##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632138.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1812660
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76102578530999	34.0	31.0	34.0	31.0	34.0
2	32.93311321483345	34.0	31.0	34.0	31.0	34.0
3	33.01888054020059	34.0	33.0	34.0	31.0	34.0
4	36.41253406595832	37.0	37.0	37.0	35.0	37.0
5	36.28057164608917	37.0	37.0	37.0	35.0	37.0
6	36.30415687442763	37.0	37.0	37.0	35.0	37.0
7	36.28102457162402	37.0	37.0	37.0	35.0	37.0
8	36.26561186322863	37.0	37.0	37.0	35.0	37.0
9	38.06025068131917	39.0	38.0	39.0	35.0	39.0
10	38.02877925259012	39.0	38.0	39.0	35.0	39.0
11	38.096092482870475	39.0	38.0	39.0	37.0	39.0
12	38.06637703706156	39.0	38.0	39.0	35.0	39.0
13	38.0796646916686	39.0	38.0	39.0	35.0	39.0
14	39.47945836505467	40.0	39.0	41.0	37.0	41.0
15	39.49551487868657	40.0	39.0	41.0	37.0	41.0
16	39.48492657199916	40.0	39.0	41.0	37.0	41.0
17	39.471312325532644	40.0	39.0	41.0	37.0	41.0
18	39.45721039797866	40.0	39.0	41.0	37.0	41.0
19	39.49145730583783	41.0	39.0	41.0	37.0	41.0
20	39.46646365010537	40.0	39.0	41.0	37.0	41.0
21	39.43569726258647	40.0	39.0	41.0	37.0	41.0
22	39.398726181412954	40.0	39.0	41.0	37.0	41.0
23	39.35001655026315	40.0	39.0	41.0	36.0	41.0
24	39.30444926240994	40.0	39.0	41.0	36.0	41.0
25	39.25137201681507	40.0	39.0	41.0	36.0	41.0
26	39.16872055432348	40.0	39.0	41.0	36.0	41.0
27	39.091999602793685	40.0	39.0	41.0	36.0	41.0
28	39.02357805655777	40.0	39.0	41.0	36.0	41.0
29	38.97081361093641	40.0	39.0	41.0	35.0	41.0
30	38.89587070934428	40.0	38.0	41.0	35.0	41.0
31	38.83299570796509	40.0	38.0	41.0	35.0	41.0
32	38.749197863912705	40.0	38.0	41.0	35.0	41.0
33	38.676147760749394	40.0	38.0	41.0	35.0	41.0
34	38.60704489534717	40.0	38.0	41.0	35.0	41.0
35	38.58326106385091	40.0	38.0	41.0	35.0	41.0
36	38.5440794191961	40.0	38.0	41.0	35.0	41.0
37	38.494147826950446	40.0	38.0	41.0	35.0	41.0
38	38.448987675570706	40.0	38.0	41.0	34.0	41.0
39	38.399969106175455	40.0	38.0	41.0	34.0	41.0
40	38.33815332163781	40.0	38.0	41.0	34.0	41.0
41	38.29463661138879	40.0	38.0	41.0	34.0	41.0
42	38.25037238092086	40.0	38.0	41.0	34.0	41.0
43	37.36654529807024	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	0.0
13	2.0
14	2.0
15	2.0
16	5.0
17	19.0
18	24.0
19	71.0
20	130.0
21	243.0
22	418.0
23	678.0
24	1135.0
25	1760.0
26	2691.0
27	3907.0
28	5834.0
29	8089.0
30	11239.0
31	15425.0
32	21288.0
33	29760.0
34	43520.0
35	64580.0
36	104535.0
37	191112.0
38	462401.0
39	843783.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.675570708240926	17.502013615349817	12.496221023247603	27.32619465316165
2	19.228978407423345	19.816898922026194	34.01205962508137	26.94206304546909
3	20.274844703364117	20.77576600134609	29.382178676640958	29.567210618648836
4	15.724349850495955	15.238820297242725	32.55111272935906	36.48571712290226
5	16.555890238654793	34.237088036366444	32.90710888969801	16.299912835280747
6	36.25428927653283	32.748116028378185	15.322564628777597	15.675030066311388
7	30.89459689075723	28.88765681374334	20.09516401310781	20.122582282391622
8	27.687707567883663	32.390189004005165	19.693268456301787	20.228834971809384
9	27.108613860293712	14.286297485463354	18.83949554797921	39.76559310626372
10	17.915439188816435	25.742279302240906	31.79813092361502	24.54415058532764
11	37.15015502079817	20.589575540917767	20.6382884821202	21.62198095616387
12	23.006134630873966	23.971125307559056	27.698520406474465	25.324219655092516
13	31.39982125715799	18.407202674522523	23.637582337559166	26.55539373076032
14	23.963401851422773	19.656802709829755	23.462094380633985	32.917701058113494
