##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632146.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7135611
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.854115225731896	34.0	31.0	34.0	31.0	34.0
2	33.01473230533447	34.0	33.0	34.0	31.0	34.0
3	33.10264082501134	34.0	33.0	34.0	31.0	34.0
4	36.464621179601856	37.0	37.0	37.0	35.0	37.0
5	36.3481442584244	37.0	37.0	37.0	35.0	37.0
6	36.37239572056268	37.0	37.0	37.0	35.0	37.0
7	36.350486174204285	37.0	37.0	37.0	35.0	37.0
8	36.33884722695786	37.0	37.0	37.0	35.0	37.0
9	38.13975425510163	39.0	39.0	39.0	37.0	39.0
10	38.11913555825843	39.0	38.0	39.0	37.0	39.0
11	38.177218881466494	39.0	39.0	39.0	37.0	39.0
12	38.1450111279889	39.0	39.0	39.0	37.0	39.0
13	38.161076185346985	39.0	39.0	39.0	37.0	39.0
14	39.5940584485337	41.0	39.0	41.0	37.0	41.0
15	39.60526477690558	41.0	39.0	41.0	37.0	41.0
16	39.606621633382204	41.0	39.0	41.0	37.0	41.0
17	39.596992044549516	41.0	39.0	41.0	37.0	41.0
18	39.593224742772556	41.0	39.0	41.0	37.0	41.0
19	39.61161363757077	41.0	39.0	41.0	37.0	41.0
20	39.57752363462638	41.0	39.0	41.0	37.0	41.0
21	39.55096304997568	41.0	39.0	41.0	37.0	41.0
22	39.51654105023382	41.0	39.0	41.0	37.0	41.0
23	39.46853871378359	41.0	39.0	41.0	37.0	41.0
24	39.42572219814112	41.0	39.0	41.0	37.0	41.0
25	39.37555971030371	41.0	39.0	41.0	37.0	41.0
26	39.30177065425792	40.0	39.0	41.0	36.0	41.0
27	39.22499923272163	40.0	39.0	41.0	36.0	41.0
28	39.1615397756408	40.0	39.0	41.0	36.0	41.0
29	39.10319438657741	40.0	39.0	41.0	36.0	41.0
30	39.0378324995575	40.0	39.0	41.0	35.0	41.0
31	38.9690210130569	40.0	39.0	41.0	35.0	41.0
32	38.87826003967985	40.0	38.0	41.0	35.0	41.0
33	38.796780401846455	40.0	38.0	41.0	35.0	41.0
34	38.72176846523725	40.0	38.0	41.0	35.0	41.0
35	38.68751631780376	40.0	38.0	41.0	35.0	41.0
36	38.643210651477496	40.0	38.0	41.0	35.0	41.0
37	38.57982168590748	40.0	38.0	41.0	35.0	41.0
38	38.522738557356895	40.0	38.0	41.0	35.0	41.0
39	38.45946310694347	40.0	38.0	41.0	35.0	41.0
40	38.37574483810847	40.0	38.0	41.0	34.0	41.0
41	38.31593216053958	40.0	38.0	41.0	34.0	41.0
42	38.237581897331566	40.0	38.0	41.0	34.0	41.0
43	37.37350340426349	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	10.0
11	9.0
12	4.0
13	1.0
14	3.0
15	7.0
16	15.0
17	30.0
18	73.0
19	154.0
20	341.0
21	667.0
22	1241.0
23	2300.0
24	3781.0
25	6136.0
26	9515.0
27	13917.0
28	20430.0
29	28991.0
30	40737.0
31	56016.0
32	76270.0
33	105702.0
34	157027.0
35	236654.0
36	388464.0
37	730650.0
38	1740924.0
39	3515538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.95430188108629	17.2279991159832	11.41935848240606	27.39834052052445
2	19.45187314723294	19.75487453001572	33.055557540902946	27.737694781848393
3	20.341089221371515	20.349091339199965	28.701172191141023	30.608647248287497
4	15.347935867019658	14.432036723975003	32.981744660688484	37.238282748316855
5	16.793320151560952	34.0918528210128	32.29678579731995	16.818041230106292
6	36.542532937964246	33.31198968105184	14.699610166529537	15.445867214454376
7	31.700747139943587	28.64578800610067	19.59181911682125	20.06164573713449
8	28.322592136819118	31.62415944479036	19.378789006295328	20.674459412095196
9	27.348519979578484	13.571535780187569	18.52298282515681	40.55696141507714
10	18.173804597812296	25.032390358723312	31.19067168880142	25.603133354662972
11	38.15338308100035	20.098559184350158	20.484160361320143	21.26389737332935
12	23.691173748120516	23.7803882526668	27.154072720612156	25.37436527860053
13	31.778091602807386	17.893422161045493	23.57451940695758	26.75396682918954
14	24.121312106279337	18.805173095898866	23.201923423235936	33.87159137458586
15	27.016270926203795	25.482442919043653	20.903269530808224	26.598016623944325
