##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632143.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	979619
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59367876694919	34.0	31.0	34.0	31.0	34.0
2	32.77097626730392	34.0	31.0	34.0	31.0	34.0
3	32.849567025547685	34.0	31.0	34.0	31.0	34.0
4	36.30159684530415	37.0	37.0	37.0	35.0	37.0
5	36.15009712959834	37.0	35.0	37.0	35.0	37.0
6	36.17776604986224	37.0	36.0	37.0	35.0	37.0
7	36.16103403466041	37.0	36.0	37.0	35.0	37.0
8	36.14343739760049	37.0	36.0	37.0	35.0	37.0
9	37.89319316999772	39.0	38.0	39.0	35.0	39.0
10	37.85231299107102	39.0	38.0	39.0	35.0	39.0
11	37.92198701740166	39.0	38.0	39.0	35.0	39.0
12	37.889309006869	39.0	38.0	39.0	35.0	39.0
13	37.902758113103154	39.0	38.0	39.0	35.0	39.0
14	39.25697541595253	40.0	39.0	41.0	36.0	41.0
15	39.26820937527753	40.0	39.0	41.0	36.0	41.0
16	39.25233381549357	40.0	39.0	41.0	36.0	41.0
17	39.251109870265886	40.0	39.0	41.0	36.0	41.0
18	39.246655077126924	40.0	39.0	41.0	36.0	41.0
19	39.290536422833775	40.0	39.0	41.0	36.0	41.0
20	39.2646845355184	40.0	39.0	41.0	36.0	41.0
21	39.234980130030145	40.0	39.0	41.0	36.0	41.0
22	39.19216042155164	40.0	39.0	41.0	36.0	41.0
23	39.13867942536844	40.0	39.0	41.0	36.0	41.0
24	39.09150904586375	40.0	39.0	41.0	36.0	41.0
25	39.04308103456548	40.0	39.0	41.0	36.0	41.0
26	38.95277755943892	40.0	38.0	41.0	35.0	41.0
27	38.87070687685723	40.0	38.0	41.0	35.0	41.0
28	38.80208530050969	40.0	38.0	41.0	35.0	41.0
29	38.73909856791263	40.0	38.0	41.0	35.0	41.0
30	38.660931443755175	40.0	38.0	41.0	35.0	41.0
31	38.579471202579775	40.0	38.0	41.0	35.0	41.0
32	38.48934943074808	40.0	38.0	41.0	35.0	41.0
33	38.40786877347214	40.0	38.0	41.0	34.0	41.0
34	38.33674418319775	40.0	38.0	41.0	34.0	41.0
35	38.29780149221279	40.0	38.0	41.0	34.0	41.0
36	38.26375662374862	40.0	38.0	41.0	34.0	41.0
37	38.20596170552021	40.0	38.0	41.0	34.0	41.0
38	38.146104761136726	40.0	38.0	41.0	34.0	41.0
39	38.092557412626746	40.0	38.0	41.0	34.0	41.0
40	38.02686350509739	40.0	38.0	41.0	34.0	41.0
41	37.96320610359742	40.0	37.0	41.0	33.0	41.0
42	37.904665997699105	40.0	37.0	41.0	33.0	41.0
43	37.041442642496726	39.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	0.0
13	2.0
14	2.0
15	3.0
16	8.0
17	12.0
18	18.0
19	25.0
20	81.0
21	133.0
22	262.0
23	442.0
24	745.0
25	1255.0
26	1842.0
27	2745.0
28	3955.0
29	5669.0
30	7930.0
31	10530.0
32	13964.0
33	19405.0
34	27231.0
35	39006.0
36	62336.0
37	115133.0
38	274377.0
39	392504.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.58698432758042	19.260345093347517	13.384693436938239	25.76797714213383
2	18.582938877257384	20.90200373818801	35.14917534265873	25.365882041895883
3	19.80453625338014	22.437906982204307	30.500531329016688	27.257025435398862
4	15.271651529829455	16.189763571347637	34.02761685920751	34.510968039615406
5	15.370975858981911	35.446944169110644	33.72974595225286	15.452334019654579
6	33.81181867644462	34.692160931954156	16.018574568276033	15.477445823325189
7	28.627558264998942	30.425400079010313	21.312775681157675	19.634265974833074
8	26.414350885395237	33.48669227526212	20.558094524503915	19.540862314838726
9	26.18997794040336	14.869046027077873	20.467447038083172	38.47352899443559
10	17.30887212273343	26.88330871491876	32.88441730917837	22.923401853169448
11	35.147746215620565	21.905455079985177	21.6426998659683	21.304098838425958
12	21.572774721600947	25.9648904318924	28.630314438572547	23.832020407934102
