FastQCFastQC Report
Fri 10 Feb 2017
ERR1632142.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632142.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254773
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG200.001839248637.05
CGACGGT250.005488415229.67
CTACGAC250.005488415229.64
ACGACGG250.005488415229.66
TACATGC451.3192664E-424.6666667
CTAATAC450.00381776120.5555553
TAATACA656.875212E-519.9230774
GGTATCA1052.2388122E-819.380951
GTACAAG500.00702022718.51
CATGCCG500.00702022718.59
CAACAGT500.00702022718.56
ATACATG500.00702022718.56
GGACTGA752.05932E-417.2666666
TTTGAAC650.001575374317.0769233
CTTATAC1951.8189894E-1216.12820637
TTGAACT700.002585073215.8571434
TGGACAG957.025256E-515.5789485
GTATCAA1353.936657E-715.0740752
CTCCAAG1001.08806715E-414.81
TCTTATA3400.014.69117537
Produced by FastQC (version 0.11.5)