FastQCFastQC Report
Fri 10 Feb 2017
ERR1632144.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632144.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186188
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC9570.5139966055814553No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT8460.4543794444325091No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA7370.39583646636732767No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG6840.36737061464756054No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG6600.35448041764238297No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG6540.3512578683910886No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG6380.3426644037209702No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA6080.32655165746449827No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT5900.3168840097106151No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG5290.2841214256557888No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG5160.2771392356113176No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG5100.2739166863600232No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA4650.24974756697531525No Hit
CATTTACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTC4640.24921047543343286No Hit
CCCCCACTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC4440.23846864459578493No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA4330.23256063763507853No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG4330.23256063763507853No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA4250.2282639053000193No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC4190.22504135604872497No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG3990.21429952521107698No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG3930.2110769759597826No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA3930.2110769759597826No Hit
TAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAGG3900.2094657013341354No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA3870.2078544267084882No Hit
GTAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAG3760.20194641974778182No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA3660.19657550432895782No Hit
AGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCTCCCTG3640.19550132124519304No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC3600.19335295507766345No Hit
GAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACAGAAGG3490.18744494811695706No Hit
GAGTGGGGGTCCCCGCATGCTGCTGTCCCCAGGGAGCTCAGCC3410.18314821578189788No Hit
GGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGCCAGCGT3400.18261112424001547No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC3300.17724020882119149No Hit
GAGCAGCAGGGAGAGGCAGAGGCGTGCGGCAGCCATCCGGAGT3220.17294347648613229No Hit
CGCTTGGTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGC3180.1707951103186027No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC3160.1697209272348379No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA3130.1681096526091907No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA3060.1643500118160139No Hit
TAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAGGCAG3020.16220164564848433No Hit
TGCCACACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGT3020.16220164564848433No Hit
GGACAATGCCACACCAGAGCAGATGGCCCAGTATGCAGCTGAT3010.16166455410660194No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG3000.16112746256471952No Hit
GCTCTGGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGG2880.15468236406213073No Hit
GTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCT2860.15360818097836595No Hit
GAGCAAGCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTG2860.15360818097836595No Hit
CAGGTGGACAGGAGCAGCAGGGAGAGGCAGAGGCGTGCGGCAG2840.15253399789460115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2830.15199690635271876No Hit
GGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGGGGCTC2810.15092272326895395No Hit
GCTCTCCCCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCC2780.14931144864330675No Hit
CCCATTTACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCC2710.14555180785012997No Hit
CCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTCTG2700.14501471630824758No Hit
GATCAGCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTC2680.14394053322448278No Hit
ACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAGTATGC2590.1391067093475412No Hit
GCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTGTTACT2590.1391067093475412No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC2580.1385696178056588No Hit
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG2560.13749543472189402No Hit
GTCTCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCT2560.13749543472189402No Hit
CTTATAATGCCACCTTCTGTCTCCTACGACTCCATGAGCAGCG2530.13588416009624682No Hit
ATGCCACACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCG2530.13588416009624682No Hit
CCACTGGAGCCAGTGTACCCAGGGGACAATGCCACACCAGAGC2530.13588416009624682No Hit
GAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCAT2500.13427288547059962No Hit
GCAGTGGCTGTAGTAACAGAGCCACGCAGGTGGACAGGAGCAG2500.13427288547059962No Hit
CTTCTGTCTCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCT2470.13266161084495243No Hit
GCCTGAGGCAGGTGCTCGCTTGGTCTAGTGCCCATTTACTCTG2410.12943906159365803No Hit
TCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTG2370.12729069542612842No Hit
GCATTGTCCCCTGGGTACACTGGCTCCAGTGGGGCTCCCTGGG2360.12675360388424603No Hit
GTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAGT2290.12299396309106923No Hit
GTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGGAG2280.12245687154918686No Hit
GGTGGACAGGAGCAGCAGGGAGAGGCAGAGGCGTGCGGCAGCC2280.12245687154918686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.12138268846542204No Hit
GGGAGAGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTA2240.12030850538165726No Hit
GACTTGCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTT2220.11923432229789246No Hit
GTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTC2210.11869723075601006No Hit
ATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTGT2190.11762304767224527No Hit
CAGCTGATCTCCGTAGATACATCAACATGCTGACCAGGCCTAG2180.11708595613036286No Hit
GCAGGGAGAGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGA2170.11654886458848045No Hit
CATCAACATGCTGACCAGGCCTAGGTATGGGAAAAGACACAAA2160.11601177304659807No Hit
GGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGCTGCAT2160.11601177304659807No Hit
ATGTTGATGTATCTACGGAGATCAGCTGCATACTGGGCCATCT2160.11601177304659807No Hit
TCAACATGCTGACCAGGCCTAGGTATGGGAAAAGACACAAAGA2120.11386340687906847No Hit
GTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAGTAT2110.11332631533718608No Hit
GACCAGGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTG2110.11332631533718608No Hit
CCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGGC2110.11332631533718608No Hit
AGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTTCTCGG2100.11278922379530368No Hit
CCCGCATGCTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTA2090.11225213225342127No Hit
CTCCAGTGGGGCTCCCTGGGCACCCAGCAGTGGCTGTAGTAAC2070.11117794916965648No Hit
GCCCAGCTCTCCCCTCTGCACCCTTGGCTCTGGCCAAAGCTTG2040.10956667454400928No Hit
GAGGACACGCTGGCCTTCTCGGAGTGGGGGTCCCCGCATGCTG2040.10956667454400928No Hit
CATTATAAGTCCAGCGGGCTGAGCTCCCTGGGGACAGCAGCAT2040.10956667454400928No Hit
GGTCAGCATGTTGATGTATCTACGGAGATCAGCTGCATACTGG2030.10902958300212688No Hit
CCCATACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGA2010.10795539991836209No Hit
CTGCTGCTCCTGTCCACCTGCGTGGCTCTGTTACTACAGCCAC2000.10741830837647968No Hit
GGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTG1980.10634412529271489No Hit
GTCCAGCGGGCTGAGCTCCCTGGGGACAGCAGCATGCGGGGAC1980.10634412529271489No Hit
CCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATACA1980.10634412529271489No Hit
ATGCTGACCAGGCCTAGGTATGGGAAAAGACACAAAGAGGACA1970.10580703375083249No Hit
GCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGG1940.1041957591251853No Hit
GCTCTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCAC1930.1036586675833029No Hit
GACACAAAGAGGACACGCTGGCCTTCTCGGAGTGGGGGTCCCC1880.10097320987389091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTA250.005484664329.64
GGGTCAC250.005484664329.635
GGTATCA2200.026.909091
TACTGTC450.00381395420.55555737
TGATTCC450.00381395420.55555717
GTATCAA2900.020.4137942
ATAAAGC555.120874E-420.18181830
TCTTATA1750.020.08571437
CGGCTGT656.862498E-519.92307733
GAGTCTG953.5736575E-617.52631634
AGTCTGT852.7033204E-517.41176435
CTCAATA752.0555654E-417.26666626
CTTTGAC752.0555654E-417.2666662
AATAAAG650.001573219317.07692329
TATCAAC3600.016.9583343
GCTTTGA904.413841E-516.4444451
ATCAACG3650.016.2191774
AGAGTCT803.363556E-416.187533
TTGACTT803.363556E-416.18754
GTCTGTC1151.2284981E-616.08695636
Produced by FastQC (version 0.11.5)