FastQCFastQC Report
Fri 10 Feb 2017
ERR1632129.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632129.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6467
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT941.4535333230245864No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC661.020565950208752No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT580.8968609865470852No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC450.6958404205968765No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC350.5412092160197928Illumina PCR Primer Index 7 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG300.463893613731251No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT210.3247255296118757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT190.29379928869645894No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG140.21648368640791713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT100.15463120457708365No Hit
GTCGTAACCAGGCGAGAGTCGCAGTGCTCATCGCTCATAATCC70.10824184320395856No Hit
ATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCT70.10824184320395856RNA PCR Primer, Index 41 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT200.00170958137.037
GCCGTCT200.00170958137.036
TATGCCG250.005105712529.59999833
CATCTCG450.003434578420.55555526
Produced by FastQC (version 0.11.5)