FastQCFastQC Report
Fri 10 Feb 2017
ERR1632139.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632139.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059552
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20510.1935723777596569No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17390.1641259702213766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13260.1251472320376914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.020.2526321
TATACGG555.1427295E-420.1818182
GTGTATC656.90148E-519.9230771
CTTATAC12250.017.97142837
TCGCCAT3000.017.88333313
CTACTAG650.001579823817.07692335
TCTAGCG1452.9776857E-916.58620628
AAACCGT803.382254E-416.18756
TAGTGCG803.382254E-416.18759
GTATACG700.00259231415.8571431
ACGGTTT700.00259231415.8571437
TCTATCC1059.34164E-615.8571423
GTTTAAG957.058841E-515.5789481
TAGTACC957.058841E-515.5789484
TAGAGGA1201.9350555E-615.4166664
TCTTATA20650.015.40920237
ATACCGC1954.1836756E-1115.17948727
TTGCGCG2109.094947E-1214.9761918
ATTTGCG2109.094947E-1214.9761916
CTTAAAC1252.9580315E-614.8000013
Produced by FastQC (version 0.11.5)