FastQCFastQC Report
Fri 10 Feb 2017
ERR1632137.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632137.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956009
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52600.2689149180806428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45030.2302136646610522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37000.18916068382098447No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19640.10040853595254418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.024.2159461
TAGCGAA801.6176158E-518.510
TACCGTC1351.1532393E-917.8148147
ATACCGT1251.6593367E-716.2799996
CTTATAC24400.016.1495937
TATACAC9300.015.91397937
ACCGTCG1403.4768163E-815.8571438
TATACTG1751.3278623E-1015.8571425
TCGCGTA1302.5914778E-715.6538469
TCTTATA40350.015.13011137
GTATCAA24500.014.875512
CGCGTAA1252.9611329E-614.79999910
ATACACA6200.014.3225837
CCGTCGT1458.918396E-714.0344839
CGTCGTA1409.566222E-613.21428610
CCGAATA1551.8892479E-613.12903235
CCTAAGG1703.7347127E-713.0588243
GTCGTAC850.00940781513.0588241
ATTCGTA2305.7661964E-1012.86956424
TAGACAG2602.3646862E-1112.8076935
Produced by FastQC (version 0.11.5)