##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632130.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	533958
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99710838680196	33.0	31.0	34.0	30.0	34.0
2	32.18155922375917	33.0	31.0	34.0	30.0	34.0
3	32.1912622341083	34.0	31.0	34.0	30.0	34.0
4	35.902261975661006	37.0	35.0	37.0	35.0	37.0
5	35.55476647976058	37.0	35.0	37.0	33.0	37.0
6	35.53728383131258	37.0	35.0	37.0	33.0	37.0
7	35.52730177279861	37.0	35.0	37.0	33.0	37.0
8	35.483187816270195	37.0	35.0	37.0	33.0	37.0
9	37.18412122301754	39.0	37.0	39.0	34.0	39.0
10	36.986219889953894	39.0	37.0	39.0	33.0	39.0
11	37.13366407095689	39.0	37.0	39.0	33.0	39.0
12	37.0620629337888	39.0	37.0	39.0	33.0	39.0
13	37.13413227257575	39.0	37.0	39.0	33.0	39.0
14	38.29944864577364	40.0	38.0	41.0	34.0	41.0
15	38.281741260548586	40.0	38.0	41.0	34.0	41.0
16	38.165883833559946	40.0	38.0	41.0	33.0	41.0
17	38.19723086834545	40.0	38.0	41.0	33.0	41.0
18	38.1715621827934	40.0	38.0	41.0	33.0	41.0
19	38.23365508148581	40.0	38.0	41.0	34.0	41.0
20	38.18647908637009	40.0	38.0	41.0	34.0	41.0
21	38.16042085707115	40.0	38.0	41.0	34.0	41.0
22	38.11523940085175	40.0	38.0	41.0	34.0	41.0
23	38.07497968004974	40.0	38.0	41.0	34.0	41.0
24	37.98779304739324	40.0	37.0	41.0	33.0	41.0
25	37.89086969387105	40.0	37.0	41.0	33.0	41.0
26	37.758973552226955	39.0	37.0	41.0	33.0	41.0
27	37.665505526651906	39.0	37.0	40.0	33.0	41.0
28	37.55839785151641	39.0	37.0	40.0	32.0	41.0
29	37.39247281621401	39.0	37.0	40.0	32.0	41.0
30	37.327894703328724	39.0	36.0	40.0	32.0	41.0
31	37.23963682536829	39.0	36.0	40.0	31.0	41.0
32	37.12510159975129	39.0	36.0	40.0	31.0	41.0
33	36.95354128976437	39.0	36.0	40.0	31.0	41.0
34	36.94326894624671	39.0	36.0	40.0	31.0	41.0
35	36.88128841594283	39.0	36.0	40.0	31.0	41.0
36	36.85523580506332	39.0	36.0	40.0	31.0	41.0
37	36.82414347195847	39.0	35.0	40.0	31.0	41.0
38	36.74798205102274	39.0	35.0	40.0	31.0	41.0
39	36.54306518490218	39.0	35.0	40.0	30.0	41.0
40	36.53199315301953	39.0	35.0	40.0	30.0	41.0
41	36.51864940688219	38.0	35.0	40.0	30.0	41.0
42	36.40187805033354	38.0	35.0	40.0	30.0	41.0
43	35.42496600856247	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	2.0
14	0.0
15	1.0
16	6.0
17	17.0
18	19.0
19	61.0
20	110.0
21	212.0
22	345.0
23	582.0
24	911.0
25	1489.0
26	2111.0
27	3056.0
28	4273.0
29	5847.0
30	8067.0
31	10525.0
32	14381.0
33	19307.0
34	27604.0
35	40130.0
36	61250.0
37	99632.0
38	150700.0
39	83317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.21575854280674	17.432831795759217	12.021544765693182	27.32986489574086
2	20.418085317571794	19.373621146232477	32.524468216601306	27.683825319594423
3	21.190617988680756	20.79545582236805	28.54494173699055	29.46898445196064
4	16.334056236632843	15.041070646005867	32.21657883204297	36.40829428531832
5	17.150412579266533	33.75078189670349	32.42015289592065	16.67865262810933
6	37.2214668569438	32.41734368620753	14.710145741799918	15.651043715048749
7	31.77927852003341	28.159518164350004	19.68244693402852	20.378756381588065
8	29.256046355705877	31.375314163286248	18.710273092640247	20.658366388367625
9	27.792635375816076	13.245798358672406	18.002352244933125	40.9592140205784