16	26.7568537578632	24.208802862151536	22.38458626738481	26.649757112600447
17	25.796669689533243	24.328960757530083	23.532224500466743	26.342145052469927
18	26.167289668677284	23.14922996783317	24.293168447663415	26.390311915826132
19	26.43618044761689	23.325711000781855	24.028481933782544	26.20962661781871
20	26.44699942303469	23.503649512284234	23.99114806006101	26.05820300462007
21	27.081352949312958	23.42142810195231	23.853094009749128	25.644124938985602
22	27.3950752079955	23.047458724978142	23.214788474315654	26.3426775927107
23	26.407311160880266	23.446121152063924	23.706925727873898	26.439641959181913
24	26.304474837543694	23.436129015441004	23.355140856192975	26.904255290822327
25	26.721636030887897	23.011722471978924	23.770199917007808	26.496441580125374
26	26.67885062680687	23.224640468769948	23.599058300683712	26.49745060373947
27	26.171802246506992	23.228592477925154	23.777585409294314	26.82201986627354
28	26.039269797638916	22.927721256105468	24.408785736778533	26.624223209477087
29	25.76187238906381	23.49875855059924	24.13828332289975	26.601085737437202
30	25.52207512433063	23.949231537425455	24.3148904838002	26.213802854443717
31	26.63413126079883	23.306175182475613	23.53570002624863	26.52399353047693
32	25.911698381540138	23.445966995678436	23.804562776754505	26.83777184602692
33	25.79893999266496	22.959715713202417	24.59126485454434	26.650079439588286
34	26.701749857160095	22.40273467822167	24.30583729970706	26.589678164911174
35	25.933588588279267	22.94347323585885	24.966425439951813	26.15651273591007
36	25.831635160605025	22.40527125147377	25.115004167127385	26.64808942079382
37	25.792451410257648	22.163554039030434	25.15631807843785	26.887676472274062
38	25.20396641577014	21.96949917813625	26.134230131098796	26.69230427499481
39	25.247788871898987	21.842166003724138	26.31586839585286	26.594176728524015
40	24.73039799955463	22.296675085006736	26.74794071593869	26.224986199499945
41	24.664222867530196	21.809568935302106	27.16228505169354	26.363923145474153
42	24.489283398436378	22.1599103426462	27.20948493408623	26.14132132483119
43	23.64452042018546	21.621666315610536	27.415984419554263	27.317828844649743
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	306.0
1	384.5
2	463.0
3	1068.0
4	1673.0
5	1673.0
6	2113.5
7	2554.0
8	2461.5
9	2369.0
10	3394.5
11	4420.0
12	4420.0
13	6943.5
14	9467.0
15	13841.0
16	18215.0
17	17875.0
18	17535.0
19	17535.0
20	21020.0
21	24505.0
22	24662.0
23	24819.0
24	29313.5
25	33808.0
26	33808.0
27	42148.0
28	50488.0
29	63472.0
30	76456.0
31	92925.0
32	109394.0
33	109394.0
34	137175.0
35	164956.0
36	197853.5
37	230751.0
38	255828.5
39	280906.0
40	280906.0
41	308288.0
42	335670.0
43	370464.5
44	405259.0
45	440887.5
46	476516.0
47	476516.0
48	490250.5
49	503985.0
50	529604.0
51	555223.0
52	564960.0
53	574697.0
54	574697.0
55	563483.0
56	552269.0
57	544504.5
58	536740.0
59	529213.5
60	521687.0
61	521687.0
62	494458.5
63	467230.0
64	418335.0
65	369440.0
66	326428.0
67	283416.0
68	283416.0
69	235851.5
70	188287.0
71	158791.5
72	129296.0
73	101645.0
74	73994.0
75	73994.0
76	58176.0
77	42358.0
78	34199.5
79	26041.0
80	21533.5
81	17026.0
82	17026.0
83	13320.0
84	9614.0
85	8191.0
86	6768.0
87	5579.5
88	4391.0
89	4391.0
90	3256.0
91	2121.0
92	1232.0
93	343.0
94	221.5
95	100.0
96	100.0
97	72.0
98	44.0
99	27.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7135611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.00894538912433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.82185250450327	33.53231842720415
2	10.291116190103308	8.64637876046366
3	3.340333748342462	4.209716940467023
4	1.6660881455658427	2.799624236821716
5	0.9729816113623153	2.0436965688170843
