##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632129.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6467
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5006958404206	33.0	31.0	34.0	31.0	34.0
2	32.66027524354415	34.0	31.0	34.0	31.0	34.0
3	32.66305860522653	34.0	31.0	34.0	31.0	34.0
4	36.197000154631205	37.0	35.0	37.0	35.0	37.0
5	36.1059223751353	37.0	35.0	37.0	35.0	37.0
6	36.11226225452296	37.0	35.0	37.0	35.0	37.0
7	36.098190814906445	37.0	35.0	37.0	35.0	37.0
8	36.1039121694758	37.0	35.0	37.0	35.0	37.0
9	37.86500695840421	39.0	38.0	39.0	35.0	39.0
10	37.76016700170094	39.0	37.0	39.0	35.0	39.0
11	37.82418432039586	39.0	38.0	39.0	35.0	39.0
12	37.83500850471625	39.0	37.0	39.0	35.0	39.0
13	37.90985000773156	39.0	38.0	39.0	35.0	39.0
14	39.153548786145045	40.0	39.0	41.0	36.0	41.0
15	39.12293180763878	40.0	38.0	41.0	36.0	41.0
16	39.02102984382248	40.0	38.0	41.0	36.0	41.0
17	39.08040822638009	40.0	38.0	41.0	36.0	41.0
18	39.110097417658885	40.0	39.0	41.0	36.0	41.0
19	39.16158960878305	40.0	39.0	41.0	36.0	41.0
20	39.14890985000773	40.0	39.0	41.0	36.0	41.0
21	39.12154012679759	40.0	39.0	41.0	36.0	41.0
22	39.05412092160198	40.0	38.0	41.0	36.0	41.0
23	39.05721354569352	40.0	39.0	41.0	36.0	41.0
24	38.98840265965672	40.0	38.0	41.0	36.0	41.0
25	38.8994897170249	40.0	38.0	41.0	35.0	41.0
26	38.797587753208596	40.0	38.0	41.0	35.0	41.0
27	38.6862532859131	40.0	38.0	41.0	35.0	41.0
28	38.73109633524045	40.0	38.0	41.0	35.0	41.0
29	38.54677593938457	40.0	38.0	41.0	35.0	41.0
30	38.4898716561002	40.0	38.0	41.0	35.0	41.0
31	38.43404979124787	40.0	38.0	41.0	34.0	41.0
32	38.226225452296276	40.0	38.0	41.0	34.0	41.0
33	38.17334158033091	40.0	38.0	41.0	34.0	41.0
34	38.111025204886346	40.0	38.0	41.0	34.0	41.0
35	38.07685170867481	40.0	38.0	41.0	34.0	41.0
36	38.073759084583266	40.0	37.0	41.0	33.0	41.0
37	38.0074222978197	40.0	37.0	41.0	34.0	41.0
38	37.896242461728775	40.0	37.0	41.0	33.0	41.0
39	37.64960569042833	40.0	37.0	41.0	33.0	41.0
40	37.66939848461419	40.0	37.0	41.0	33.0	41.0
41	37.673418895933196	40.0	37.0	41.0	33.0	41.0
42	37.52249884026597	39.0	36.0	41.0	33.0	41.0
43	36.6268749033555	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	0.0
21	1.0
22	3.0
23	1.0
24	5.0
25	6.0
26	12.0
27	20.0
28	28.0
29	48.0
30	58.0
31	85.0
32	104.0
33	129.0
34	190.0
35	307.0
36	450.0
37	879.0
38	1936.0
39	2204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.89485078088758	17.79805164682233	14.860058759857738	27.44703881243235
2	20.210298438224832	18.679449512911706	33.33848770681924	27.771764342044225
3	18.74130199474254	21.586516158960876	32.70449976805319	26.96768207824339
4	15.092005566723365	16.28266584196691	30.89531467450131	37.73001391680842
5	15.463120457708365	32.194216792948815	33.941549404669864	18.401113344672957
6	32.84366785217257	32.59625792484924	14.396165146126489	20.16390907685171
7	27.493428173805473	29.735580640173186	19.29797433122004	23.473016854801298
8	30.323179217566103	30.15308489253131	18.33926086284212	21.18447502706046
9	25.189423225606927	12.726148136693984	17.566104839956704	44.51832379774238
10	20.875212617906293	23.64311117983609	27.524354414720893	27.957321787536728
11	35.84351322096799	20.117519715478583	19.11241688572754	24.926550177825884
12	21.46281119529921	25.699706200711304	25.127570743776097	27.709911860213392
13	34.25081181382403	17.11767434668316	23.040049481985463	25.59146435750734
14	25.23581258698005	20.04020411319004	21.586516158960876	33.13746714086903
15	30.18401113344673	23.875057986701716	19.328900572135456	26.612030307716093
16	28.142879233029227	23.241070047935676	21.493737436214627	27.12231328282047
17	24.060615432194215	27.493428173805473	21.75661048399567	26.68934591000464
18	25.900726766661514	21.230864388433584	23.612184938920674	29.25622390598423
19	26.070821091696306	24.184320395855885	24.5863615277563	25.15849698469151
20	26.96768207824339	21.045306942941085	23.85959486624401	28.127416112571517
21	30.446884181227773	21.509200556672337	22.761713313746714	25.282201948353176
22	28.869645894541517	24.880160816452758	20.859749497448586	25.39044379155714
23	27.24601824648214	22.158651615896087	22.189577856811503	28.40575228081027
24	26.998608319158805	23.009123241070046	24.431730323179217	25.56053811659193
25	25.405906912014846	22.050409772692127	23.96783670944797	28.57584660584506
26	29.008813978660893	21.416421833926087	23.426627493428175	26.148136693984846
