##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632141.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1569559
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.764798264990354	34.0	31.0	34.0	31.0	34.0
2	32.932576602727266	34.0	31.0	34.0	31.0	34.0
3	33.017571814758156	34.0	33.0	34.0	31.0	34.0
4	36.409611234748105	37.0	37.0	37.0	35.0	37.0
5	36.27969193894591	37.0	37.0	37.0	35.0	37.0
6	36.30653259928426	37.0	37.0	37.0	35.0	37.0
7	36.28141917570477	37.0	37.0	37.0	35.0	37.0
8	36.27223060745088	37.0	37.0	37.0	35.0	37.0
9	38.059475941968415	39.0	38.0	39.0	35.0	39.0
10	38.0277268965359	39.0	38.0	39.0	35.0	39.0
11	38.09513309152443	39.0	38.0	39.0	37.0	39.0
12	38.05730654279323	39.0	38.0	39.0	35.0	39.0
13	38.08011995726188	39.0	38.0	39.0	37.0	39.0
14	39.471176298565396	40.0	39.0	41.0	37.0	41.0
15	39.4797971914404	40.0	39.0	41.0	37.0	41.0
16	39.47158214504839	40.0	39.0	41.0	37.0	41.0
17	39.468892854617124	40.0	39.0	41.0	37.0	41.0
18	39.46263886862488	40.0	39.0	41.0	37.0	41.0
19	39.4862295714911	41.0	39.0	41.0	37.0	41.0
20	39.45699142243139	40.0	39.0	41.0	37.0	41.0
21	39.43308534435469	40.0	39.0	41.0	37.0	41.0
22	39.390543458385444	40.0	39.0	41.0	37.0	41.0
23	39.33602304851235	40.0	39.0	41.0	36.0	41.0
24	39.28526547902946	40.0	39.0	41.0	36.0	41.0
25	39.22884262394724	40.0	39.0	41.0	36.0	41.0
26	39.141873609083824	40.0	39.0	41.0	36.0	41.0
27	39.06430596110118	40.0	39.0	41.0	35.0	41.0
28	38.989334583790736	40.0	39.0	41.0	35.0	41.0
29	38.91752141843664	40.0	38.0	41.0	35.0	41.0
30	38.84067435502584	40.0	38.0	41.0	35.0	41.0
31	38.762010857826944	40.0	38.0	41.0	35.0	41.0
32	38.679009199399324	40.0	38.0	41.0	35.0	41.0
33	38.59086596935827	40.0	38.0	41.0	35.0	41.0
34	38.506311645500425	40.0	38.0	41.0	35.0	41.0
35	38.47671607120217	40.0	38.0	41.0	35.0	41.0
36	38.43065536242983	40.0	38.0	41.0	35.0	41.0
37	38.371820364828594	40.0	38.0	41.0	34.0	41.0
38	38.3233774582542	40.0	38.0	41.0	34.0	41.0
39	38.258534403612735	40.0	38.0	41.0	34.0	41.0
40	38.17445600961799	40.0	38.0	41.0	34.0	41.0
41	38.11202000052244	40.0	37.0	41.0	34.0	41.0
42	38.04494638302861	40.0	37.0	41.0	34.0	41.0
43	37.17330218233275	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	3.0
12	0.0
13	1.0
14	1.0
15	5.0
16	3.0
17	8.0
18	20.0
19	43.0
20	77.0
21	183.0
22	353.0
23	630.0
24	1045.0
25	1622.0
26	2406.0
27	3665.0
28	5501.0
29	7534.0
30	10706.0
31	14389.0
32	19522.0
33	26484.0
34	39209.0
35	58064.0
36	92890.0
37	171842.0
38	393520.0
39	719829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.180791547179815	17.642662684231684	12.261660759487219	26.91488500910128
2	19.360916028005317	19.821937244792963	33.35669446003623	27.460452267165493
3	20.09908515704093	21.200477331530703	29.121109814922537	29.579327696505835
4	15.258553517261856	15.296143693865602	32.92466227774808	36.52064051112446
5	16.61250070879782	34.0971572269663	32.4844112263381	16.80593083789778
6	35.771767738581346	33.22436429595829	15.395662093619928	15.608205871840433
7	30.737041423737494	29.09982995223499	20.180509302294467	19.982619321733047
8	28.424863289624664	31.701452446196672	19.405641966947403	20.46804229723126
9	27.426939669040795	13.803431409714447	19.011199961263003	39.75842895998175
10	18.204476544048358	25.40503415290537	31.442653637104435	24.947835665941835
