##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632140.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	478702
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.578988598334664	31.0	31.0	34.0	30.0	34.0
2	31.76584388617553	31.0	31.0	34.0	30.0	34.0
3	31.86379208777068	31.0	31.0	34.0	30.0	34.0
4	35.6287941140835	37.0	35.0	37.0	33.0	37.0
5	34.940204970942254	37.0	35.0	37.0	32.0	37.0
6	34.87078182251171	36.0	35.0	37.0	32.0	37.0
7	34.85161123203997	36.0	35.0	37.0	32.0	37.0
8	34.79745436618188	35.0	35.0	37.0	32.0	37.0
9	36.448705039878675	38.0	35.0	39.0	32.0	39.0
10	36.19735033486386	37.0	35.0	39.0	32.0	39.0
11	36.33614858513229	38.0	35.0	39.0	32.0	39.0
12	36.244141449168794	38.0	35.0	39.0	32.0	39.0
13	36.35294191375846	38.0	35.0	39.0	32.0	39.0
14	37.382950144348676	39.0	36.0	40.0	32.0	41.0
15	37.3247907884237	39.0	36.0	40.0	32.0	41.0
16	37.30908581957042	39.0	36.0	40.0	32.0	41.0
17	37.253848949868605	39.0	36.0	40.0	32.0	41.0
18	37.243717385764	39.0	36.0	40.0	32.0	41.0
19	37.265190034718884	39.0	36.0	40.0	32.0	41.0
20	37.23455719842407	39.0	36.0	40.0	32.0	41.0
21	37.17994911239143	39.0	36.0	40.0	32.0	41.0
22	37.13157454951097	39.0	36.0	40.0	32.0	41.0
23	37.05581133983146	39.0	36.0	40.0	31.0	41.0
24	36.96381255979712	39.0	36.0	40.0	31.0	41.0
25	36.8657578201052	38.0	36.0	40.0	31.0	41.0
26	36.718497102581566	38.0	36.0	40.0	31.0	41.0
27	36.593855885289805	38.0	36.0	40.0	30.0	41.0
28	36.48242956996211	38.0	35.0	40.0	30.0	41.0
29	36.374416651695626	38.0	35.0	40.0	30.0	41.0
30	36.261511336906885	38.0	35.0	40.0	30.0	41.0
31	36.176895020284014	38.0	35.0	40.0	30.0	41.0
32	36.03410890282472	38.0	35.0	40.0	29.0	41.0
33	35.91299597662011	38.0	34.0	40.0	29.0	41.0
34	35.816428592318395	38.0	34.0	40.0	29.0	41.0
35	35.76815430058784	38.0	34.0	40.0	29.0	41.0
36	35.7096648854611	38.0	34.0	40.0	28.0	41.0
37	35.63350268016428	38.0	34.0	40.0	28.0	41.0
38	35.56380169708921	38.0	34.0	40.0	28.0	41.0
39	35.5002611227862	38.0	34.0	40.0	28.0	41.0
40	35.39916900284519	38.0	34.0	40.0	27.0	41.0
41	35.32248455197597	38.0	34.0	40.0	27.0	41.0
42	35.24913829480553	38.0	33.0	40.0	27.0	41.0
43	34.22444443516008	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	3.0
15	4.0
16	20.0
17	34.0
18	57.0
19	97.0
20	166.0
21	333.0
22	545.0
23	891.0
24	1428.0
25	2110.0
26	3201.0
27	4393.0
28	6232.0
29	8475.0
30	11509.0
31	15130.0
32	20485.0
33	27009.0
34	36778.0
35	50570.0
36	68846.0
37	87265.0
38	93077.0
39	40041.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.97433476358987	18.095391287272665	11.857272374044813	27.073001575092647
2	20.1540833336815	20.273364222418124	32.31133356451404	27.26121887938634
3	20.367577323679452	21.360470605930203	29.171802081461955	29.100149988928393
4	15.615769309507794	15.27589189098855	33.31550735112867	35.792831448374976
5	16.436530451094836	34.691937781751484	32.806213468922216	16.065318298231468
6	36.34641175512114	34.079448174438376	14.80231960593438	14.771820464506103
7	31.586038913562092	29.092002957998925	19.871861826355435	19.45009630208355