13	30.102723609893232	19.6951059544578	25.04443053881152	25.157739896837445
14	22.910845951334142	21.38749860915315	24.902844881530473	30.798810557982236
15	25.5293129267603	27.603894983662013	22.42045121623815	24.446340873339533
16	24.939900104020033	26.484378110265318	24.111006421884426	24.464715363830226
17	23.59978726423232	26.699461729509128	25.499913741975195	24.200837264283358
18	23.22280396766498	25.153554596225675	27.11033575298152	24.51330568312783
19	24.429701751395186	25.57188049639707	26.79010921592987	23.20830853627788
20	24.91019467772675	24.861706438931872	26.602893573930274	23.625205309411108
21	25.042388928757	25.554935132944546	25.41120578510625	23.99147015319221
22	24.922750579562052	25.117418098260654	25.486745357123535	24.473085965053762
23	24.117029171545266	25.31514803204103	25.990002235563008	24.5778205608507
24	24.619061083952026	25.539827218541085	25.8109530337815	24.030158663725388
25	24.517593064242323	24.86272724395913	26.036244703297918	24.58343498850063
26	24.40520243074093	25.483887103047202	25.81789450796687	24.29301595824499
27	24.479517036725504	25.101595620338113	25.590867469904115	24.828019873032268
28	23.66113764637068	25.494299314325264	26.40720525020442	24.43735778909964
29	23.679512136861373	25.743375740976848	26.40914477975621	24.167967342405568
30	23.582025256757984	26.380562238992916	26.245713894891793	23.79169860935731
31	24.304959377063938	25.634353764065416	26.02266799643535	24.038018862435294
32	23.295689446611387	25.338320306159844	26.34850896113693	25.017481286091837
33	23.39378880973113	25.137221715789504	26.649850605184263	24.819138869295106
34	24.178685795191804	24.610996724236667	26.583804519920502	24.626512960651027
35	23.856825970096537	24.712158502438193	26.970485464246813	24.460530063218457
36	23.239034767598422	24.715629239530877	27.229157458154653	24.816178534716048
37	23.89867897621422	23.352242045121624	27.39871317318264	25.350365805481516
38	22.837245908868653	23.536191111033983	28.75179023681656	24.874772743280808
39	22.639618055591	23.09408045372742	29.209927533051115	25.056373957630466
40	22.203632228447997	22.999349747197634	29.998091094599022	24.798926929755343
41	21.40515853612476	23.313043132074817	30.541261449604384	24.740536882196036
42	21.783979281741168	23.087955623563854	30.136410175792832	24.991654918902146
43	20.57942934957366	23.22801007330401	30.532074204359045	25.660486372763287
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	174.0
1	160.5
2	147.0
3	310.0
4	473.0
5	473.0
6	622.5
7	772.0
8	771.5
9	771.0
10	1164.0
11	1557.0
12	1557.0
13	2545.5
14	3534.0
15	5131.0
16	6728.0
17	6551.5
18	6375.0
19	6375.0
20	7391.0
21	8407.0
22	8887.0
23	9367.0
24	10840.0
25	12313.0
26	12313.0
27	13858.5
28	15404.0
29	19655.5
30	23907.0
31	26549.0
32	29191.0
33	29191.0
34	32648.5
35	36106.0
36	39997.0
37	43888.0
38	48011.0
39	52134.0
40	52134.0
41	56112.0
42	60090.0
43	59463.5
44	58837.0
45	61970.0
46	65103.0
47	65103.0
48	66689.0
49	68275.0
50	70975.0
51	73675.0
52	76672.5
53	79670.0
54	79670.0
55	72612.0
56	65554.0
57	62228.0
58	58902.0
59	53868.0
60	48834.0
61	48834.0
62	44950.5
63	41067.0
64	36664.5
65	32262.0
66	28122.0
67	23982.0
68	23982.0
69	20812.5
70	17643.0
71	15346.5
72	13050.0
73	10667.5
74	8285.0
75	8285.0
76	6573.5
77	4862.0
78	4088.5
79	3315.0
80	2657.5
81	2000.0
82	2000.0
83	1649.5
84	1299.0
85	1049.0
86	799.0
87	668.0
88	537.0
89	537.0
