FastQCFastQC Report
Fri 10 Feb 2017
ERR1632138.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632138.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812660
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18520.10217029117429635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9350.021.9625681
AACGTCG450.003826646620.55555719
GTCGTTA555.144669E-420.18181812
CTTATAC15900.019.6635237
CCCGTAT500.007036409318.51
TCTAGAC1900.017.5263163
TAACGTC650.001580409817.07692318
GCGAAAG3100.016.70967918
GCGTTAT1901.8189894E-1216.5526311
AGACGGA3300.016.2575766
CGCAAGA3300.016.2575762
TCTTATA27700.016.2292437
CGAACGA1504.6748028E-916.03333316
CGGACCA3350.016.0149259
CAAGACG3700.016.04
CTAGCAC2900.015.9482763
CGTTATT2003.6379788E-1215.7252
CTAGACT1659.749783E-1015.696974
GAGCGAA3300.015.6969716
ACGGACC3550.015.6338028
Produced by FastQC (version 0.11.5)