##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632142.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254773
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.732671829432476	34.0	31.0	34.0	31.0	34.0
2	32.905162634973095	34.0	31.0	34.0	31.0	34.0
3	32.990430697130385	34.0	31.0	34.0	31.0	34.0
4	36.39252589560118	37.0	37.0	37.0	35.0	37.0
5	36.24926895707159	37.0	37.0	37.0	35.0	37.0
6	36.273914425782955	37.0	37.0	37.0	35.0	37.0
7	36.255321403759424	37.0	37.0	37.0	35.0	37.0
8	36.238141404308934	37.0	36.0	37.0	35.0	37.0
9	38.02982655147916	39.0	38.0	39.0	35.0	39.0
10	37.99719358016744	39.0	38.0	39.0	35.0	39.0
11	38.06556032232615	39.0	38.0	39.0	35.0	39.0
12	38.02382905566916	39.0	38.0	39.0	35.0	39.0
13	38.046327515082055	39.0	38.0	39.0	35.0	39.0
14	39.4381272740832	40.0	39.0	41.0	37.0	41.0
15	39.43851585529079	40.0	39.0	41.0	37.0	41.0
16	39.4400309294941	40.0	39.0	41.0	37.0	41.0
17	39.41419224172106	40.0	39.0	41.0	37.0	41.0
18	39.40735085742995	40.0	39.0	41.0	37.0	41.0
19	39.4308855333964	40.0	39.0	41.0	37.0	41.0
20	39.394402075573154	40.0	39.0	41.0	37.0	41.0
21	39.36991360936991	40.0	39.0	41.0	37.0	41.0
22	39.32539554819388	40.0	39.0	41.0	36.0	41.0
23	39.279896221342135	40.0	39.0	41.0	36.0	41.0
24	39.22215462392012	40.0	39.0	41.0	36.0	41.0
25	39.17254575641846	40.0	39.0	41.0	36.0	41.0
26	39.08547609048055	40.0	39.0	41.0	36.0	41.0
27	39.008234781550634	40.0	39.0	41.0	35.0	41.0
28	38.93740310001452	40.0	39.0	41.0	35.0	41.0
29	38.86498569314645	40.0	38.0	41.0	35.0	41.0
30	38.80002983047654	40.0	38.0	41.0	35.0	41.0
31	38.72593642183434	40.0	38.0	41.0	35.0	41.0
32	38.644887802082636	40.0	38.0	41.0	35.0	41.0
33	38.559851318624816	40.0	38.0	41.0	35.0	41.0
34	38.49111954563474	40.0	38.0	41.0	34.0	41.0
35	38.463871760351374	40.0	38.0	41.0	34.0	41.0
36	38.41754817033202	40.0	38.0	41.0	34.0	41.0
37	38.36276607018797	40.0	38.0	41.0	34.0	41.0
38	38.31426014530582	40.0	38.0	41.0	34.0	41.0
39	38.264706228682	40.0	38.0	41.0	34.0	41.0
40	38.196374812087626	40.0	38.0	41.0	34.0	41.0
41	38.15148386995482	40.0	37.0	41.0	34.0	41.0
42	38.09777723699136	40.0	37.0	41.0	34.0	41.0
43	37.191970891735	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	4.0
19	7.0
20	19.0
21	30.0
22	78.0
23	98.0
24	152.0
25	279.0
26	431.0
27	559.0
28	809.0
29	1198.0
30	1757.0
31	2351.0
32	3187.0
33	4436.0
34	6595.0
35	9574.0
36	15697.0
37	28322.0
38	66552.0
39	112635.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46182680268317	17.136038748219004	11.768515502035145	27.633618947062676
2	20.552020818532576	19.227312156311697	31.969635714930543	28.251031310225184
3	21.180030850992846	20.419746205445634	27.86441263399183	30.535810309569694
4	16.46210548213508	14.823391803684064	31.606174908644164	37.108327805536696
5	17.78092655030164	33.119286580603124	32.050884512880096	17.04890235621514
6	37.76538330199825	31.891134460873012	14.427352976963807	15.916129260164933
7	31.925282506388044	27.41460044824216	19.61000576984217	21.050111275527627
8	28.90808680668674	30.924391517154486	18.75905217585851	21.408469500300267
9	27.536277392031337	13.417434343513637	18.147134900480037	40.89915336397499
10	18.84108598634863	24.37856444756705	31.249386708952677	25.530962857131644