90	399.5
91	262.0
92	156.5
93	51.0
94	31.0
95	11.0
96	11.0
97	7.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	979619.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.725640457905094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.39070471686271	56.69814844806598
2	5.3778389841293555	6.746567891180072
3	1.3904609952502844	2.616526693764307
4	0.6820870517030717	1.711373886644776
5	0.3900304215032743	1.223245399342978
6	0.2625488228255377	0.9881125837920545
7	0.1840621346708979	0.8081790696896877
8	0.14768548268022355	0.7410933189961498
9	0.11073570055704467	0.6251370965095884
>10	0.8397833239195166	10.582328700570457
>50	0.13190332684959233	5.855919632970574
>100	0.09020513721438195	10.083603102526215
>500	0.0013026012227318117	0.5121975965310905
>1k	6.513006113659059E-4	0.8075665794160186
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2772	0.2829671535566378	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2231	0.22774160158183948	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1888	0.19272798914680095	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1016	0.10371379076967678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.0208050272605982E-4	0.0	0.0	0.0	0.0
6	1.0208050272605982E-4	0.0	0.0	0.0	0.0
7	1.0208050272605982E-4	0.0	0.0	0.0	0.0
8	1.0208050272605982E-4	0.0	0.0	0.0	0.0
9	1.0208050272605982E-4	0.0	0.0	0.0	0.0
10	1.0208050272605982E-4	0.0	0.0	1.0208050272605982E-4	0.0
11	1.0208050272605982E-4	0.0	0.0	2.0416100545211964E-4	0.0
12	1.0208050272605982E-4	0.0	0.0	3.0624150817817945E-4	0.0
13	1.0208050272605982E-4	0.0	0.0	3.0624150817817945E-4	0.0
14	1.0208050272605982E-4	0.0	0.0	4.083220109042393E-4	0.0
15	1.0208050272605982E-4	0.0	0.0	5.104025136302992E-4	0.0
16	1.0208050272605982E-4	0.0	0.0	9.187245245345384E-4	0.0
17	1.0208050272605982E-4	0.0	0.0	0.0021436905572472563	0.0
18	1.0208050272605982E-4	0.0	0.0	0.002245771059973316	0.0
19	2.0416100545211964E-4	0.0	0.0	0.002347851562699376	0.0
20	2.0416100545211964E-4	0.0	0.0	0.002347851562699376	0.0
21	2.0416100545211964E-4	0.0	0.0	0.002858254076329675	0.0
22	2.0416100545211964E-4	0.0	0.0	0.004491542119946632	0.0
23	2.0416100545211964E-4	0.0	0.0	0.00581858865538541	0.0
24	2.0416100545211964E-4	0.0	0.0	0.007962279212632666	0.0
25	2.0416100545211964E-4	0.0	0.0	0.009697647758975683	0.0
26	2.0416100545211964E-4	0.0	0.0	0.015516236414361093	0.0
27	2.0416100545211964E-4	0.0	0.0	0.04052595958224575	0.0
28	2.0416100545211964E-4	0.0	0.0	0.12504861583942328	0.0
29	2.0416100545211964E-4	0.0	0.0	0.24050166442259696	0.0
30	2.0416100545211964E-4	0.0	0.0	0.3950515455498515	0.0
31	2.0416100545211964E-4	0.0	0.0	0.9079039912455761	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1120	0.0	22.464287	1
CTTATAC	960	0.0	20.427082	37
TCGGGTC	80	1.6162734E-5	18.5	37
TAAGCTA	135	2.2178938E-8	16.444445	33
TCTTATA	1705	0.0	15.950147	37
TCTAATA	105	9.340198E-6	15.857144	2
GTATTAT	70	0.0025921266	15.857143	1
TCAATAC	70	0.0025921266	15.857143	3
TACCGTC	70	0.0025921266	15.857143	7
CCTAAAC	130	2.5876216E-7	15.653846	3
GTATCAA	1645	0.0	15.182371	2
CACGCAT	110	1.451354E-5	15.136364	9
CTAATCA	110	1.451354E-5	15.136364	4
ATTGCCG	215	1.2732926E-11	14.627907	11
CTAAGTC	180	3.3269316E-9	14.388889	4
GTCCTAA	220	1.8189894E-11	14.295454	1
CGTCACT	145	8.905299E-7	14.034484	20
CTATAAG	80	0.006297746	13.875	1
GTGCTAG	230	4.0017767E-11	13.673913	1
TCTAGCG	95	0.0012448438	13.631579	28
>>END_MODULE