FastQCFastQC Report
Fri 10 Feb 2017
ERR1632145.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632145.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619744
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42230.16119895684463825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31450.12004989800530129No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31440.12001172633661916No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT27990.10684250064128403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16600.025.1867471
ACCGTTA400.001931843523.1258
TCTAGAC7250.021.4344833
GTCTAGA7850.021.2101921
CTAGACA7600.021.1776314
TAGAACT8600.020.866284
ACATGTA6000.020.0416668
ACTGATC6500.019.9230798
CTAGAAC7900.019.9050643
CTTATAC34100.019.31378437
GCCTCTA6650.018.08270625
ACGGAGT7050.017.5815626
GACATGT7000.017.4428587
GTTCTAG9100.017.280221
TATACAC10000.017.0237
TCTAGTT8650.016.89595428
ATACACA7050.016.53191637
CTCTAGT9100.016.46703327
GTATCAA24750.016.1454542
TCTTATA60000.016.03333537
Produced by FastQC (version 0.11.5)