10	18.50033148674615	25.11583308050446	31.307144007581122	25.076691425168274
11	38.457144569423065	19.833956977889645	19.78301664175834	21.92588181092895
12	23.97941411122223	23.4070844523352	26.84892819285412	25.764573243588444
13	32.87618127268437	17.730420744702766	22.27871855089726	27.114679431715604
14	24.490128437068083	18.628806010959664	23.09601129676866	33.7850542552036
15	27.535686327389048	25.073320373512527	20.21001651815311	27.180976780945315
16	27.204386861888015	23.682387004221304	21.80901868686301	27.304207447027668
17	26.003543349851487	24.283557882829733	23.361013413039977	26.351885354278803
18	26.160671813138865	23.019975353866784	23.98072507575502	26.838627757239337
19	26.867656257608274	23.178976623629573	23.8013102154102	26.15205690335195
20	26.73318875267343	23.23609722112975	23.584626506204607	26.44608751999221
21	27.79787923394724	22.955925372407567	22.854981103382663	26.391214290262532
22	28.06943617288251	22.935699062473077	22.703658340169078	26.291206424475334
23	27.346720154019604	22.936635465710786	22.707403953119908	27.0092404271497
24	27.080594353863034	23.002370972997877	22.826139883661263	27.090894789477826
25	27.0858382119942	22.557204873791573	23.18197311399024	27.17498380022399
26	26.86185055753449	22.887380655407355	23.405211645859787	26.845557141198373
27	26.921405803452707	23.084025335326	23.18216039463778	26.812408466583516
28	26.51107390468913	22.783439896021783	23.876971597016993	26.82851460227209
29	26.558455908517146	22.96809861449777	23.841388273984094	26.632057203000986
30	26.216106884811165	23.316815180220168	23.593054135343976	26.87402379962469
31	26.676068155173255	23.214559946662472	23.336105086916948	26.773266811247325
32	25.924136355293864	23.339663419220237	23.813670738147945	26.922529487337954
33	26.212361271860335	23.15257005232621	23.885399226156366	26.749669449657087
34	26.61576378666487	22.673131594619804	24.05975750901756	26.651347109697767
35	26.31368010218032	22.840935054817045	24.522527989092772	26.322856853909858
36	26.187265665089765	22.756096921480715	23.947763681787706	27.108873731641815
37	25.52092112113687	22.160731742946073	24.86656253862663	27.451784597290423
38	25.443012371759576	22.214855850085588	25.48477595616135	26.85735582199349
39	24.904767790725113	21.882058139404222	26.185954700556973	27.02721936931369
40	24.659617423093202	21.393442929968277	27.24540132369962	26.701538323238903
41	23.959562362582826	21.33332584210743	27.71791039744699	26.989201397862754
42	23.673022971844226	21.458991156607823	27.772596346529127	27.09538952501882
43	23.119046816416272	20.56098045164601	28.514227710793733	27.805745021143984
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	7.0
2	8.0
3	33.5
4	59.0
5	59.0
6	72.5
7	86.0
8	89.5
9	93.0
10	125.5
11	158.0
12	158.0
13	328.5
14	499.0
15	812.5
16	1126.0
17	1086.0
18	1046.0
19	1046.0
20	1305.0
21	1564.0
22	1664.0
23	1764.0
24	2317.5
25	2871.0
26	2871.0
27	3492.0
28	4113.0
29	5278.0
30	6443.0
31	7821.0
32	9199.0
33	9199.0
34	11290.0
35	13381.0
36	15372.0
37	17363.0
38	19132.5
39	20902.0
40	20902.0
41	23177.5
42	25453.0
43	27430.5
44	29408.0
45	30908.5
46	32409.0
47	32409.0
48	33770.0
49	35131.0
