FastQCFastQC Report
Fri 10 Feb 2017
ERR1632128.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632128.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93479
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG1390.14869649867884766No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA1350.1444174627456434No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC1150.12302228307962215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACT250.005470862629.5999984
GGTATCA508.974968E-625.8999981
TATAGTT400.00191801323.1250025
TGGAGTC450.003799959320.5555555
CCTGTAA555.0946674E-420.1818181
CTTATAC902.1095111E-618.537
TTTATAA801.5909345E-518.52
CAAGATC609.150547E-418.54
TGTAATC609.150547E-418.53
CAAGACA500.006987807318.4999984
ATAGTTA500.006987807318.4999986
TCAAGAT752.041781E-417.2666663
TTATAAA956.94941E-515.5789483
CAACGCA1201.8895516E-615.4166674
ATCCCAG855.2989647E-415.2352947
GTAATCC750.0040670114.84
GCTAGCC750.0040670114.829
AAGATCA750.0040670114.85
AATCCCA1001.0763955E-414.7999996
ATCAACG1252.8888353E-614.7999992
Produced by FastQC (version 0.11.5)