11	37.7667230094568	20.49531110330991	20.452177968461204	21.28578791877209
12	23.163831369193513	24.332121315605214	27.68981605661208	24.814231258589196
13	31.762042713908812	18.307053127661973	23.37949704343704	26.55140711499217
14	23.797894822685866	19.474833376763794	23.97514206219709	32.75212973835326
15	26.572941826334656	26.041518668619656	21.207421957377836	26.17811754766785
16	26.34829273700447	24.529183038038074	23.041312878330793	26.081211346626667
17	25.160315731998605	24.951658395765943	24.216292601934683	25.671733270300766
18	25.407200366472367	23.609115681538572	25.265058529179214	25.71862542280985
19	25.87981719705981	24.095621763820283	25.041874819614936	24.98268621950497
20	26.12383478416549	23.652121392059808	24.650936982935974	25.573106840838733
21	26.806319482096562	23.794326941516694	24.234323144271734	25.16503043211501
22	26.941962678688725	23.64237343100833	24.002474580439472	25.41318930986347
23	25.985961661842595	23.605356663878197	24.329572829055802	26.079108845223402
24	25.614710883757795	24.14640035831721	24.220497604741205	26.01839115318379
25	25.845157779987883	23.285776450582617	24.492548543890354	26.376517225539146
26	25.593239884579045	23.976543729799264	24.703053532871337	25.727162852750357
27	25.823623068645396	23.639761232295186	24.44145138857475	26.095164310484662
28	25.38802300518808	23.944623935767943	24.928212319511402	25.73914073953257
29	25.252634657250862	24.115882231888065	25.048500884643392	25.58298222621768
30	25.12176987293883	24.336326318411732	24.87157220595084	25.670331602698592
31	25.664533795798693	23.982532673190367	24.3757641477638	25.97716938324714
32	24.771862669705314	23.78279503988063	24.902281468871195	26.54306082154287
33	24.78600677005452	23.451300651966573	25.603115269957993	26.159577308020914
34	25.55036159838528	23.005442930147897	25.46256623675822	25.981629234708603
35	25.11788343095099	23.086867075401436	25.982967190147043	25.812282303500538
36	24.510387949736202	23.022517790028918	26.01004485973449	26.45704940050039
37	24.421190920506973	22.043070696928247	26.620025115334943	26.91571326722984
38	23.7481356228087	21.735340946087405	27.823993873438337	26.692529557665562
39	23.692514903867902	21.33586567946793	28.394026602376847	26.577592814287325
40	23.354075890106714	21.48176653442145	29.0804614544595	26.083696121012334
41	22.47172613453843	21.44060847664854	29.918849817050525	26.168815571762515
42	22.248860985792824	21.78662923789421	29.854245683023066	26.1102640932899
43	21.37020653572118	21.257499718073674	30.24575692917565	27.126536817029496
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	169.0
1	179.5
2	190.0
3	432.0
4	674.0
5	674.0
6	884.0
7	1094.0
8	1075.0
9	1056.0
10	1573.5
11	2091.0
12	2091.0
13	3377.5
14	4664.0
15	7072.5
16	9481.0
17	8806.5
18	8132.0
19	8132.0
20	9005.0
21	9878.0
22	9127.0
23	8376.0
24	9988.0
25	11600.0
26	11600.0
27	13527.0
28	15454.0
29	18936.5
30	22419.0
31	26289.5
32	30160.0
33	30160.0
34	36187.0
35	42214.0
36	48337.5
37	54461.0
38	60908.5
39	67356.0
40	67356.0
41	73277.0
42	79198.0
43	84685.0
44	90172.0
45	95539.5
46	100907.0
47	100907.0
48	105974.0
49	111041.0
50	113752.0
51	116463.0
52	118138.5
53	119814.0
54	119814.0
55	118501.0
56	117188.0
57	114186.0
58	111184.0
59	107376.5
60	103569.0
61	103569.0
