##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632145.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2619744
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.777874097621755	34.0	31.0	34.0	31.0	34.0
2	32.94345019971417	34.0	31.0	34.0	31.0	34.0
3	33.0324317948624	34.0	33.0	34.0	31.0	34.0
4	36.42225843441191	37.0	37.0	37.0	35.0	37.0
5	36.290674966714306	37.0	37.0	37.0	35.0	37.0
6	36.31704433715661	37.0	37.0	37.0	35.0	37.0
7	36.29524335202218	37.0	37.0	37.0	35.0	37.0
8	36.28080148289298	37.0	37.0	37.0	35.0	37.0
9	38.0784450694419	39.0	38.0	39.0	37.0	39.0
10	38.04661944067817	39.0	38.0	39.0	35.0	39.0
11	38.11588422380202	39.0	38.0	39.0	37.0	39.0
12	38.08257944287686	39.0	38.0	39.0	37.0	39.0
13	38.10027010272759	39.0	38.0	39.0	37.0	39.0
14	39.49823303345671	40.0	39.0	41.0	37.0	41.0
15	39.50074472925599	40.0	39.0	41.0	37.0	41.0
16	39.51283102471081	40.0	39.0	41.0	37.0	41.0
17	39.50418666862106	40.0	39.0	41.0	37.0	41.0
18	39.50657621508056	41.0	39.0	41.0	37.0	41.0
19	39.525359348088976	41.0	39.0	41.0	37.0	41.0
20	39.49996946266506	41.0	39.0	41.0	37.0	41.0
21	39.47118802447873	40.0	39.0	41.0	37.0	41.0
22	39.43735952825925	40.0	39.0	41.0	37.0	41.0
23	39.39453855033164	40.0	39.0	41.0	37.0	41.0
24	39.3525436073143	40.0	39.0	41.0	36.0	41.0
25	39.30091604370504	40.0	39.0	41.0	36.0	41.0
26	39.23335638902122	40.0	39.0	41.0	36.0	41.0
27	39.157639830456716	40.0	39.0	41.0	36.0	41.0
28	39.0918818785347	40.0	39.0	41.0	36.0	41.0
29	39.04153764642652	40.0	39.0	41.0	36.0	41.0
30	38.975534632391565	40.0	39.0	41.0	35.0	41.0
31	38.911767714708	40.0	38.0	41.0	35.0	41.0
32	38.8292531636679	40.0	38.0	41.0	35.0	41.0
33	38.75897644960729	40.0	38.0	41.0	35.0	41.0
34	38.693575021070764	40.0	38.0	41.0	35.0	41.0
35	38.66719267226111	40.0	38.0	41.0	35.0	41.0
36	38.63691872182931	40.0	38.0	41.0	35.0	41.0
37	38.58819105988982	40.0	38.0	41.0	35.0	41.0
38	38.55045531166404	40.0	38.0	41.0	35.0	41.0
39	38.51104802606667	40.0	38.0	41.0	35.0	41.0
40	38.44260126180268	40.0	38.0	41.0	35.0	41.0
41	38.39859772557929	40.0	38.0	41.0	34.0	41.0
42	38.33665846739223	40.0	38.0	41.0	34.0	41.0
43	37.496759988762264	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	2.0
13	1.0
14	4.0
15	1.0
16	6.0
17	9.0
18	24.0
19	63.0
20	136.0
21	207.0
22	446.0
23	852.0
24	1341.0
25	2142.0
26	3345.0
27	5172.0
28	7463.0
29	10958.0
30	15260.0
31	21557.0
32	29307.0
33	40552.0
34	60653.0
35	90349.0
36	147655.0
37	274633.0
38	662539.0
39	1245062.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15799559040883	17.475295296028925	11.641290141326786	27.725418972235456
2	19.326430368768857	19.74589883591679	33.4167002577351	27.510970537579244
3	20.42890450364616	20.28808921787778	28.671274750509973	30.61173152796609
4	15.366081571329108	14.538710652643921	32.32018090317222	37.77502687285475
5	16.84767671955733	34.00603265051853	32.2121169091331	16.93417372079104
6	37.006554838946094	32.7179678625087	14.799270463068147	15.476206835477054
7	31.77470775770457	28.45877307095655	19.615618930704677	20.150900240634197
8	28.383803913664845	31.677179144221725	19.33093462567335	20.60808231644008
9	27.595826157059623	13.497807419350899	18.71404228810143	40.19232413548805
10	17.99725469358838	25.433057581198774	31.19010865183774	25.37957907337511
11	38.37302423442902	20.079939108554118	20.171360255047823	21.37567640196905
12	24.085177788364053	23.770643238423297	27.146774646683035	24.997404326529615
