FastQCFastQC Report
Fri 10 Feb 2017
ERR1632127.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632127.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87173
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT9531.093228407878586RNA PCR Primer, Index 29 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT8690.9968682963761716No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC4880.5598063620616476RNA PCR Primer, Index 29 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC4720.5414520551088067No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG3570.4095304738852627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.24893028804790474No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC2110.24204742294058942RNA PCR Primer, Index 29 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1990.22828169272595872No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG1660.19042593463572435No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1530.17551306023654115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC502.6722955E-729.637
GGTATCA453.952662E-628.7777791
TATACAC900.028.7777793
GCCGTCT1550.026.25806436
CCGTCTT1600.025.43750237
TACACAT1053.8198777E-1122.904765
GGGACAG450.003797928820.5555575
AGCACTG450.003797928820.5555575
ACTGGGC555.0908694E-420.1818188
TTATACA1202.2919266E-1020.0416682
ATGCCGT2100.019.3809534
TGCCGTC2100.019.3809535
GTATCAA1152.9376679E-919.3043481
CTTATAC1451.4733814E-1017.862071
ATACACA1451.4733814E-1017.862074
TATGCCG2350.017.31914933
GGCTGGA956.9407935E-515.5789481
ATCAACG1455.1524694E-815.3103463
CTCTTAT1601.0504664E-815.03125137
TCTTATA908.1663736E-414.38888935
Produced by FastQC (version 0.11.5)