11	39.10932477146322	19.744635420550843	19.27402040247593	21.872019405510006
12	24.06338191252605	23.01382014577683	26.560506804096196	26.362291137600923
13	33.014879912706604	17.47634168455841	21.846113991671018	27.662664411063965
14	24.74241776012372	18.613824855852073	22.20604224152481	34.4377151424994
15	27.68228972457835	25.06584292684076	19.747775470713144	27.504091877867747
16	27.803574162097238	23.472267469472825	21.602367597822376	27.12179077060756
17	26.621345275990784	23.79961769889274	22.767326208036174	26.811710817080304
18	26.863914151028563	22.66684460284253	23.54684366082748	26.92239758530143
19	27.390657565754612	22.905095908907143	23.058958366859912	26.64528815847833
20	27.526857241544434	22.817959516903283	22.97496202501835	26.680221216533933
21	28.002967347403374	22.744168338089203	22.388557657208572	26.864306657298847
22	28.10815902784047	22.68332986619461	22.276693370176588	26.93181773578833
23	27.58965824479046	22.5373175336476	22.48158164326675	27.391442578295187
24	27.19872199958394	23.060528391941062	22.22606006130948	27.514689547165517
25	27.513512028354654	22.299066227582987	22.545560165323643	27.64186157873872
26	27.2477852833699	22.77635385225279	22.624061419381174	27.351799444996132
27	26.99618876411551	22.72101046814223	22.540850090080188	27.741950677662075
28	27.02091665914363	22.719440443061078	22.98477468177554	27.274868216019748
29	26.707696655454072	22.927076260043254	23.356085613467677	27.009141471034997
30	26.573067004745397	23.300742229357112	22.99851240123561	27.12767836466188
31	27.13670600887849	22.957299242855406	22.669592146734544	27.236402601531562
32	26.39290662668336	22.930608816475843	23.14609475886377	27.530389797977023
33	26.289284971327415	22.560082897324286	23.455389699850453	27.695242431497842
34	26.881184426921216	22.115373293088357	23.516620678015332	27.48682160197509
35	26.415671990360046	22.276693370176588	24.13795810388071	27.169676535582653
36	26.147982714023854	22.246077881094152	24.009216047226353	27.59672335765564
37	26.06948145996632	21.24165433542801	24.82955415212758	27.859310052478087
38	25.191052427062523	21.32211812083698	25.88304098157968	27.60378847052082
39	24.941418439159566	20.994767891417066	26.47376291836262	27.590050751060748
40	24.738100191150554	20.882511098114794	27.242682701856165	27.13670600887849
41	24.176423718368902	20.70117320124189	27.71055017603906	27.411852904350148
42	23.635157571642207	20.936284457144204	28.086963689244936	27.341594281968657
43	22.97299949366691	20.551235805992	28.479077453262313	27.99668724707877
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	8.0
4	14.0
5	14.0
6	16.5
7	19.0
8	22.0
9	25.0
10	33.5
11	42.0
12	42.0
13	88.0
14	134.0
15	199.0
16	264.0
17	279.5
18	295.0
19	295.0
20	371.0
21	447.0
22	502.5
23	558.0
24	755.0
25	952.0
26	952.0
27	1209.5
28	1467.0
29	1906.5
30	2346.0
31	2871.5
32	3397.0
33	3397.0
34	4268.5
35	5140.0
36	6099.5
37	7059.0
38	8120.5
39	9182.0
40	9182.0
41	10259.5
42	11337.0
43	12485.0
44	13633.0
45	14835.5
46	16038.0
47	16038.0
48	16821.5
49	17605.0
50	18415.0
51	19225.0
52	19918.0
53	20611.0
54	20611.0
55	20821.0
56	21031.0
57	20604.0
58	20177.0
59	19759.0
60	19341.0
61	19341.0
62	18055.5
63	16770.0
64	15194.0
65	13618.0
66	12206.5
67	10795.0
