##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632128.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93479
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96521143786305	33.0	31.0	34.0	30.0	34.0
2	32.14983044320115	33.0	31.0	34.0	30.0	34.0
3	32.175312102183376	34.0	31.0	34.0	30.0	34.0
4	35.898993356796716	37.0	35.0	37.0	35.0	37.0
5	35.54725660308733	37.0	35.0	37.0	33.0	37.0
6	35.53757528428845	37.0	35.0	37.0	33.0	37.0
7	35.548583104226616	37.0	35.0	37.0	33.0	37.0
8	35.49184308775233	37.0	35.0	37.0	33.0	37.0
9	37.17631767562768	39.0	37.0	39.0	34.0	39.0
10	36.98845730057018	39.0	37.0	39.0	33.0	39.0
11	37.14248119898587	39.0	37.0	39.0	33.0	39.0
12	37.080777500829065	39.0	37.0	39.0	33.0	39.0
13	37.1426523604232	39.0	37.0	39.0	33.0	39.0
14	38.316734239775776	40.0	38.0	41.0	34.0	41.0
15	38.283079622161125	40.0	38.0	41.0	34.0	41.0
16	38.18897292440013	40.0	38.0	41.0	34.0	41.0
17	38.210624846222146	40.0	38.0	41.0	34.0	41.0
18	38.23816044245232	40.0	38.0	41.0	34.0	41.0
19	38.29890135752415	40.0	38.0	41.0	34.0	41.0
20	38.27494945388804	40.0	38.0	41.0	34.0	41.0
21	38.23261909091882	40.0	38.0	41.0	34.0	41.0
22	38.23163491265418	40.0	38.0	41.0	34.0	41.0
23	38.192813359150186	40.0	38.0	41.0	34.0	41.0
24	38.109864247585016	40.0	38.0	41.0	34.0	41.0
25	38.060184640400514	40.0	37.0	41.0	34.0	41.0
26	37.95114410723264	40.0	37.0	41.0	33.0	41.0
27	37.86977823896276	40.0	37.0	41.0	33.0	41.0
28	37.78011104098247	39.0	37.0	41.0	33.0	41.0
29	37.63957680334621	39.0	37.0	40.0	33.0	41.0
30	37.57865402924721	39.0	37.0	40.0	32.0	41.0
31	37.49282726601697	39.0	37.0	40.0	32.0	41.0
32	37.4023577487992	39.0	37.0	40.0	32.0	41.0
33	37.29649439981172	39.0	36.0	40.0	32.0	41.0
34	37.278287101915936	39.0	36.0	40.0	32.0	41.0
35	37.24540271076926	39.0	36.0	40.0	31.0	41.0
36	37.22540891537137	39.0	36.0	40.0	31.0	41.0
37	37.226607045432665	39.0	36.0	40.0	32.0	41.0
38	37.151285315418434	39.0	36.0	40.0	31.0	41.0
39	36.992811219632216	39.0	36.0	40.0	31.0	41.0
40	37.014516629403396	39.0	36.0	40.0	31.0	41.0
41	37.010355266958356	39.0	36.0	40.0	31.0	41.0
42	36.93423121770665	39.0	36.0	40.0	31.0	41.0
43	36.0156505739257	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	1.0
17	0.0
18	6.0
19	2.0
20	13.0
21	28.0
22	58.0
23	69.0
24	111.0
25	219.0
26	297.0
27	437.0
28	648.0
29	897.0
30	1221.0
31	1737.0
32	2252.0
33	3104.0
34	4494.0
35	6620.0
36	10432.0
37	17456.0
38	27459.0
39	15916.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.666042640593076	19.171150739738337	13.15161694070326	26.01118967896533
2	18.331389937847003	21.211181120893464	35.062420436675616	25.395008504583917
3	19.449288075396613	22.187871072647333	31.35035676462093	27.012484087335125
4	15.264390932722858	16.075268242065064	34.62595877148878	34.03438205372329
5	15.119973469977213	36.39213085291884	34.10177686967126	14.386118807432686
6	34.397030349062355	35.68502016495684	15.355320446303447	14.562629039677361
7	29.314605419399008	30.841151488569622	21.038949924581992	18.805293167449374
