FastQCFastQC Report
Fri 10 Feb 2017
ERR1632131.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632131.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648460
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3000.028.3666671
TATTAGA601.3359058E-624.6666662
TCGAAAT551.9010009E-523.54545423
GCTATAA400.001930284723.1251
GTCGAAA705.0962662E-621.14285722
CGCTATC450.003824036320.55555717
CGGTCGA555.1397714E-420.18181820
CGAAATA701.21815785E-418.524
ATAATAC801.6149006E-518.53
GTATGTA500.007031657718.4999981
ACGCTTC500.007031657718.49999831
ATAAACG500.007031657718.4999983
CTTATAC4400.017.23863637
GTATCAA4900.016.6122442
TTAGACA803.3797228E-416.18754
TCTTATA7450.015.89261737
TACTACG700.002590860515.8571422
AACTACA700.002590860515.8571422
TAATACT957.052092E-515.5789484
GTATACT855.3596764E-415.2352931
Produced by FastQC (version 0.11.5)