62	95813.5
63	88058.0
64	79699.5
65	71341.0
66	63176.0
67	55011.0
68	55011.0
69	47461.0
70	39911.0
71	34317.5
72	28724.0
73	23281.0
74	17838.0
75	17838.0
76	14520.0
77	11202.0
78	9229.0
79	7256.0
80	6068.5
81	4881.0
82	4881.0
83	3801.5
84	2722.0
85	2295.0
86	1868.0
87	1481.5
88	1095.0
89	1095.0
90	800.5
91	506.0
92	305.0
93	104.0
94	65.0
95	26.0
96	26.0
97	17.0
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1569559.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.27746272416967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.42781666834645	58.81921676960218
2	7.437295839614752	10.007247872366074
3	2.0154441183423075	4.067818996332647
4	0.9263449584619554	2.4928855365058684
5	0.48293952121357075	1.6245472818237179
6	0.3233095884099929	1.3050869269571965
7	0.23139068684756023	1.0897164816374725
8	0.16199270020685363	0.8718766279803349
9	0.127869548401473	0.7742464898522858
>10	0.7619804093825049	9.672026045618088
>50	0.06161685976876618	2.8335468469600182
>100	0.04019788543389884	5.055662382171019
>500	0.001422012292116624	0.5761847797805838
>1k	3.792032778977664E-4	0.8099369624124011
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4602	0.29320337750922393	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3492	0.22248287576319206	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3146	0.2004384671108254	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.2742432747032766E-4	0.0
10	0.0	0.0	0.0	1.911364912054915E-4	0.0
11	0.0	0.0	0.0	2.548486549406553E-4	0.0
12	0.0	0.0	0.0	3.82272982410983E-4	0.0
13	0.0	0.0	0.0	4.4598514614614676E-4	0.0
14	0.0	0.0	0.0	5.096973098813106E-4	0.0
15	0.0	0.0	0.0	7.64545964821966E-4	0.0
16	0.0	0.0	0.0	9.556824560274573E-4	0.0
17	0.0	6.371216373516383E-5	0.0	0.0012105311109681127	0.0
18	0.0	1.2742432747032766E-4	0.0	0.001529091929643932	0.0
19	0.0	1.2742432747032766E-4	0.0	0.0019113649120549147	0.0
20	0.0	1.2742432747032766E-4	0.0	0.002229925730730734	0.0
21	0.0	1.2742432747032766E-4	0.0	0.0026121987131417167	0.0
22	0.0	1.2742432747032766E-4	0.0	0.0031856081867581913	0.0
23	0.0	3.1856081867581915E-4	0.0	0.004842124443872451	0.0
24	0.0	3.82272982410983E-4	0.0	0.007772883975689987	0.0
25	0.0	3.82272982410983E-4	0.0	0.010448794852566867	0.0
26	0.0	3.82272982410983E-4	0.0	0.01618288958873161	0.0
27	0.0	3.82272982410983E-4	0.0	0.054665036484770564	0.0
28	0.0	3.82272982410983E-4	0.0	0.18259906126497952	0.0
29	0.0	3.82272982410983E-4	0.0	0.35360250873015925	0.0
30	0.0	3.82272982410983E-4	0.0	0.5878721347843566	0.0
31	0.0	3.82272982410983E-4	0.0	1.2801047937669117	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1640	0.0	21.432926	1
CTTATAC	2100	0.0	17.442856	37
GTCCTAA	240	0.0	16.1875	1
GTATTAA	175	1.3096724E-10	15.857143	1
AGACCGT	140	3.475361E-8	15.857143	6
TCTTATA	3675	0.0	15.756461	37
CTATCGC	95	7.062306E-5	15.578948	30
ACCGTCG	215	0.0	15.488372	8
ATACACA	625	0.0	15.392	37
GACGGAC	370	0.0	15.000001	7
CGTCGTA	185	3.0559022E-10	15.000001	10
CAAGACG	370	0.0	15.000001	4
TACCGTC	210	9.094947E-12	14.9761915	7
AAGACGG	375	0.0	14.799999	5
GTATAAA	180	3.3323886E-9	14.388888	1
GCGGTAT	195	6.730261E-10	14.23077	24
CTTAGGG	130	4.4461667E-6	14.230769	3
CGCATCG	235	3.6379788E-12	14.170214	13
GATTTAT	185	4.9003575E-9	14.000001	1
GTATCAA	2515	0.0	13.902584	2
>>END_MODULE