##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632136.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241770
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73235306282831	34.0	31.0	34.0	31.0	34.0
2	32.88800099267899	34.0	31.0	34.0	31.0	34.0
3	32.87734210199777	34.0	31.0	34.0	31.0	34.0
4	36.347102618190846	37.0	37.0	37.0	35.0	37.0
5	36.254460851222234	37.0	37.0	37.0	35.0	37.0
6	36.29394052198371	37.0	37.0	37.0	35.0	37.0
7	36.28661537825206	37.0	37.0	37.0	35.0	37.0
8	36.26760143938454	37.0	37.0	37.0	35.0	37.0
9	38.0308061380651	39.0	38.0	39.0	35.0	39.0
10	37.9873309343591	39.0	38.0	39.0	35.0	39.0
11	38.08206146337428	39.0	38.0	39.0	37.0	39.0
12	38.0572982586756	39.0	38.0	39.0	35.0	39.0
13	38.0748852214915	39.0	38.0	39.0	35.0	39.0
14	39.45623112875874	40.0	39.0	41.0	37.0	41.0
15	39.475327790875625	40.0	39.0	41.0	37.0	41.0
16	39.357658104810355	40.0	39.0	41.0	37.0	41.0
17	39.40905819580593	40.0	39.0	41.0	37.0	41.0
18	39.420184472846096	40.0	39.0	41.0	37.0	41.0
19	39.48346362245109	41.0	39.0	41.0	37.0	41.0
20	39.47158456384167	41.0	39.0	41.0	37.0	41.0
21	39.43500434297059	40.0	39.0	41.0	37.0	41.0
22	39.41503495057286	40.0	39.0	41.0	37.0	41.0
23	39.37008313686562	40.0	39.0	41.0	37.0	41.0
24	39.310009513173675	40.0	39.0	41.0	36.0	41.0
25	39.25454771063407	40.0	39.0	41.0	36.0	41.0
26	39.15601604831038	40.0	39.0	41.0	36.0	41.0
27	39.08315341026596	40.0	39.0	41.0	36.0	41.0
28	39.01268974645324	40.0	39.0	41.0	35.0	41.0
29	38.88042354303677	40.0	38.0	41.0	35.0	41.0
30	38.819671588700004	40.0	38.0	41.0	35.0	41.0
31	38.74100177854986	40.0	38.0	41.0	35.0	41.0
32	38.66399470571204	40.0	38.0	41.0	35.0	41.0
33	38.529920999296856	40.0	38.0	41.0	35.0	41.0
34	38.52086280349092	40.0	38.0	41.0	35.0	41.0
35	38.48965959382885	40.0	38.0	41.0	35.0	41.0
36	38.45297183273359	40.0	38.0	41.0	35.0	41.0
37	38.40843363527319	40.0	38.0	41.0	34.0	41.0
38	38.327654382264136	40.0	38.0	41.0	34.0	41.0
39	38.12848161475783	40.0	38.0	41.0	34.0	41.0
40	38.117727592339826	40.0	38.0	41.0	34.0	41.0
41	38.101244984903005	40.0	38.0	41.0	34.0	41.0
42	37.95040327584068	40.0	37.0	41.0	33.0	41.0
43	37.078860073623694	39.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	6.0
19	12.0
20	13.0
21	44.0
22	59.0
23	110.0
24	146.0
25	274.0
26	418.0
27	580.0
28	844.0
29	1241.0
30	1734.0
31	2293.0
32	3025.0
33	4160.0
34	6235.0
35	9084.0
36	14473.0
37	26488.0
38	59794.0
39	110734.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.889523100467386	17.81362451917111	13.050006204243703	26.2468461761178
2	19.74273069446168	20.312280266368862	33.580262232700505	26.36472680646896
3	21.135376597592753	21.243744054266454	29.43127766058651	28.189601687554287
4	16.238573851180874	15.658270256855689	31.586218306655084	36.51693758530835
5	16.61206932208297	34.3648922529677	32.276957438888196	16.74608098606113
6	36.0193572403524	32.024651528312035	15.448980435951523	16.50701079538404
7	29.80187781776068	29.070604293336643	20.057906274558466	21.06961161434421
8	27.255242585928773	32.334036480952975	19.688133349877983	20.72258758324027
9	26.286139719568187	14.503867311908012	19.169044960086033	40.04094800843777
10	18.53331678868346	25.961450965793937	31.466683211316543	24.038549034206063
11	36.850312280266365	20.73210075691773	20.461182115233488	21.956404847582416
12	22.56772966042106	24.682963146792407	27.25358812094139	25.49571907184514