50	36965.5
51	38800.0
52	40159.0
53	41518.0
54	41518.0
55	41963.0
56	42408.0
57	41082.0
58	39756.0
59	38036.5
60	36317.0
61	36317.0
62	34949.5
63	33582.0
64	29624.0
65	25666.0
66	23061.0
67	20456.0
68	20456.0
69	17821.0
70	15186.0
71	13515.5
72	11845.0
73	11107.5
74	10370.0
75	10370.0
76	8911.5
77	7453.0
78	5498.5
79	3544.0
80	2712.5
81	1881.0
82	1881.0
83	1462.5
84	1044.0
85	828.0
86	612.0
87	438.0
88	264.0
89	264.0
90	195.5
91	127.0
92	82.0
93	37.0
94	21.0
95	5.0
96	5.0
97	4.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	533958.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.42113243288539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.69426085166134	69.30958119447465
2	5.7444818605731465	8.779996180503366
3	1.3426863106645843	3.0782882508936145
4	0.6121280993989983	1.871180902002451
5	0.3534038543171429	1.3503761376531258
6	0.2260884014304825	1.0366759000354955
7	0.16344657518329025	0.8743540657448649
8	0.11842713412484614	0.7240268560481558
9	0.08987376628383441	0.618142949587719
>10	0.606007364277194	8.6365049062507
>50	0.03542097008554049	1.8196452267939467
>100	0.013528833213921884	1.789567444504081
>500	2.459787857073429E-4	0.11165998550783385
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	594	0.11124470463969077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	1.8728064754156695E-4
16	0.0	0.0	0.0	1.8728064754156695E-4	3.745612950831339E-4
17	0.0	0.0	0.0	1.8728064754156695E-4	3.745612950831339E-4
18	0.0	0.0	0.0	3.745612950831339E-4	3.745612950831339E-4
19	0.0	0.0	0.0	3.745612950831339E-4	3.745612950831339E-4
20	0.0	0.0	0.0	3.745612950831339E-4	3.745612950831339E-4
21	3.745612950831339E-4	0.0	0.0	5.618419426247008E-4	3.745612950831339E-4
22	3.745612950831339E-4	0.0	0.0	0.0011236838852494017	3.745612950831339E-4
23	3.745612950831339E-4	0.0	0.0	0.0020600871229572363	3.745612950831339E-4
24	3.745612950831339E-4	0.0	0.0	0.0033710516557482048	3.745612950831339E-4
25	3.745612950831339E-4	0.0	0.0	0.005056577483622308	3.745612950831339E-4
26	3.745612950831339E-4	0.0	0.0	0.009551313024619914	3.745612950831339E-4
27	3.745612950831339E-4	0.0	0.0	0.02865393907385974	5.618419426247008E-4
28	3.745612950831339E-4	0.0	0.0	0.09888418190194734	5.618419426247008E-4
29	3.745612950831339E-4	0.0	0.0	0.19364818955798022	5.618419426247008E-4
30	3.745612950831339E-4	0.0	0.0	0.31313324268949994	5.618419426247008E-4
31	3.745612950831339E-4	0.0	0.0	0.6399379726495342	5.618419426247008E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	340	0.0	26.661764	1
AGTCGGT	55	1.9001864E-5	23.545454	11
GTAGGCG	50	2.6994772E-4	22.2	3
ATACCAT	45	0.0038231665	20.555555	6
CTCTATG	85	1.2427536E-6	19.588234	1
TAGGTAA	50	0.0070300694	18.5	5
AACGGGT	60	9.227987E-4	18.5	7
ATATAGC	50	0.0070300694	18.5	3
AATTGAG	60	9.227987E-4	18.5	29
TCTAACT	50	0.0070300694	18.5	21
CGTAAAT	50	0.0070300694	18.5	15
TATACTG	50	0.0070300694	18.5	5
GTATCAA	495	0.0	17.565657	2
GTATTAG	85	2.7186967E-5	17.411764	1
CGGGTTA	65	0.0015784359	17.076925	9
TATTAGA	65	0.0015784359	17.076925	2
GGGCCGT	65	0.0015784359	17.076925	6
TTACCCG	65	0.0015784359	17.076925	13
CAACGGG	70	0.0025900563	15.857143	6
GGTTACC	70	0.0025900563	15.857143	11
>>END_MODULE