68	10795.0
69	9368.0
70	7941.0
71	6791.0
72	5641.0
73	4631.0
74	3621.0
75	3621.0
76	2978.5
77	2336.0
78	1938.5
79	1541.0
80	1280.5
81	1020.0
82	1020.0
83	780.0
84	540.0
85	437.5
86	335.0
87	251.0
88	167.0
89	167.0
90	121.0
91	75.0
92	47.5
93	20.0
94	13.5
95	7.0
96	7.0
97	5.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	254773.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.07632632133773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.03919913056062	76.46278541325037
2	5.025326502915729	8.349713288549882
3	1.2137823798545992	3.0250974321567177
4	0.5403616314820956	1.7956503691414807
5	0.31111677073465516	1.2923219184796515
6	0.20003976257449335	0.9971141155728919
7	0.13114638957625807	0.762661217941175
8	0.1011158895283147	0.6720269307781287
9	0.06841250587846542	0.5115113696537846
>10	0.3520059088575769	5.108726509675727
>50	0.01607476630446609	0.8923831501598475
>100	0.0014183617327469993	0.13000828464036449
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.9250627028766783E-4
2	0.0	0.0	0.0	0.0	3.9250627028766783E-4
3	0.0	0.0	0.0	0.0	3.9250627028766783E-4
4	0.0	0.0	0.0	0.0	3.9250627028766783E-4
5	0.0	0.0	0.0	0.0	3.9250627028766783E-4
6	0.0	0.0	0.0	0.0	3.9250627028766783E-4
7	0.0	0.0	0.0	0.0	3.9250627028766783E-4
8	0.0	0.0	0.0	0.0	3.9250627028766783E-4
9	0.0	0.0	0.0	0.0	3.9250627028766783E-4
10	0.0	0.0	0.0	0.0	3.9250627028766783E-4
11	0.0	0.0	0.0	0.0	3.9250627028766783E-4
12	0.0	0.0	0.0	0.0	3.9250627028766783E-4
13	0.0	0.0	0.0	0.0	3.9250627028766783E-4
14	0.0	0.0	0.0	0.0	3.9250627028766783E-4
15	0.0	0.0	0.0	0.0	3.9250627028766783E-4
16	0.0	0.0	0.0	0.0	3.9250627028766783E-4
17	0.0	0.0	0.0	3.9250627028766783E-4	3.9250627028766783E-4
18	0.0	0.0	0.0	3.9250627028766783E-4	3.9250627028766783E-4
19	0.0	0.0	0.0	3.9250627028766783E-4	3.9250627028766783E-4
20	0.0	0.0	0.0	3.9250627028766783E-4	3.9250627028766783E-4
21	0.0	0.0	0.0	7.850125405753357E-4	3.9250627028766783E-4
22	0.0	0.0	0.0	7.850125405753357E-4	3.9250627028766783E-4
23	0.0	0.0	0.0	0.002355037621726007	3.9250627028766783E-4
24	0.0	0.0	0.0	0.005887594054315018	3.9250627028766783E-4
25	0.0	0.0	0.0	0.008242631676041026	3.9250627028766783E-4
26	0.0	0.0	0.0	0.01256020064920537	3.9250627028766783E-4
27	0.0	0.0	0.0	0.041213158380205124	3.9250627028766783E-4
28	0.0	0.0	0.0	0.11814438735658803	3.9250627028766783E-4
29	0.0	0.0	0.0	0.22137353644224467	3.9250627028766783E-4
30	0.0	0.0	0.0	0.3815160947196131	3.9250627028766783E-4
31	0.0	0.0	0.0	0.772844846196418	3.9250627028766783E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACG	20	0.0018392486	37.0	5
CGACGGT	25	0.0054884152	29.6	7
CTACGAC	25	0.0054884152	29.6	4
ACGACGG	25	0.0054884152	29.6	6
TACATGC	45	1.3192664E-4	24.666666	7
CTAATAC	45	0.003817761	20.555555	3
TAATACA	65	6.875212E-5	19.923077	4
GGTATCA	105	2.2388122E-8	19.38095	1
GTACAAG	50	0.007020227	18.5	1
CATGCCG	50	0.007020227	18.5	9
CAACAGT	50	0.007020227	18.5	6
ATACATG	50	0.007020227	18.5	6
GGACTGA	75	2.05932E-4	17.266666	6
TTTGAAC	65	0.0015753743	17.076923	3
CTTATAC	195	1.8189894E-12	16.128206	37
TTGAACT	70	0.0025850732	15.857143	4
TGGACAG	95	7.025256E-5	15.578948	5
GTATCAA	135	3.936657E-7	15.074075	2
CTCCAAG	100	1.08806715E-4	14.8	1
TCTTATA	340	0.0	14.691175	37
>>END_MODULE