4	1.2165834929085348E-4
11	0.0	2.4331669858170695E-4	0.0	1.2165834929085348E-4	1.2165834929085348E-4
12	0.0	2.4331669858170695E-4	0.0	1.2165834929085348E-4	1.2165834929085348E-4
13	0.0	2.4331669858170695E-4	0.0	2.4331669858170695E-4	1.2165834929085348E-4
14	0.0	2.4331669858170695E-4	0.0	2.4331669858170695E-4	1.2165834929085348E-4
15	0.0	2.4331669858170695E-4	0.0	3.6497504787256043E-4	1.2165834929085348E-4
16	0.0	2.4331669858170695E-4	0.0	4.866333971634139E-4	1.2165834929085348E-4
17	0.0	2.4331669858170695E-4	0.0	4.866333971634139E-4	1.2165834929085348E-4
18	0.0	2.4331669858170695E-4	0.0	4.866333971634139E-4	1.2165834929085348E-4
19	0.0	2.4331669858170695E-4	0.0	4.866333971634139E-4	1.2165834929085348E-4
20	0.0	2.4331669858170695E-4	0.0	4.866333971634139E-4	1.2165834929085348E-4
21	0.0	2.4331669858170695E-4	0.0	4.866333971634139E-4	1.2165834929085348E-4
22	0.0	2.4331669858170695E-4	0.0	8.516084450359743E-4	1.2165834929085348E-4
23	0.0	2.4331669858170695E-4	0.0	0.0015815585407810953	1.2165834929085348E-4
24	0.0	2.4331669858170695E-4	0.0	0.0024331669858170695	1.2165834929085348E-4
25	0.0	2.4331669858170695E-4	0.0	0.0030414587322713372	1.2165834929085348E-4
26	0.0	2.4331669858170695E-4	0.0	0.004136383875889018	1.2165834929085348E-4
27	0.0	2.4331669858170695E-4	0.0	0.01849206909220973	1.2165834929085348E-4
28	0.0	2.4331669858170695E-4	0.0	0.07652310170394684	1.2165834929085348E-4
29	0.0	2.4331669858170695E-4	0.0	0.15961575426959976	1.2165834929085348E-4
30	0.0	2.4331669858170695E-4	0.0	0.26132213427675327	1.2165834929085348E-4
31	0.0	2.4331669858170695E-4	0.0	0.5873665103762407	1.2165834929085348E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	285	0.0	28.561405	1
TCTATTC	75	9.260506E-6	19.733334	3
TTACACA	85	1.2444252E-6	19.588236	4
CTTATAC	515	0.0	19.398058	37
TAATAGA	60	9.2337653E-4	18.5	4
ATTATCC	85	2.7215878E-5	17.411764	3
GTATCAA	465	0.0	17.107527	2
TAGGACT	175	1.3096724E-10	15.857143	4
TCGCACG	95	7.055765E-5	15.578948	22
TATACAC	215	0.0	15.488372	37
CTTAAGA	85	5.361843E-4	15.235294	2
TAAGCTC	85	5.361843E-4	15.235294	5
CTAGAAC	75	0.004103083	14.8	3
CTATACT	100	1.0927618E-4	14.8	4
TAGATAA	100	1.0927618E-4	14.8	4
TAAATAG	75	0.004103083	14.8	5
TAAGACT	75	0.004103083	14.8	4
TCTTATA	865	0.0	14.543352	37
TAGAAGT	115	2.2082202E-5	14.47826	4
TTGTACC	105	1.654725E-4	14.095239	4
>>END_MODULE