8	29.379446921049002	31.664793545880315	18.78308425701167	20.17267527605901
9	28.095976202313754	13.175837995245477	18.05486503085427	40.673320771586496
10	17.812960881717647	25.546582216075976	31.366486874924277	25.27397002728211
11	39.178236146913946	20.064048196999384	19.917401640268896	20.840314015817775
12	23.898166291346183	23.558915567513818	27.570597156477305	24.97232098466269
13	32.54780635969768	17.691799908920373	23.076569556843296	26.68382417453865
14	24.339986045598305	18.706627505212012	23.29737498485488	33.6560114643348
15	27.191446870913428	25.954560457236443	20.278586678142144	26.575405993707985
16	27.27709514478736	24.201068723339365	21.78578740009442	26.73604873177885
17	26.05316042130595	24.665031689861333	23.1239894548174	26.15781843401532
18	26.521510250636094	23.03959457031723	24.270005138896433	26.168890040150238
19	26.908598668900485	23.46950712551859	23.570404970106665	26.05148923547426
20	27.159903238340345	23.04627931364398	23.540114726907348	26.25370272110833
21	28.12960881717645	23.1074864947295	23.042519145522682	25.72038554257137
22	27.688624655840165	23.16973816695982	22.90840648252984	26.233230694670173
23	26.77636608996829	22.816491261787082	23.416238077133585	26.990904571111045
24	26.362747596625873	23.47243170072404	23.35753767479559	26.80728302785449
25	26.897109266307638	22.84176794749134	23.437545696487586	26.823577089713435
26	26.774486005907644	23.365684705725066	23.41059782495164	26.44923146341565
27	26.821697005652783	23.168484777586055	23.38970800205556	26.6201102147056
28	26.31511880042281	23.071138202890314	23.67443628812915	26.939306708557726
29	26.01722992592469	23.64372824847191	23.760502358461004	26.5785394671424
30	25.705971564773073	23.983605666991156	23.730629911719607	26.57979285651616
31	26.88729104954648	23.491650337788435	23.030194150013998	26.590864462651087
32	25.90170920530936	23.274187281440227	23.595263859353	27.228839653897413
33	25.726652489440195	23.259773303641932	23.896286207285534	27.11728799963234
34	26.508558560440527	22.87477386766715	23.963551437010917	26.65311613488141
35	26.253076026421446	22.95958654862524	24.581054601819087	26.206282823134224
36	26.011798571971706	23.294241511420466	24.134430188300865	26.559529728306963
37	26.182886221490616	22.301766025627636	24.7078558267983	26.80749192608345
38	25.308856031518566	22.340412197985383	25.4206165840126	26.930115186483448
39	25.597344485713453	21.939536496609584	25.617189817464727	26.845929200212243
40	25.285041633417034	22.02372248288079	26.051280337245302	26.63995554645688
41	24.57478765495026	21.884178465934966	27.005736345367264	26.53529753374751
42	24.781805799850428	21.75737724095575	27.111229950992477	26.349587008201343
43	23.469924921976514	21.253096916244342	27.538426829217343	27.7385513325618
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	11.0
2	14.0
3	26.0
4	38.0
5	38.0
6	59.5
7	81.0
8	72.0
9	63.0
10	102.0
11	141.0
12	141.0
13	232.5
14	324.0
