FastQCFastQC Report
Fri 10 Feb 2017
ERR1632136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241770
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.23410679571493567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5510.22790255201224305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3560.1472473838772387No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG2970.1228440253133143No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT2880.11912147909169872No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA2810.1162261653637755No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC2530.10464491045208257No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC2480.10257682921785169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAAC200.001839062937.023
GCAATAT303.591148E-430.83333436
TCCGAAA250.00548786829.59999821
ATATTGA250.00548786829.5999986
TTTTAGC451.3189942E-424.66666631
TTTAGCA400.00192680323.12532
TTAGCAA400.00192680323.12533
CAATATT400.00192680323.12537
GACCGCG450.003817205820.5555557
TAGCAAT450.003817205820.55555534
GTTGCAA450.003817205820.5555558
GGTATCA2600.019.9230751
GTCGCCA1052.237357E-819.3809512
TCTTATA3750.019.24000237
TGATATT609.208081E-418.526
ATTTTAG609.208081E-418.530
GAACACT500.007019215718.4999986
AATACTG500.007019215718.4999985
CTTATAC2050.018.0487837
TCGCCAT1258.480129E-917.7613