##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632134.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	700330
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.312289920466068	31.0	31.0	34.0	30.0	34.0
2	31.494455470992246	31.0	31.0	34.0	30.0	34.0
3	31.51766167378236	31.0	31.0	34.0	30.0	34.0
4	35.38296231776448	37.0	35.0	37.0	33.0	37.0
5	34.61276969428698	35.0	35.0	37.0	30.0	37.0
6	34.55458283951851	35.0	35.0	37.0	30.0	37.0
7	34.54954378650065	35.0	35.0	37.0	30.0	37.0
8	34.48637642254366	35.0	35.0	37.0	30.0	37.0
9	36.09734268130738	37.0	35.0	39.0	32.0	39.0
10	35.7749132551797	37.0	35.0	39.0	30.0	39.0
11	35.97714220438936	37.0	35.0	39.0	31.0	39.0
12	35.88087187468765	37.0	35.0	39.0	30.0	39.0
13	35.97871717618837	37.0	35.0	39.0	31.0	39.0
14	36.95146430968258	38.0	36.0	40.0	32.0	41.0
15	36.90902003341282	38.0	36.0	40.0	31.0	41.0
16	36.78745305784416	38.0	36.0	40.0	31.0	41.0
17	36.78091613953422	38.0	36.0	40.0	31.0	41.0
18	36.753713249468106	38.0	36.0	40.0	31.0	41.0
19	36.81230419944883	38.0	36.0	40.0	31.0	41.0
20	36.76306598317936	38.0	36.0	40.0	31.0	41.0
21	36.715801122328045	38.0	36.0	40.0	31.0	41.0
22	36.67787328830694	38.0	36.0	40.0	31.0	41.0
23	36.59839218654063	38.0	35.0	40.0	31.0	41.0
24	36.48372338754587	38.0	35.0	40.0	30.0	41.0
25	36.36413976268331	38.0	35.0	40.0	30.0	41.0
26	36.163884168891805	38.0	35.0	40.0	30.0	41.0
27	36.05496551625662	38.0	35.0	40.0	29.0	41.0
28	35.90494195593506	38.0	34.0	40.0	29.0	41.0
29	35.71352219667871	38.0	34.0	40.0	28.0	41.0
30	35.60219753544757	38.0	34.0	40.0	28.0	41.0
31	35.48564819442263	38.0	34.0	40.0	27.0	41.0
32	35.34590978538689	38.0	34.0	40.0	27.0	41.0
33	35.179121271400625	38.0	33.0	40.0	27.0	41.0
34	35.11862264932246	38.0	33.0	40.0	27.0	41.0
35	35.04587551582825	38.0	33.0	40.0	26.0	41.0
36	35.00417231876401	38.0	33.0	40.0	26.0	40.0
37	34.97367098367912	38.0	33.0	40.0	26.0	40.0
38	34.88590664401068	37.0	33.0	40.0	26.0	40.0
39	34.69825082461126	37.0	33.0	40.0	26.0	40.0
40	34.63392686305028	37.0	33.0	39.0	26.0	40.0
41	34.60677252152556	37.0	33.0	39.0	26.0	40.0
42	34.486349292476405	37.0	33.0	39.0	25.0	40.0
43	33.46631730755501	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	3.0
13	7.0
14	8.0
15	17.0
16	24.0
17	59.0
18	133.0
19	217.0
20	379.0
21	694.0
22	1127.0
23	1865.0
24	2688.0
25	4121.0
26	5911.0
27	8352.0
28	11389.0
29	15613.0
30	20766.0
31	27644.0
32	36007.0
33	47627.0
34	63162.0
35	82445.0
36	103978.0
37	120271.0
38	109607.0
39	36213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03828195279368	17.499036168663345	11.846558051204433	27.61612382733854
2	21.54941241985921	19.36072994159896	31.002527379949452	28.08733025859238
3	21.943655134008253	20.172490111804436	27.9206945297217	29.963160224465607
4	16.940727942541372	14.821298530692673	31.195722016763526	37.04225151000243
5	17.80931846415261	33.31343795068039	31.69634315251382	17.18090043265318
6	38.279239786957575	31.67663815629774	14.269701426470377	15.7744206302743
7	32.46997843873602	27.708508845829822	18.96306027158625	20.8584524438479
8	29.876058429597474	30.482915197121358	18.267388231262405	21.373638142018763
9	28.038210557879857	12.945040195336485	17.45719874916111	41.55955049762255
10	18.91651078777148	24.515157140205332	30.646695129438978	25.921636942584207
11	39.60789913326575	19.077863293019004	19.054874130766926	22.259363442948324
12	24.64937957819885	22.706723972984165	26.15895363614296	26.484942812674024