15	606.5
16	889.0
17	899.5
18	910.0
19	910.0
20	1102.5
21	1295.0
22	1420.5
23	1546.0
24	1966.5
25	2387.0
26	2387.0
27	2916.0
28	3445.0
29	4325.5
30	5206.0
31	6371.5
32	7537.0
33	7537.0
34	9148.5
35	10760.0
36	12824.5
37	14889.0
38	16884.5
39	18880.0
40	18880.0
41	20784.5
42	22689.0
43	24674.0
44	26659.0
45	28861.0
46	31063.0
47	31063.0
48	33191.5
49	35320.0
50	36208.5
51	37097.0
52	38232.5
53	39368.0
54	39368.0
55	39044.5
56	38721.0
57	37762.0
58	36803.0
59	35737.0
60	34671.0
61	34671.0
62	32500.5
63	30330.0
64	27347.0
65	24364.0
66	21667.5
67	18971.0
68	18971.0
69	16030.5
70	13090.0
71	11171.5
72	9253.0
73	7124.5
74	4996.0
75	4996.0
76	3847.0
77	2698.0
78	2168.0
79	1638.0
80	1377.5
81	1117.0
82	1117.0
83	896.5
84	676.0
85	534.0
86	392.0
87	302.0
88	212.0
89	212.0
90	167.0
91	122.0
92	74.0
93	26.0
94	16.5
95	7.0
96	7.0
97	6.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	478702.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.3374815969718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.2996649005279	57.91239913760575
2	6.7100925317328	8.90261279675605
3	2.1250340894898687	4.229082294134157
4	1.0722457962257153	2.8452034309821506
5	0.6166977213822286	2.0455086871544017
6	0.41643107926955836	1.6574993434470862
7	0.2988842631904084	1.3879060516312738
8	0.2188096011862136	1.1612222313544946
9	0.16498615867482352	0.9850289638361556
>10	0.9730242622517997	11.719258145146693
>50	0.07005081946661618	3.216683384859044
>100	0.0340787766021592	3.9375955330927725
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	486	0.10152453927495603	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	2.0889822896081486E-4	0.0
18	0.0	0.0	0.0	4.177964579216297E-4	0.0
19	0.0	0.0	0.0	6.266946868824446E-4	0.0
20	0.0	0.0	0.0	6.266946868824446E-4	0.0
21	0.0	0.0	0.0	8.355929158432594E-4	0.0
22	0.0	0.0	0.0	0.0010444911448040744	0.0
23	0.0	0.0	0.0	0.0012533893737648892	0.0
24	0.0	0.0	0.0	0.0027156769764905934	0.0
25	0.0	0.0	0.0	0.004386862808177112	0.0
26	0.0	0.0	0.0	0.00731143801362852	0.0
27	0.0	0.0	0.0	0.018591942377512525	0.0
28	0.0	0.0	0.0	0.05995379171175387	0.0
29	0.0	2.0889822896081486E-4	0.0	0.13724613642725536	0.0
30	0.0	2.0889822896081486E-4	0.0	0.22790796779624903	0.0
31	0.0	2.0889822896081486E-4	0.0	0.48819516108142436	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	25	0.005493179	29.6	6
CCGTTAT	25	0.005493179	29.6	9
CTTAGCC	50	9.076624E-6	25.900002	3
CGCATCG	65	9.367977E-8	25.615385	13
GGTATCA	310	0.0	23.870968	1
TCGTTTA	55	1.899653E-5	23.545454	30
GCCTTAA	50	2.6989114E-4	22.2	1
TAACGCC	75	3.7313293E-7	22.2	4
TACCGTC	50	2.6989114E-4	22.2	7
CGTACAC	60	3.7204427E-5	21.583332	3
ATAACGC	90	9.449286E-8	20.555555	3
GGTTAAC	45	0.0038225958	20.555555	1
ATGCACG	65	6.89138E-5	19.923077	6
GCGTACA	65	6.89138E-5	19.923077	2
AACGCCG	85	1.2422042E-6	19.588234	5
TTAGCCT	60	9.226088E-4	18.5	4
GAATAAC	120	5.151378E-9	18.5	1
GCCATAT	50	0.0070290323	18.5	1
TTAACCT	60	9.226088E-4	18.5	4
CCGGTCG	100	2.866218E-7	18.5	20
>>END_MODULE