FastQCFastQC Report
Fri 10 Feb 2017
ERR1632141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569559
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46020.29320337750922393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34920.22248287576319206No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31460.2004384671108254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.021.4329261
CTTATAC21000.017.44285637
GTCCTAA2400.016.18751
GTATTAA1751.3096724E-1015.8571431
AGACCGT1403.475361E-815.8571436
TCTTATA36750.015.75646137
CTATCGC957.062306E-515.57894830
ACCGTCG2150.015.4883728
ATACACA6250.015.39237
GACGGAC3700.015.0000017
CGTCGTA1853.0559022E-1015.00000110
CAAGACG3700.015.0000014
TACCGTC2109.094947E-1214.97619157
AAGACGG3750.014.7999995
GTATAAA1803.3323886E-914.3888881
GCGGTAT1956.730261E-1014.2307724
CTTAGGG1304.4461667E-614.2307693
CGCATCG2353.6379788E-1214.17021413
GATTTAT1854.9003575E-914.0000011
GTATCAA25150.013.9025842
Produced by FastQC (version 0.11.5)