8	27.325923469442337	34.26758951208293	20.0408647931621	18.365622225312634
9	26.725788679810435	14.59258229120979	18.799944372532867	39.8816846564469
10	16.5160089431851	27.1911338375464	33.2299232982809	23.062933920987604
11	36.208132307791054	21.384482076188235	21.50215556435135	20.90523005166936
12	22.172894446881116	25.363985494068185	28.920934113544217	23.54218594550648
13	31.19310219407567	19.008547374276574	24.429016142663055	25.36933428898469
14	23.164561024401202	19.957423592464618	24.523154933193553	32.35486044994063
15	25.699889814824722	26.89801987612191	22.034895538035283	25.36719477101809
16	25.504123920880627	26.259373763091176	23.01265524877245	25.223847067255743
17	24.414039516896842	26.12993292611175	24.573433605408702	24.882593951582706
18	23.69730099808513	24.88580322853261	25.958771488783576	25.458124284598682
19	25.22705634420565	25.242032969971866	25.688122466008405	23.842788219814075
20	26.05932883321388	24.384086265364413	25.485938018164507	24.0706468832572
21	25.802586677221623	25.239893452005262	23.79571882454883	25.161801046224287
22	25.614309096160635	25.030220691278256	24.416179034863443	24.939291177697665
23	24.759571668503085	25.398217781533823	24.44934156334578	25.392868986617316
24	25.836818964687257	24.671851431872398	24.798082991901925	24.69324661153842
25	25.466682356465085	24.131623145305362	25.05375538891088	25.347939109318666
26	25.46026380256528	24.205436515153135	24.629061072540356	25.705238609741226
27	24.676130467805603	24.558456979642486	25.00882551161223	25.756587040939678
28	24.644037698306573	25.106173579092626	25.135057071641757	25.114731650959037
29	24.707153478321334	25.481658982231302	25.152173215374575	24.659014324072785
30	24.525294451160153	26.076444976946693	24.621572759657248	24.776687812235902
31	25.200312369623123	25.224916826239046	24.913616962098438	24.661153842039386
32	23.95190363611078	25.300869714053427	25.184265984873612	25.562960664962187
33	24.256784946351587	24.863338289883288	25.54798403919597	25.331892724569155
34	24.407620962997036	24.5702243284588	25.856074626386675	25.16608008215749
35	24.44292300944597	24.486783127761317	25.74054065619016	25.32975320660255
36	24.14873928903818	24.56059649760909	25.832539928754052	25.458124284598682
37	25.0751505685769	23.479070165491713	25.625006685993647	25.82077257993774
38	24.01608917510885	23.659859433669595	27.142994683297854	25.181056707923705
39	23.682324372318917	22.995539105039633	27.486387316937495	25.835749205703955
40	23.183816686100624	22.288428417077633	28.91879459557762	25.608960301244128
41	22.535542742220176	22.37400913574172	29.631254078456127	25.459194043581977
42	22.48633382898833	21.685084350495835	29.990693096845284	25.837888723670556
43	21.262529552091912	21.724665432877973	30.303062719969194	26.709742295060924
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	4.5
4	7.0
5	7.0
6	9.5
7	12.0
8	14.5
9	17.0
10	27.0
11	37.0
12	37.0
13	52.5
14	68.0
15	148.0
16	228.0
17	229.0
18	230.0
19	230.0
20	295.5
21	361.0
22	466.0
23	571.0