13	31.343425569756377	18.66319229019316	23.85159449063159	26.14178764941887
14	23.86483021053067	20.4649046614551	23.231583736609174	32.438681391405055
15	26.896223683666292	26.532241386441658	21.17260205980891	25.398932870083136
16	26.40277950117881	25.079621127517886	22.815899408528768	25.701699962774537
17	25.240931463787895	25.64172560698184	23.801546924763205	25.315796004467057
18	24.415767051329777	24.081978740124914	25.484965049427146	26.017289159118167
19	25.93746122347686	24.697439715432022	25.155726516937587	24.209372544153535
20	26.04748314513794	23.406543409025108	25.456839144641602	25.089134301195347
21	26.181494809116103	24.331802953220002	24.042685196674526	25.44401704098937
22	26.22161558506018	23.855317036853208	23.885924639119825	26.037142738966786
23	25.050667990238658	24.35496546304339	24.437688712412623	26.15667783430533
24	26.317574554328495	24.508830706870167	24.339661661910082	24.83393307689126
25	25.882863878893165	23.54882739794019	24.59817181618894	25.970136906977707
26	26.012325764156014	24.194068743020225	24.49559498697109	25.29801050585267
27	25.952351408363324	23.565785664060883	24.289200479794847	26.19266244778095
28	24.837655623112877	24.046407742896143	25.233486371344664	25.882450262646316
29	25.007651900566653	24.570045911403398	24.895148281424497	25.52715390660545
30	24.435207014931546	25.364602721594903	24.94436861479919	25.25582164867436
31	25.772841957232078	24.008768664433138	24.671381891880714	25.547007486454063
32	24.462505687223395	24.193241510526533	24.956777102204576	26.3874757000455
33	24.8566819704678	23.693593084336353	25.25375356744013	26.19597137775572
34	25.268643752326593	23.847044711916286	24.914588245026266	25.96972329073086
35	25.24258592877528	23.603424742523888	25.280225007238283	25.873764321462545
36	24.569632295156556	23.89088803408198	25.673987674235843	25.865491996525623
37	25.171857550564585	22.306737808661122	26.036729122719944	26.484675518054352
38	23.93059519377921	22.592546635231834	27.48852214915002	25.988336021838936
39	23.899987591512595	22.048641270629112	28.07999338214005	25.971377755718244
40	23.51656533068619	21.927865326550027	28.60694048062208	25.948628862141703
41	22.58716962402283	22.412623567853746	29.203788724821113	25.796418083302314
42	23.04297472804732	22.15121809984696	28.646647640319312	26.159159531786408
43	21.784340488894404	22.441990321379823	29.12520163792034	26.64846755180543
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	11.5
2	11.0
3	43.0
4	75.0
5	75.0
6	104.5
7	134.0
8	135.5
9	137.0
10	224.5
11	312.0
12	312.0
13	527.0
14	742.0
15	1110.5
16	1479.0
17	1354.5
18	1230.0
19	1230.0
20	1335.0
21	1440.0
22	1455.5
23	1471.0
24	1568.5
25	1666.0
26	1666.0
27	1934.0
28	2202.0
29	3155.5
30	4109.0
31	4421.0
32	4733.0
33	4733.0
34	5262.5
35	5792.0
36	6405.0
37	7018.0
38	8808.5
39	10599.0
40	10599.0
41	11828.0
42	13057.0
43	13001.0
44	12945.0
45	14316.5
46	15688.0
47	15688.0
48	17221.5
49	18755.0
50	19796.5
51	20838.0
52	21704.5
53	22571.0
54	22571.0
55	20150.0
56	17729.0
57	17000.5
58	16272.0
59	15167.5
60	14063.0
61	14063.0
62	13215.0
63	12367.0
64	11082.0
65	9797.0
66	8756.5
67	7716.0
68	7716.0
69	6601.0
70	5486.0
71	4881.0
72	4276.0
73	3452.0
74	2628.0
75	2628.0
76	2165.5
77	1703.0
78	1417.5
79	1132.0
80	920.5
81	709.0
82	709.0
83	554.5
84	400.0
85	322.0
86	244.0
87	188.5
88	133.0
