FastQCFastQC Report
Fri 10 Feb 2017
ERR1632133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18309
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT3641.9880932874542574TruSeq Adapter, Index 12 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2671.4582991971161725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2601.420066633895898No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT2081.1360533071167185No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC1570.8575017750832924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1480.8083456223715113No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC1460.7974220328800044No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG1080.5898738325413732No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC1010.5516412693210989RNA PCR Primer, Index 12 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.40417281118575565No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG570.3113223005079469No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.16385384237260364No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.1583920476268502No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT210.11469768966082254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC200.001795016137.03
CGTCTTC250.00535791429.637
CCGTCTT855.638867E-1126.11764737
TCTCTTA508.470228E-625.90000237
GGTATCA1750.025.371431
GCCGTCT901.1641532E-1024.66666836
TGCCGTC1058.094503E-1021.14285935
ATGCCGT1101.4460966E-920.18181834
GTATCAA2350.018.8936182
CATCAGC500.00678008818.524
CATGGGC852.4941568E-517.41176418
TATGCCG1351.8366336E-816.44444533
GTCTCTT803.1608916E-416.187536
GTATGCC1454.4306944E-815.31034632
CGTATGC1454.4306944E-815.31034631
CAACGCA2900.015.3103466
TCAACGC2950.015.0508475
TATCAAC2950.015.0508473
ATCAACG2950.015.0508474
AACGCAG3150.014.0952387
Produced by FastQC (version 0.11.5)