FastQCFastQC Report
Fri 10 Feb 2017
ERR1632130.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632130.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533958
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.11124470463969077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3400.026.6617641
AGTCGGT551.9001864E-523.54545411
GTAGGCG502.6994772E-422.23
ATACCAT450.003823166520.5555556
CTCTATG851.2427536E-619.5882341
TAGGTAA500.007030069418.55
AACGGGT609.227987E-418.57
ATATAGC500.007030069418.53
AATTGAG609.227987E-418.529
TCTAACT500.007030069418.521
CGTAAAT500.007030069418.515
TATACTG500.007030069418.55
GTATCAA4950.017.5656572
GTATTAG852.7186967E-517.4117641
CGGGTTA650.001578435917.0769259
TATTAGA650.001578435917.0769252
GGGCCGT650.001578435917.0769256
TTACCCG650.001578435917.07692513
CAACGGG700.002590056315.8571436
GGTTACC700.002590056315.85714311
Produced by FastQC (version 0.11.5)