Basic Statistics
Measure | Value |
---|---|
Filename | ERR1632132.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 319060 |
Sequences flagged as poor quality | 0 |
Sequence length | 43 |
%GC | 50 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT | 840 | 0.26327336551118913 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT | 691 | 0.2165736852002758 | No Hit |
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT | 599 | 0.18773898326333605 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT | 495 | 0.15514323324766502 | No Hit |
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC | 383 | 0.12004011784617313 | No Hit |
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA | 362 | 0.11345828370839342 | No Hit |
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC | 340 | 0.10656302889738609 | No Hit |
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT | 335 | 0.10499592553124804 | RNA PCR Primer, Index 17 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTTAGTA | 20 | 0.001839944 | 37.0 | 2 |
CAATCGC | 20 | 0.001839944 | 37.0 | 33 |
TTAGGTT | 25 | 0.0054904683 | 29.599998 | 4 |
GCACTTT | 25 | 0.0054904683 | 29.599998 | 6 |
AAAGGCC | 40 | 0.0019281473 | 23.125 | 17 |
ACATGTT | 50 | 2.6961783E-4 | 22.199999 | 29 |
GGTATCA | 665 | 0.0 | 21.977444 | 1 |
GCCGTCT | 60 | 3.715435E-5 | 21.583334 | 36 |
GTCACTA | 45 | 0.0038198426 | 20.555557 | 1 |
TTAACGG | 45 | 0.0038198426 | 20.555557 | 35 |
CCCCAGA | 45 | 0.0038198426 | 20.555557 | 1 |
ACTGCGG | 55 | 5.131909E-4 | 20.181818 | 8 |
CACATGT | 70 | 1.2156975E-4 | 18.5 | 28 |
GATCAGT | 60 | 9.216891E-4 | 18.5 | 1 |
ATACCGC | 70 | 1.2156975E-4 | 18.5 | 27 |
ATCAGTA | 50 | 0.007024021 | 18.499998 | 2 |
CGCATCT | 50 | 0.007024021 | 18.499998 | 15 |
TAACGGC | 50 | 0.007024021 | 18.499998 | 36 |
ACATCGT | 50 | 0.007024021 | 18.499998 | 8 |
TGCACAG | 50 | 0.007024021 | 18.499998 | 5 |