13	31.859067145492077	17.969007658763605	23.47924071970391	26.692684476040405
14	24.422997056200913	18.910435523470994	23.157224522701455	33.50934289762664
15	26.672186289958105	26.066783624659507	20.775999486972772	26.485030598409615
16	27.019624818302855	23.985587904772373	22.20942198932415	26.78536528760062
17	25.597501129881394	24.559117226721387	23.569058656112965	26.274322987284254
18	26.30543289726019	22.92231607363162	24.45540480291204	26.31684622619615
19	26.42380324184348	23.52149675693503	23.989366899971905	26.06533310124959
20	26.51465181330695	23.557607155508325	23.912374644240046	26.015366386944677
21	27.51001624586219	23.370375120622473	24.047807724724247	25.071800908791086
22	27.43932231546288	23.045801421818314	23.105463739968485	26.409412522750316
23	26.139118936812146	23.382895427950213	23.75403856254657	26.72394707269107
24	26.120452990826585	23.4059892875029	23.50966353964357	26.963894182026948
25	26.612218598458476	23.246775257429732	23.578219856596675	26.562786287515117
26	26.56820666446798	23.255249367877166	23.676359216778433	26.50018475087642
27	26.477358093004504	23.050801910415675	23.93798783392576	26.53385216265406
28	25.818438748213566	23.11435773877142	24.28290703213749	26.784296480877522
29	25.529860932976657	23.566539325979942	23.9863895098147	26.9172102312287
30	25.11333168431725	24.01505643299498	24.614809691328617	26.256802191359156
31	26.410137784455273	23.510197943005117	23.530238069063238	26.549426203476372
32	25.56020740957895	23.27677818901389	24.083154689923898	27.079859711483262
33	25.495201057813283	22.78955500995517	24.645766914629828	27.069477017601717
34	26.315281187780183	22.212933782842903	24.711956588124643	26.759828441252274
35	25.665637558479	22.461202315951482	25.45092192214201	26.42223820342751
36	25.2368933758413	21.916645290532205	25.97738557660596	26.869075757020532
37	25.45733476248061	21.45148533597176	26.020634077222816	27.07054582432482
38	24.480598104242247	20.94613061428903	27.49192287490686	27.08134840656186
39	24.50464625551199	20.512958518084208	27.961014511341563	27.021380715062236
40	23.703461101542747	21.41171045720498	28.180577949601183	26.704250491651095
41	23.518366680103096	20.692899764251774	29.378252226171718	26.410481329473413
42	23.062978672725272	21.094045830432286	29.40359821417665	26.439377282665788
43	21.741322816275176	20.708206603393307	29.72927889137259	27.821191688958923
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	129.5
2	155.0
3	413.0
4	671.0
5	671.0
6	808.0
7	945.0
8	947.0
9	949.0
10	1426.0
11	1903.0
12	1903.0
13	2998.5
14	4094.0
15	6112.5
16	8131.0
17	7795.5
18	7460.0
19	7460.0
20	8350.0
21	9240.0
22	8815.0
23	8390.0
24	9959.5
25	11529.0
26	11529.0
27	14205.0
28	16881.0
29	20939.0
30	24997.0
31	29812.5
32	34628.0
33	34628.0
34	44631.5
35	54635.0
36	66821.5
37	79008.0
38	89015.5
39	99023.0
40	99023.0
41	109970.0
42	120917.0
43	134618.5
44	148320.0
45	163690.0
46	179060.0
47	179060.0
48	188870.5
49	198681.0
50	208344.5
51	218008.0
52	223135.5
53	228263.0
54	228263.0
55	222718.5
56	217174.0
57	208988.5
58	200803.0
59	197827.5
60	194852.0
61	194852.0
62	183307.0
63	171762.0
64	149351.5
65	126941.0
66	111437.5
67	95934.0
68	95934.0
69	78352.0
70	60770.0
71	51320.0
72	41870.0
73	31370.5
74	20871.0
75	20871.0
76	16304.5
77	11738.0
78	9548.5
79	7359.0
80	6541.0
81	5723.0
82	5723.0
83	4426.0
