FastQCFastQC Report
Fri 10 Feb 2017
ERR1632140.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632140.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478702
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.10152453927495603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT250.00549317929.66
CCGTTAT250.00549317929.69
CTTAGCC509.076624E-625.9000023
CGCATCG659.367977E-825.61538513
GGTATCA3100.023.8709681
TCGTTTA551.899653E-523.54545430
GCCTTAA502.6989114E-422.21
TAACGCC753.7313293E-722.24
TACCGTC502.6989114E-422.27
CGTACAC603.7204427E-521.5833323
ATAACGC909.449286E-820.5555553
GGTTAAC450.003822595820.5555551
ATGCACG656.89138E-519.9230776
GCGTACA656.89138E-519.9230772
AACGCCG851.2422042E-619.5882345
TTAGCCT609.226088E-418.54
GAATAAC1205.151378E-918.51
GCCATAT500.007029032318.51
TTAACCT609.226088E-418.54
CCGGTCG1002.866218E-718.520
Produced by FastQC (version 0.11.5)