13	33.37683663415818	17.0541030656976	21.747604700641126	27.821455599503093
14	24.97465480559165	18.248682763839906	22.136135821684064	34.64052660888438
15	28.46386703411249	24.42234375223109	19.380720517470333	27.733068696186088
16	28.193851470021276	22.973883740522325	20.865020775919923	27.967244013536472
17	27.029257635686033	23.42138706038582	22.356460525752144	27.192894778176
18	27.082375451572833	21.879256921736896	23.230477060814188	27.807890565876086
19	27.78761441034941	22.459840360972684	22.828380906144247	26.924164322533663
20	27.735210543600875	22.20381819999143	22.60834178173147	27.452629474676225
21	28.98376479659589	22.152699441691773	21.757742778404467	27.105792983307868
22	29.07086659146402	22.184398783430666	21.633515628346636	27.111218996758673
23	28.378478717176186	21.991775305927206	21.64650950266303	27.983236474233575
24	28.024217154769893	22.22495109448403	21.860123084831436	27.890708665914637
25	28.009652592349322	21.590964259706137	22.217954392929048	28.181428755015492
26	27.892564933674123	22.128710750646125	22.45484271700484	27.523881598674908
27	28.06933874030814	22.08458869390145	22.1034369511516	27.74263561463881
28	27.474333528479434	22.046463809918183	22.819385147002127	27.65981751460026
29	27.351391486870476	22.335184841431897	22.962174974654808	27.351248697042823
30	27.03753944568989	22.609769680007997	22.83651992632045	27.516170947981667
31	27.583853326289038	22.428997758199706	22.231662216383704	27.75548669912755
32	26.947296274613397	22.431139605614497	22.859223508917225	27.762340610854885
33	27.245441434752188	22.16540773635286	22.923764511016234	27.665386317878713
34	27.590278868533407	22.112718289949022	22.90006139962589	27.39694144189168
35	27.400511187583	21.948509988148444	23.674839004469323	26.97613981979924
36	26.937729356160666	22.12571216426542	23.093256036439964	27.84330244313395
37	26.395128011080494	21.387488755301074	24.06179943740808	28.155583796210358
38	26.284894264132625	21.384632958748018	24.731769308754444	27.598703468364917
39	25.779275484414487	21.104050947410506	25.451001670640984	27.665671897534022
40	25.559807519312322	20.757357245869805	26.469807090942844	27.21302814387503
41	24.811874402067595	20.754787028972054	26.889466394414065	27.543872174546287
42	24.597975240243887	21.04550711807291	26.942584210300858	27.413933431382347
43	23.944283409249927	20.007139491382635	27.59342024474176	28.455156854625674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	7.0
2	9.0
3	18.5
4	28.0
5	28.0
6	39.0
7	50.0
8	55.0
9	60.0
10	84.0
11	108.0
12	108.0
13	231.5
14	355.0
15	556.5
16	758.0
17	716.0
18	674.0
19	674.0
20	864.5
21	1055.0
22	1084.5
23	1114.0
24	1525.5
25	1937.0
26	1937.0
27	2484.0
28	3031.0
29	4050.0
30	5069.0
31	6485.0
32	7901.0
33	7901.0
34	10123.5
35	12346.0
36	14927.5
37	17509.0
38	20304.5
39	23100.0
40	23100.0
41	26091.5
42	29083.0
43	32111.0
44	35139.0
45	38320.0
46	41501.0
47	41501.0
48	44370.5
49	47240.0
50	50053.5
51	52867.0
52	55439.5
53	58012.0
54	58012.0
55	58340.5
56	58669.0
57	58091.5
58	57514.0
59	55037.0
60	52560.0
61	52560.0
62	50988.0
63	49416.0
64	44146.0
65	38876.0
66	34455.0
67	30034.0
68	30034.0
69	26147.0
70	22260.0
71	19674.0
72	17088.0
73	15583.0
74	14078.0
75	14078.0
76	12168.5
77	10259.0
78	7680.0
79	5101.0
80	3894.5
81	2688.0
82	2688.0
83	2058.5
84	1429.0
85	1114.0
86	799.0
87	589.5
88	380.0
