FastQCFastQC Report
Fri 10 Feb 2017
ERR1632135.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632135.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4878408
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27000.026.7907411
CTTATAC30100.019.29900237
CGTCTAA2250.018.0888881
TCTTATA54150.017.28716537
TATAGCG752.0689989E-417.2666665
GTATCAA42250.016.7704122
CGAATTA1005.8856585E-616.65000215
TAATACG3300.016.2575764
GTACTAT8100.015.7592591
ATAATAC4600.014.4782613
CTCGCTA2600.014.23076913
TATTAGA5350.014.1775692
ATACCGT4750.014.0210526
GTATAGA6100.013.9508191
CTATATC6900.013.6739124
CTCTTAT87150.013.62822737
TACCGTC4800.013.4895847
GTAATAC5800.013.3965513
ACCGTCG4500.012.7444458
TCGCTAA2951.8189894E-1212.54237314