FastQCFastQC Report
Fri 10 Feb 2017
ERR1632146.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632146.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7135611
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88920.12461441634080109No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT83140.11651419899431177No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA77960.10925483465956876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT75810.10624177803414453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37100.023.9851741
TCTAGAC24750.021.602023
GTCTAGA23800.021.3760491
TAGAACT26400.021.3731064
GTTCTAG26150.020.7992341
CTAGAAC25950.020.46053
ACTGATC20000.019.4258
CTAGACA28900.019.1401394
GCAATTA25950.018.60693617
CTCTAGT27400.018.16240927
CTTATAC69950.017.56111537
ACGGAGT21500.016.69302226
TCTAGAA32600.016.5138052
GCCTCTA22050.016.10884525
CAATTAC30300.015.93564418
TAGACAT34700.015.8876085
GTATCAA55350.015.8762432
ACATGTA23100.015.6168838
CCTCTAG38150.015.56618626
TTCTAGA35800.015.554472
Produced by FastQC (version 0.11.5)