FastQCFastQC Report
Fri 10 Feb 2017
ERR1632132.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632132.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319060
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8400.26327336551118913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6910.2165736852002758No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT5990.18773898326333605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4950.15514323324766502No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC3830.12004011784617313No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA3620.11345828370839342No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC3400.10656302889738609No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT3350.10499592553124804RNA PCR Primer, Index 17 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGTA200.00183994437.02
CAATCGC200.00183994437.033
TTAGGTT250.005490468329.5999984
GCACTTT250.005490468329.5999986
AAAGGCC400.001928147323.12517
ACATGTT502.6961783E-422.19999929
GGTATCA6650.021.9774441
GCCGTCT603.715435E-521.58333436
GTCACTA450.003819842620.5555571
TTAACGG450.003819842620.55555735
CCCCAGA450.003819842620.5555571
ACTGCGG555.131909E-420.1818188
CACATGT701.2156975E-418.528
GATCAGT609.216891E-418.51
ATACCGC701.2156975E-418.527
ATCAGTA500.00702402118.4999982
CGCATCT500.00702402118.49999815
TAACGGC500.00702402118.49999836
ACATCGT500.00702402118.4999988
TGCACAG500.00702402118.4999985