15	26.77104365959419	25.960191100371826	21.209824236205353	26.05894100382863
16	26.685699469288227	24.788101464146614	22.478622576765638	26.047576489799525
17	25.592995928635265	24.830801143071508	23.89041519093487	25.685787737358357
18	25.033266028929862	23.771584301523728	25.19308640340715	26.002063266139263
19	26.265598623018104	24.03534032857789	24.785839594849556	24.913221453554446
20	26.492006222898944	23.494422561318725	24.553694570410336	25.459876645371992
21	26.71113170699414	24.019507243498506	23.528571270949875	25.740789778557478
22	26.784394205201195	23.705052243664007	23.43451060871868	26.076042942416116
23	25.833802257455897	23.917888627762515	23.94442421634504	26.303884898436554
24	26.466904990456015	23.972063155804175	23.873699425154193	25.687332428585613
25	26.37289949576865	23.213840433396225	24.059614047863363	26.353646022971766
26	26.142299162556686	23.841150574294133	24.051173413657274	25.965376849491907
27	26.329096465967144	23.38590800260391	23.89670429093156	26.38829124049739
28	25.632164884755	23.862996921651053	24.33909282490925	26.165745368684696
29	25.391799896285015	24.213752165326095	24.45593768274249	25.938510255646396
30	25.22767645338894	24.878796906204144	24.216234704798474	25.677291935608444
31	26.12083898800658	23.95352686107709	24.004446504032746	25.92118764688359
32	24.899650237772114	23.884512263744995	24.554135910760984	26.661701587721907
33	25.16302009201946	23.523275186742136	24.868977083402292	26.444727637836106
34	25.868833647788332	23.164796486930808	24.818333278165788	26.14803658711507
35	25.65533525316386	23.138260898348285	25.125616497302307	26.080787351185553
36	24.95950702282833	23.31981728509483	25.405315944523515	26.31535974755332
37	25.564198470755684	22.0318206392815	25.628027318967707	26.775953570995114
38	24.50007171780698	22.218838612867277	27.014718700694008	26.266370968631737
39	24.319673849480875	21.734026237683846	27.435205719770945	26.511094193064338
40	23.843246940959695	21.67422462017146	28.296702084229807	26.18582635463904
41	23.089603124689685	21.796696567475422	29.063861948738317	26.049838359096576
42	23.42397360784703	21.483786258868182	28.717630443657388	26.3746096896274
43	22.24520869881831	21.42872905012523	29.32298390211071	27.003078348945746
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	57.0
1	59.5
2	62.0
3	146.0
4	230.0
5	230.0
6	330.0
7	430.0
8	426.5
9	423.0
10	624.0
11	825.0
12	825.0
13	1424.5
14	2024.0
15	3305.5
16	4587.0
17	4675.0
18	4763.0
19	4763.0
20	5612.5
21	6462.0
22	7337.5
23	8213.0
24	10038.5
25	11864.0
26	11864.0
27	14384.5
28	16905.0
29	22645.5
30	28386.0
31	32663.5
32	36941.0
33	36941.0
34	42960.0
35	48979.0
36	55246.5
37	61514.0
38	71316.0
39	81118.0
40	81118.0
41	89383.0
42	97648.0
43	98880.0
44	100112.0
45	107789.5
46	115467.0
47	115467.0
48	122062.5
49	128658.0
50	137288.0
51	145918.0
52	155187.5
53	164457.0
54	164457.0
55	152581.5
56	140706.0
57	135548.5
58	130391.0
59	120891.5
60	111392.0
61	111392.0
62	104863.5
63	98335.0
64	88002.5
65	77670.0
66	68163.0
67	58656.0
68	58656.0
69	50530.0
70	42404.0
71	37238.0
72	32072.0
73	26329.0
74	20586.0
75	20586.0
76	16832.0
77	13078.0
78	10956.5
79	8835.0
80	7290.0
81	5745.0
82	5745.0
83	4398.0
84	3051.0
85	2492.5
86	1934.0
87	1504.5
88	1075.0
89	1075.0
90	812.0
91	549.0
92	318.5
93	88.0
94	59.0
95	30.0
96	30.0
97	22.5
98	15.0
99	10.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1812660.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.71388492727719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.55128921856577	53.155788988507666