89	380.0
90	282.5
91	185.0
92	109.5
93	34.0
94	20.0
95	6.0
96	6.0
97	4.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	700330.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.50263495065383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.77247608879917	65.98513538571305
2	6.0391634690580105	8.877888557469902
3	1.6550951477753075	3.6496156336658063
4	0.738620346917719	2.1716216690647356
5	0.43267077629900436	1.590122106206086
6	0.25874556802928134	1.14110886191735
7	0.17332906175540416	0.8918099926782764
8	0.1364148410350922	0.8021480209963082
9	0.10202753987559228	0.6749363716550042
>10	0.6140261466836812	8.426917746049515
>50	0.05071078193423171	2.542635756598148
>100	0.02652519364902563	3.155887585349966
>500	1.9503818847371973E-4	0.09017231263588182
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4278982765267802E-4	0.0	0.0	0.0	0.0
2	1.4278982765267802E-4	0.0	0.0	0.0	0.0
3	1.4278982765267802E-4	0.0	0.0	0.0	0.0
4	1.4278982765267802E-4	0.0	0.0	0.0	0.0
5	1.4278982765267802E-4	0.0	0.0	0.0	0.0
6	1.4278982765267802E-4	0.0	0.0	0.0	0.0
7	1.4278982765267802E-4	0.0	0.0	0.0	0.0
8	1.4278982765267802E-4	0.0	0.0	0.0	0.0
9	1.4278982765267802E-4	0.0	0.0	1.4278982765267802E-4	0.0
10	1.4278982765267802E-4	0.0	0.0	2.8557965530535604E-4	0.0
11	1.4278982765267802E-4	0.0	0.0	2.8557965530535604E-4	0.0
12	1.4278982765267802E-4	0.0	0.0	2.8557965530535604E-4	0.0
13	1.4278982765267802E-4	0.0	0.0	2.8557965530535604E-4	0.0
14	1.4278982765267802E-4	0.0	0.0	2.8557965530535604E-4	0.0
15	2.8557965530535604E-4	0.0	0.0	2.8557965530535604E-4	0.0
16	2.8557965530535604E-4	0.0	0.0	2.8557965530535604E-4	0.0
17	2.8557965530535604E-4	0.0	0.0	4.283694829580341E-4	0.0
18	2.8557965530535604E-4	0.0	0.0	4.283694829580341E-4	0.0
19	2.8557965530535604E-4	0.0	0.0	4.283694829580341E-4	0.0
20	2.8557965530535604E-4	0.0	0.0	4.283694829580341E-4	0.0
21	2.8557965530535604E-4	0.0	0.0	5.711593106107121E-4	0.0
22	2.8557965530535604E-4	0.0	0.0	0.0011423186212214242	0.0
23	2.8557965530535604E-4	1.4278982765267802E-4	0.0	0.0014278982765267801	0.0
24	2.8557965530535604E-4	1.4278982765267802E-4	0.0	0.0024274270700955264	0.0
25	2.8557965530535604E-4	1.4278982765267802E-4	0.0	0.0038553253466223065	0.0
26	2.8557965530535604E-4	1.4278982765267802E-4	0.0	0.006425542244370511	0.0
27	2.8557965530535604E-4	1.4278982765267802E-4	0.0	0.02370311139034455	0.0
28	2.8557965530535604E-4	1.4278982765267802E-4	0.0	0.07724929676009881	0.0
29	2.8557965530535604E-4	1.4278982765267802E-4	0.0	0.15949623748804134	0.0
30	2.8557965530535604E-4	1.4278982765267802E-4	0.0	0.27187183185069896	0.0
31	2.8557965530535604E-4	1.4278982765267802E-4	0.0	0.6366998415032913	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAATAT	35	8.86592E-4	26.428572	3
GTTTACA	45	1.3224909E-4	24.666666	1
GGTATCA	430	0.0	23.232557	1
GTGTCGT	45	0.0038243376	20.555555	10
GTATAGA	65	6.897209E-5	19.923077	1
AGTACGT	60	9.2319027E-4	18.5	5
TCTTATA	950	0.0	17.526316	37
CTTATAC	500	0.0	16.279999	37
GTATCAA	605	0.0	16.206612	2
TAGGCAG	115	1.2404234E-6	16.086956	5
TCCGGAT	70	0.0025911373	15.857143	18
CCCTATG	70	0.0025911373	15.857143	1
TAATACA	120	1.9327745E-6	15.416667	4
GTATTAG	110	1.45017875E-5	15.136364	1
GTACGTG	75	0.0041022766	14.8	6
ATTAGCA	90	8.268323E-4	14.388888	3
CCTATGG	90	8.268323E-4	14.388888	2
TGCATCA	245	0.0	14.346938	14
TTATTCA	105	1.6541732E-4	14.095238	4
GCTGCAT	290	0.0	14.034483	12
>>END_MODULE