24	706.5
25	842.0
26	842.0
27	1004.5
28	1167.0
29	1578.5
30	1990.0
31	2250.5
32	2511.0
33	2511.0
34	2811.5
35	3112.0
36	3408.0
37	3704.0
38	4397.0
39	5090.0
40	5090.0
41	5516.0
42	5942.0
43	5880.0
44	5818.0
45	6128.0
46	6438.0
47	6438.0
48	6742.5
49	7047.0
50	7410.5
51	7774.0
52	8161.5
53	8549.0
54	8549.0
55	7777.0
56	7005.0
57	6564.5
58	6124.0
59	5593.5
60	5063.0
61	5063.0
62	4575.5
63	4088.0
64	3598.5
65	3109.0
66	2683.0
67	2257.0
68	2257.0
69	1863.0
70	1469.0
71	1278.0
72	1087.0
73	903.0
74	719.0
75	719.0
76	563.5
77	408.0
78	356.5
79	305.0
80	235.0
81	165.0
82	165.0
83	124.0
84	83.0
85	66.5
86	50.0
87	32.5
88	15.0
89	15.0
90	13.0
91	11.0
92	7.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	93479.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.44244161790348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.56198324849856	74.3279239187411
2	3.3947375508335353	5.393724793803956
3	0.9749266111874176	2.323516511729907
4	0.48207697072527	1.531894864087121
5	0.34068568042875225	1.353245113875844
6	0.22083972960598963	1.0526428395682452
7	0.15755029490183403	0.87613260732357
8	0.09964719506611727	0.6332973181142288
9	0.10099377878322695	0.722087313728217
>10	0.618081926153349	9.107927983825244
>50	0.04443726266461986	2.2614704906984455
>100	0.004039751151329078	0.41613624450411324
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	139	0.14869649867884766	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	135	0.1444174627456434	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	115	0.12302228307962215	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0010697589833010622	0.0
16	0.0	0.0	0.0	0.0010697589833010622	0.0
17	0.0	0.0	0.0	0.0010697589833010622	0.0
18	0.0	0.0	0.0	0.0010697589833010622	0.0
19	0.0	0.0	0.0	0.0010697589833010622	0.0
20	0.0	0.0	0.0	0.0010697589833010622	0.0
21	0.0	0.0	0.0	0.0010697589833010622	0.0
22	0.0	0.0	0.0	0.003209276949903187	0.0
23	0.0	0.0	0.0	0.005348794916505311	0.0
24	0.0	0.0	0.0	0.005348794916505311	0.0
25	0.0	0.0	0.0	0.006418553899806374	0.0
26	0.0	0.0	0.0	0.00962783084970956	0.0
27	0.0	0.0	0.0	0.02353469763262337	0.0
28	0.0	0.0	0.0	0.08985975459728923	0.0
29	0.0	0.0	0.0	0.20646348377710502	0.0
30	0.0	0.0	0.0	0.3348345617732325	0.0
31	0.0	0.0	0.0	0.7691567089934638	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACT	25	0.0054708626	29.599998	4
GGTATCA	50	8.974968E-6	25.899998	1
TATAGTT	40	0.001918013	23.125002	5
TGGAGTC	45	0.0037999593	20.555555	5
CCTGTAA	55	5.0946674E-4	20.181818	1
CTTATAC	90	2.1095111E-6	18.5	37
TTTATAA	80	1.5909345E-5	18.5	2
CAAGATC	60	9.150547E-4	18.5	4
TGTAATC	60	9.150547E-4	18.5	3
CAAGACA	50	0.0069878073	18.499998	4
ATAGTTA	50	0.0069878073	18.499998	6
TCAAGAT	75	2.041781E-4	17.266666	3
TTATAAA	95	6.94941E-5	15.578948	3
CAACGCA	120	1.8895516E-6	15.416667	4
ATCCCAG	85	5.2989647E-4	15.235294	7
GTAATCC	75	0.00406701	14.8	4
GCTAGCC	75	0.00406701	14.8	29
AAGATCA	75	0.00406701	14.8	5
AATCCCA	100	1.0763955E-4	14.799999	6
ATCAACG	125	2.8888353E-6	14.799999	2
>>END_MODULE