FastQCFastQC Report
Fri 10 Feb 2017
ERR1632143.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632143.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979619
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27720.2829671535566378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22310.22774160158183948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18880.19272798914680095No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10160.10371379076967678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11200.022.4642871
CTTATAC9600.020.42708237
TCGGGTC801.6162734E-518.537
TAAGCTA1352.2178938E-816.44444533
TCTTATA17050.015.95014737
TCTAATA1059.340198E-615.8571442
GTATTAT700.002592126615.8571431
TCAATAC700.002592126615.8571433
TACCGTC700.002592126615.8571437
CCTAAAC1302.5876216E-715.6538463
GTATCAA16450.015.1823712
CACGCAT1101.451354E-515.1363649
CTAATCA1101.451354E-515.1363644
ATTGCCG2151.2732926E-1114.62790711
CTAAGTC1803.3269316E-914.3888894
GTCCTAA2201.8189894E-1114.2954541
CGTCACT1458.905299E-714.03448420
CTATAAG800.00629774613.8751
GTGCTAG2304.0017767E-1113.6739131
TCTAGCG950.001244843813.63157928
Produced by FastQC (version 0.11.5)