84	3129.0
85	2719.5
86	2310.0
87	1949.5
88	1589.0
89	1589.0
90	1181.5
91	774.0
92	439.5
93	105.0
94	67.0
95	29.0
96	29.0
97	22.5
98	16.0
99	9.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2619744.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.99403153799518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.94664633246543	35.66210728388951
2	8.138651363827512	6.9982686682629485
3	2.7736921985392873	3.5775662958206786
4	1.4121611117049686	2.42857997493495
5	0.8642879823425164	1.8579612385372184
6	0.5969306532114507	1.5398673198101502
7	0.4518495668270401	1.3598784168613853
8	0.33703492327154105	1.1592392096433937
9	0.26235373913581683	1.015168044106464
>10	1.7838015534490457	15.041490374384326
>50	0.22154222670283122	6.661830296117752
>100	0.19319331775875606	16.55592460687442
>500	0.015190100799011567	4.364470343779287
>1k	0.0026649299647388547	1.7776479269774836
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4223	0.16119895684463825	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3145	0.12004989800530129	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3144	0.12001172633661916	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	2799	0.10684250064128403	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.8171668682130776E-5	0.0	0.0	0.0
6	0.0	3.8171668682130776E-5	0.0	0.0	0.0
7	0.0	3.8171668682130776E-5	0.0	0.0	0.0
8	0.0	3.8171668682130776E-5	0.0	0.0	0.0
9	0.0	3.8171668682130776E-5	0.0	1.1451500604639232E-4	0.0
10	0.0	3.8171668682130776E-5	0.0	1.1451500604639232E-4	0.0
11	0.0	3.8171668682130776E-5	0.0	1.526866747285231E-4	0.0
12	0.0	3.8171668682130776E-5	0.0	4.580600241855693E-4	0.0
13	0.0	3.8171668682130776E-5	0.0	4.580600241855693E-4	0.0
14	0.0	3.8171668682130776E-5	0.0	4.962316928677E-4	0.0
15	0.0	3.8171668682130776E-5	0.0	6.870900362783539E-4	0.0
16	0.0	3.8171668682130776E-5	0.0	0.0010306350544175308	0.0
17	0.0	3.8171668682130776E-5	0.0	0.001336008403874577	0.0
18	0.0	3.8171668682130776E-5	0.0	0.0018704117654244079	0.0
19	0.0	3.8171668682130776E-5	0.0	0.0022139567835635847	0.0
20	0.0	3.8171668682130776E-5	0.0	0.0027865318137955462	0.0
21	0.0	3.8171668682130776E-5	0.0	0.0033972785127096387	0.0
22	0.0	3.8171668682130776E-5	0.0	0.004389741898445039	0.0
23	0.0	3.8171668682130776E-5	0.0	0.006069295320458793	0.0
24	0.0	3.8171668682130776E-5	0.0	0.008092393760611724	0.0
25	0.0	3.8171668682130776E-5	0.0	0.010115492200764655	0.0
26	0.0	3.8171668682130776E-5	0.0	0.01377997239424921	0.0
27	0.0	3.8171668682130776E-5	0.0	0.04023293879096584	0.0
28	0.0	3.8171668682130776E-5	0.0	0.1565420132654183	0.0
29	0.0	3.8171668682130776E-5	0.0	0.32606239388276104	0.0
30	0.0	3.8171668682130776E-5	0.0	0.5536418825656247	0.0
31	0.0	3.8171668682130776E-5	0.0	1.2769568324233207	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1660	0.0	25.186747	1
ACCGTTA	40	0.0019318435	23.125	8
TCTAGAC	725	0.0	21.434483	3
GTCTAGA	785	0.0	21.210192	1
CTAGACA	760	0.0	21.177631	4
TAGAACT	860	0.0	20.86628	4
ACATGTA	600	0.0	20.041666	8
ACTGATC	650	0.0	19.923079	8
CTAGAAC	790	0.0	19.905064	3
CTTATAC	3410	0.0	19.313784	37
GCCTCTA	665	0.0	18.082706	25
ACGGAGT	705	0.0	17.58156	26
GACATGT	700	0.0	17.442858	7
GTTCTAG	910	0.0	17.28022	1
TATACAC	1000	0.0	17.02	37
TCTAGTT	865	0.0	16.895954	28
ATACACA	705	0.0	16.531916	37
CTCTAGT	910	0.0	16.467033	27
GTATCAA	2475	0.0	16.145454	2
TCTTATA	6000	0.0	16.033335	37
>>END_MODULE