FastQCFastQC Report
Fri 10 Feb 2017
ERR1632147.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632147.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3663350
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT43150.11778836311026793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA12650.022.229254
TCTAGAC12650.021.4980243
GGTATCA14400.021.0694451
GTTCTAG14050.020.4092521
GTCTAGA10050.019.328361
TAGAACT11800.018.8135594
CTTATAC28200.018.537
CTAGAAC12100.018.1942143
TAGACAT15450.017.9611665
ACATGTA9800.017.74498
GCAATTA10650.017.7183117
CGCTATA852.7257498E-517.4117642
CTCTAGT14800.017.12527
TTCTAGA16700.017.059882
ACTGATC9600.016.9583348
TCTTATA51050.016.3800237
TCTAGAA12450.016.345382
CCTCTAG16650.016.33333426
CAATTAC11450.016.31877718
GTATCAA18950.015.9129291
Produced by FastQC (version 0.11.5)