27	24.849234575537345	24.462656564094633	23.813205504870883	26.87490335549714
28	26.13267357352714	21.725684243080252	24.19978351631359	27.941858667079018
29	26.20998917581568	22.88541827740838	24.895623936910468	26.00896860986547
30	25.405906912014846	21.895778568115045	26.11721045306943	26.58110406680068
31	26.68934591000464	22.081336013607547	23.256533168393382	27.972784907994434
32	26.27184165764651	22.715323952373588	26.039894850780886	24.97293953919901
33	24.29256223905984	21.77207360445338	26.086284212154016	27.849079944332765
34	27.23055512602443	22.653471470542755	24.49358280501005	25.62239059842276
35	24.19978351631359	20.983454461110252	29.25622390598423	25.56053811659193
36	25.978042368950057	21.261790629349004	23.6585743002938	29.101592701407142
37	23.8441317457863	21.10715942477192	27.400649451059223	27.648059378382555
38	24.81830833462193	19.406216174424	27.802690582959645	27.972784907994434
39	24.168857275398175	19.715478583578168	28.699551569506728	27.416112571516933
40	21.880315447657335	18.89593319931962	32.10143807020256	27.12231328282047
41	23.16375444564713	19.48353177671254	28.823256533168397	28.529457244471935
42	20.92160197927942	18.385650224215247	33.32302458636153	27.36972321014381
43	19.42167929488171	20.009277872274627	30.864388433585898	29.70465439925777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	1.0
6	2.5
7	4.0
8	4.0
9	4.0
10	6.0
11	8.0
12	8.0
13	11.0
14	14.0
15	24.5
16	35.0
17	31.5
18	28.0
19	28.0
20	32.0
21	36.0
22	24.0
23	12.0
24	12.0
25	12.0
26	12.0
27	21.0
28	30.0
29	30.0
30	30.0
31	37.5
32	45.0
33	45.0
34	57.0
35	69.0
36	81.5
37	94.0
38	132.0
39	170.0
40	170.0
41	201.0
42	232.0
43	280.5
44	329.0
45	393.0
46	457.0
47	457.0
48	529.5
49	602.0
50	594.0
51	586.0
52	628.5
53	671.0
54	671.0
55	609.5
56	548.0
57	644.5
58	741.0
59	663.0
60	585.0
61	585.0
62	497.0
63	409.0
64	332.0
65	255.0
66	219.0
67	183.0
68	183.0
69	151.5
70	120.0
71	94.0
72	68.0
73	54.0
74	40.0
75	40.0
76	31.5
77	23.0
78	20.5
79	18.0
80	10.5
81	3.0
82	3.0
83	2.5
84	2.0
85	2.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6467.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.52110715942477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.56645029039973	87.41301994742538
2	2.886914929962419	5.226534714705427
3	0.18790570550051247	0.510282975104376
4	0.1366586949094636	0.49481985464666767
5	0.01708233686368295	0.07731560228854183
6	0.0	0.0
7	0.0341646737273659	0.21648368640791713
8	0.0	0.0
9	0.0	0.0
>10	0.11957635804578066	2.690582959641256
>50	0.05124701059104886	3.3709602597804236
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	94	1.4535333230245864	No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	66	1.020565950208752	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	58	0.8968609865470852	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC	45	0.6958404205968765	No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC	35	0.5412092160197928	Illumina PCR Primer Index 7 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG	30	0.463893613731251	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21	0.3247255296118757	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.29379928869645894	No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG	14	0.21648368640791713	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.15463120457708365	No Hit
GTCGTAACCAGGCGAGAGTCGCAGTGCTCATCGCTCATAATCC	7	0.10824184320395856	No Hit
ATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCT	7	0.10824184320395856	RNA PCR Primer, Index 41 (95% over 23bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.015463120457708366	0.0
17	0.0	0.0	0.0	0.015463120457708366	0.0
18	0.0	0.0	0.0	0.015463120457708366	0.0
19	0.0	0.0	0.0	0.015463120457708366	0.0
20	0.0	0.0	0.0	0.015463120457708366	0.0
21	0.0	0.0	0.0	0.015463120457708366	0.0
22	0.0	0.0	0.0	0.015463120457708366	0.0
23	0.0	0.0	0.0	0.030926240915416733	0.0
24	0.0	0.0	0.0	0.0463893613731251	0.0
25	0.0	0.0	0.0	0.07731560228854183	0.0
26	0.0	0.0	0.0	0.07731560228854183	0.0
27	0.0	0.0	0.0	0.0927787227462502	0.0
28	0.0	0.0	0.0	0.13916808411937528	0.0
29	0.0	0.0	0.0	0.2319468068656255	0.0
30	0.0	0.0	0.0	0.5721354569352095	0.0
31	0.0	0.0	0.0	1.1751971547858358	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTT	20	0.001709581	37.0	37
GCCGTCT	20	0.001709581	37.0	36
TATGCCG	25	0.0051057125	29.599998	33
CATCTCG	45	0.0034345784	20.555555	26
>>END_MODULE