6	0.6497394606031985	1.637692171058332
7	0.4621613583756475	1.3590437885476263
8	0.3630742087346231	1.2201891685545845
9	0.2797748380253334	1.0577741302671604
>10	1.7960495992846568	14.416616032932442
>50	0.18723563986767539	5.495839472476846
>100	0.14273311267997452	11.944766157211054
>500	0.018428545609177484	5.38017160862673
>1k	0.00826441565929658	5.7014274749544835
>5k	1.6662128338630893E-4	0.5547450615970476
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8892	0.12461441634080109	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	8314	0.11651419899431177	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	7796	0.10925483465956876	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7581	0.10624177803414453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4014216862438268E-5	1.4014216862438268E-5
2	0.0	0.0	0.0	1.4014216862438268E-5	1.4014216862438268E-5
3	0.0	0.0	0.0	1.4014216862438268E-5	1.4014216862438268E-5
4	0.0	0.0	0.0	1.4014216862438268E-5	1.4014216862438268E-5
5	0.0	1.4014216862438268E-5	0.0	1.4014216862438268E-5	1.4014216862438268E-5
6	0.0	1.4014216862438268E-5	0.0	1.4014216862438268E-5	4.204265058731481E-5
7	0.0	1.4014216862438268E-5	0.0	2.8028433724876537E-5	4.204265058731481E-5
8	0.0	1.4014216862438268E-5	0.0	2.8028433724876537E-5	5.6056867449753074E-5
9	0.0	2.8028433724876537E-5	0.0	1.1211373489950615E-4	5.6056867449753074E-5
10	0.0	2.8028433724876537E-5	0.0	1.821848192116975E-4	5.6056867449753074E-5
11	0.0	2.8028433724876537E-5	0.0	2.3824168666145058E-4	7.007108431219134E-5
12	0.0	2.8028433724876537E-5	0.0	3.503554215609567E-4	7.007108431219134E-5
13	0.0	4.204265058731481E-5	0.0	4.064122890107098E-4	7.007108431219134E-5
14	0.0	4.204265058731481E-5	0.0	4.484549395980246E-4	7.007108431219134E-5
15	0.0	5.6056867449753074E-5	0.0	5.465544576350925E-4	7.007108431219134E-5
16	0.0	5.6056867449753074E-5	0.0	7.2873927684679E-4	7.007108431219134E-5
17	0.0	5.6056867449753074E-5	0.0	9.669809635082405E-4	7.007108431219134E-5
18	0.0	8.408530117462962E-5	0.0	0.0010790946984077466	8.408530117462962E-5
19	0.0	8.408530117462962E-5	0.0	0.0012332510838945676	8.408530117462962E-5
20	0.0	8.408530117462962E-5	0.0	0.0015275496380057713	8.408530117462962E-5
21	0.0	8.408530117462962E-5	0.0	0.001821848192116975	8.408530117462962E-5
22	0.0	8.408530117462962E-5	0.0	0.0023684026497520676	8.408530117462962E-5
23	0.0	9.809951803706788E-5	0.0	0.0035455968661968823	8.408530117462962E-5
24	0.0	9.809951803706788E-5	0.0	0.005073146504202653	8.408530117462962E-5
25	0.0	9.809951803706788E-5	0.0	0.006909008913182067	9.809951803706788E-5
26	0.0	9.809951803706788E-5	0.0	0.010314463610754566	9.809951803706788E-5
27	0.0	9.809951803706788E-5	0.0	0.03333982191574064	9.809951803706788E-5
28	0.0	1.2612795176194442E-4	0.0	0.11397762574221044	9.809951803706788E-5
29	0.0	1.401421686243827E-4	0.0	0.22932864473693984	9.809951803706788E-5
30	0.0	1.401421686243827E-4	0.0	0.3960838111830928	9.809951803706788E-5
31	1.4014216862438268E-5	1.401421686243827E-4	0.0	0.8438660683717204	9.809951803706788E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3710	0.0	23.985174	1
TCTAGAC	2475	0.0	21.60202	3
GTCTAGA	2380	0.0	21.376049	1
TAGAACT	2640	0.0	21.373106	4
GTTCTAG	2615	0.0	20.799234	1
CTAGAAC	2595	0.0	20.4605	3
ACTGATC	2000	0.0	19.425	8
CTAGACA	2890	0.0	19.140139	4
GCAATTA	2595	0.0	18.606936	17
CTCTAGT	2740	0.0	18.162409	27
CTTATAC	6995	0.0	17.561115	37
ACGGAGT	2150	0.0	16.693022	26
TCTAGAA	3260	0.0	16.513805	2
GCCTCTA	2205	0.0	16.108845	25
CAATTAC	3030	0.0	15.935644	18
TAGACAT	3470	0.0	15.887608	5
GTATCAA	5535	0.0	15.876243	2
ACATGTA	2310	0.0	15.616883	8
CCTCTAG	3815	0.0	15.566186	26
TTCTAGA	3580	0.0	15.55447	2
>>END_MODULE