2	7.461281693640373	9.06006796315363
3	1.9311311801414508	3.5173942875175515
4	0.858640696372448	2.0852564973373586
5	0.45618257695036313	1.3848308241396559
6	0.3091476744456113	1.126173379909572
7	0.20498544464734575	0.8711823888659989
8	0.1462672268608176	0.7104361264207703
9	0.11385507009078562	0.6221325261491133
>10	0.7684455659304189	9.275823551038856
>50	0.09967038528275361	4.277172590689078
>100	0.0927625719991814	10.991557025630003
>500	0.005706625565471429	2.3467663362104796
>1k	6.340695072746032E-4	0.5754175144303113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1852	0.10217029117429635	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.5167543830613574E-5	0.0	0.0	0.0
3	0.0	5.5167543830613574E-5	0.0	5.5167543830613574E-5	0.0
4	0.0	5.5167543830613574E-5	0.0	1.1033508766122715E-4	5.5167543830613574E-5
5	0.0	5.5167543830613574E-5	0.0	1.1033508766122715E-4	5.5167543830613574E-5
6	0.0	5.5167543830613574E-5	0.0	1.1033508766122715E-4	5.5167543830613574E-5
7	0.0	5.5167543830613574E-5	0.0	1.1033508766122715E-4	5.5167543830613574E-5
8	0.0	5.5167543830613574E-5	0.0	1.1033508766122715E-4	5.5167543830613574E-5
9	0.0	1.1033508766122715E-4	0.0	2.206701753224543E-4	5.5167543830613574E-5
10	0.0	1.1033508766122715E-4	0.0	2.206701753224543E-4	1.6550263149184071E-4
11	0.0	1.1033508766122715E-4	0.0	2.7583771915306785E-4	1.6550263149184071E-4
12	0.0	1.1033508766122715E-4	0.0	3.86172806814295E-4	1.6550263149184071E-4
13	0.0	1.1033508766122715E-4	0.0	3.86172806814295E-4	1.6550263149184071E-4
14	0.0	1.1033508766122715E-4	0.0	3.86172806814295E-4	1.6550263149184071E-4
15	0.0	1.1033508766122715E-4	0.0	3.86172806814295E-4	2.206701753224543E-4
16	0.0	1.1033508766122715E-4	0.0	4.965078944755222E-4	2.206701753224543E-4
17	0.0	1.1033508766122715E-4	0.0	9.378482451204307E-4	2.206701753224543E-4
18	0.0	1.1033508766122715E-4	0.0	9.930157889510443E-4	2.7583771915306785E-4
19	0.0	1.1033508766122715E-4	0.0	0.001158518420442885	2.7583771915306785E-4
20	0.0	1.1033508766122715E-4	0.0	0.0012688535081041123	2.7583771915306785E-4
21	0.0	1.1033508766122715E-4	0.0	0.0013791885957653393	3.3100526298368143E-4
22	0.0	1.1033508766122715E-4	0.0	0.002041199121732702	3.3100526298368143E-4
23	0.0	1.1033508766122715E-4	0.0	0.002868712279191906	3.3100526298368143E-4
24	0.0	1.1033508766122715E-4	0.0	0.0046892412256021535	3.3100526298368143E-4
25	0.0	1.1033508766122715E-4	0.0	0.005958094733706266	3.3100526298368143E-4
26	0.0	1.1033508766122715E-4	0.0	0.009157812275881853	3.3100526298368143E-4
27	0.0	1.1033508766122715E-4	0.0	0.027418269283814946	3.3100526298368143E-4
28	0.0	1.1033508766122715E-4	0.0	0.10189445345514327	3.86172806814295E-4
29	0.0	1.6550263149184071E-4	0.0	0.20588527357584985	3.86172806814295E-4
30	0.0	1.6550263149184071E-4	0.0	0.34733485595754304	3.86172806814295E-4
31	0.0	1.6550263149184071E-4	0.0	0.8389880065759713	3.86172806814295E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	935	0.0	21.962568	1
AACGTCG	45	0.0038266466	20.555557	19
GTCGTTA	55	5.144669E-4	20.181818	12
CTTATAC	1590	0.0	19.66352	37
CCCGTAT	50	0.0070364093	18.5	1
TCTAGAC	190	0.0	17.526316	3
TAACGTC	65	0.0015804098	17.076923	18
GCGAAAG	310	0.0	16.709679	18
GCGTTAT	190	1.8189894E-12	16.552631	1
AGACGGA	330	0.0	16.257576	6
CGCAAGA	330	0.0	16.257576	2
TCTTATA	2770	0.0	16.22924	37
CGAACGA	150	4.6748028E-9	16.033333	16
CGGACCA	335	0.0	16.014925	9
CAAGACG	370	0.0	16.0	4
CTAGCAC	290	0.0	15.948276	3
CGTTATT	200	3.6379788E-12	15.725	2
CTAGACT	165	9.749783E-10	15.69697	4
GAGCGAA	330	0.0	15.69697	16
ACGGACC	355	0.0	15.633802	8
>>END_MODULE