89	133.0
90	105.0
91	77.0
92	48.5
93	20.0
94	10.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	241770.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.71535320796568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.489093773583	61.27500946346901
2	5.48665799181178	7.430619676936848
3	1.3357961585216676	2.713617260644153
4	0.581475277908286	1.5749921530103836
5	0.3509535247782072	1.188247094496842
6	0.24957630612851195	1.0140088633098934
7	0.18705010066954772	0.8866314544096775
8	0.15383783877767437	0.833374687166424
9	0.1049423505149295	0.6395587498433333
>10	0.9130241274402456	13.054684679348243
>50	0.10916969597538123	5.013660296319057
>100	0.037203080751386904	3.9142893153634284
>500	0.0012197731393897345	0.4613063056827337
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	566	0.23410679571493567	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	551	0.22790255201224305	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	356	0.1472473838772387	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	297	0.1228440253133143	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	288	0.11912147909169872	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	281	0.1162261653637755	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	253	0.10464491045208257	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	248	0.10257682921785169	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	4.136162468461761E-4	0.0	0.0	0.0
10	0.0	4.136162468461761E-4	0.0	0.0	0.0
11	0.0	4.136162468461761E-4	0.0	0.0	0.0
12	0.0	4.136162468461761E-4	0.0	0.0	0.0
13	0.0	4.136162468461761E-4	0.0	0.0	0.0
14	0.0	4.136162468461761E-4	0.0	0.0	0.0
15	0.0	4.136162468461761E-4	0.0	0.0	0.0
16	0.0	4.136162468461761E-4	0.0	4.136162468461761E-4	0.0
17	0.0	4.136162468461761E-4	0.0	4.136162468461761E-4	0.0
18	0.0	4.136162468461761E-4	0.0	4.136162468461761E-4	0.0
19	0.0	4.136162468461761E-4	0.0	4.136162468461761E-4	0.0
20	0.0	4.136162468461761E-4	0.0	8.272324936923522E-4	0.0
21	0.0	4.136162468461761E-4	0.0	0.0016544649873847044	0.0
22	0.0	4.136162468461761E-4	0.0	0.002481697481077057	0.0
23	0.0	4.136162468461761E-4	0.0	0.004136162468461761	0.0
24	0.0	4.136162468461761E-4	0.0	0.009513173677462052	0.0
25	0.0	4.136162468461761E-4	0.0	0.011581254911692932	0.0
26	0.0	4.136162468461761E-4	0.0	0.016131033627000867	0.0
27	0.0	4.136162468461761E-4	0.0	0.04467055465938702	0.0
28	0.0	4.136162468461761E-4	0.0	0.12780742027546843	0.0
29	0.0	4.136162468461761E-4	0.0	0.2353476444554742	0.0
30	0.0	4.136162468461761E-4	0.0	0.38011333085163584	0.0
31	0.0	4.136162468461761E-4	0.0	0.7614675104438102	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAAC	20	0.0018390629	37.0	23
GCAATAT	30	3.591148E-4	30.833334	36
TCCGAAA	25	0.005487868	29.599998	21
ATATTGA	25	0.005487868	29.599998	6
TTTTAGC	45	1.3189942E-4	24.666666	31
TTTAGCA	40	0.001926803	23.125	32
TTAGCAA	40	0.001926803	23.125	33
CAATATT	40	0.001926803	23.125	37
GACCGCG	45	0.0038172058	20.555555	7
TAGCAAT	45	0.0038172058	20.555555	34
GTTGCAA	45	0.0038172058	20.555555	8
GGTATCA	260	0.0	19.923075	1
GTCGCCA	105	2.237357E-8	19.38095	12
TCTTATA	375	0.0	19.240002	37
TGATATT	60	9.208081E-4	18.5	26
ATTTTAG	60	9.208081E-4	18.5	30
GAACACT	50	0.0070192157	18.499998	6
AATACTG	50	0.0070192157	18.499998	5
CTTATAC	205	0.0	18.04878	37
TCGCCAT	125	8.480129E-9	17.76	13
>>END_MODULE