##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632127.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	87173
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3653424798963	33.0	31.0	34.0	31.0	34.0
2	32.546338889335	34.0	31.0	34.0	31.0	34.0
3	32.56192857880307	34.0	31.0	34.0	31.0	34.0
4	36.13788673098322	37.0	35.0	37.0	35.0	37.0
5	35.9714016954791	37.0	35.0	37.0	35.0	37.0
6	35.98528214011219	37.0	35.0	37.0	35.0	37.0
7	35.97810101751689	37.0	35.0	37.0	35.0	37.0
8	35.94443233570028	37.0	35.0	37.0	35.0	37.0
9	37.67230679224072	39.0	37.0	39.0	35.0	39.0
10	37.54253037064229	39.0	37.0	39.0	35.0	39.0
11	37.67624149679373	39.0	37.0	39.0	35.0	39.0
12	37.61936608812361	39.0	37.0	39.0	35.0	39.0
13	37.665343627040485	39.0	37.0	39.0	35.0	39.0
14	38.9265483578631	40.0	38.0	41.0	36.0	41.0
15	38.9369300127333	40.0	38.0	41.0	36.0	41.0
16	38.84403427666823	40.0	38.0	41.0	36.0	41.0
17	38.85690523441892	40.0	38.0	41.0	36.0	41.0
18	38.89664230897181	40.0	38.0	41.0	36.0	41.0
19	38.947816411044705	40.0	38.0	41.0	36.0	41.0
20	38.952175558946	40.0	38.0	41.0	36.0	41.0
21	38.93164167804251	40.0	38.0	41.0	36.0	41.0
22	38.88322072201255	40.0	38.0	41.0	35.0	41.0
23	38.88725866954218	40.0	38.0	41.0	35.0	41.0
24	38.820185149071385	40.0	38.0	41.0	35.0	41.0
25	38.735502965367715	40.0	38.0	41.0	35.0	41.0
26	38.65897697681621	40.0	38.0	41.0	35.0	41.0
27	38.5386530232985	40.0	38.0	41.0	35.0	41.0
28	38.52136556043729	40.0	38.0	41.0	35.0	41.0
29	38.39957326236335	40.0	38.0	41.0	34.0	41.0
30	38.35855138632375	40.0	38.0	41.0	34.0	41.0
31	38.25153430534684	40.0	38.0	41.0	34.0	41.0
32	38.161988230300665	40.0	38.0	41.0	34.0	41.0
33	38.028724490381194	40.0	38.0	41.0	33.0	41.0
34	38.04239844906106	40.0	38.0	41.0	34.0	41.0
35	38.03535498376791	40.0	38.0	41.0	34.0	41.0
36	37.97714888784371	40.0	38.0	41.0	33.0	41.0
37	37.96751287669347	40.0	38.0	41.0	33.0	41.0
38	37.9165452605738	40.0	37.0	41.0	33.0	41.0
39	37.739529441455495	40.0	37.0	41.0	33.0	41.0
40	37.739127940990905	40.0	37.0	41.0	33.0	41.0
41	37.77671985591869	40.0	37.0	41.0	33.0	41.0
42	37.635644064102415	40.0	37.0	41.0	33.0	41.0
43	36.81134066740849	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	7.0
21	13.0
22	29.0
23	60.0
24	85.0
25	123.0
26	174.0
27	272.0
28	406.0
29	619.0
30	772.0
31	1148.0
32	1514.0
33	2055.0
34	2915.0
35	4157.0
36	6795.0
37	12330.0
38	26662.0
39	27029.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.77493031099078	19.808885778853544	13.83455886570383	26.58162504445184
2	18.196001055372648	20.52470375001434	35.19553072625698	26.08376446835603
3	19.029974877542358	22.27983435237975	32.0833285535659	26.606862216511995
4	14.374863776628086	16.864166657107134	33.19720555676643	35.56376400949836
5	14.883048650384866	34.983309052114755	34.551982838723	15.581659458777375
6	33.89696350934349	34.8250031546465	15.702109598155392	15.57592373785461
7	28.04079244720269	30.561068220664655	20.822961237998	20.575178094134653
8	27.482133229325594	32.95401098964128	20.357220699069668	19.20663508196345
9	25.16834340908309	14.302593693001272	19.8800087182958	40.64905417961984
10	16.93184816399573	26.901678271941996	32.872563752538056	23.29390981152421
11	34.368439769194595	21.024858614479253	21.713145125210787	22.89355649111537
12	21.23363885606782	26.39119910981611	27.997200968189688	24.377961065926375
13	31.024514471223892	19.111422114645592	23.552017253048536	26.312046161081987
14	23.032360937446228	21.449301962763702	24.202448005689835	31.315889094100235
15	25.02150895346036	27.573904764089797	21.75903089259289	25.64555538985695
16	26.58162504445184	25.526252394663484	23.15510536519335	24.737017195691326
17	22.197239971091967	28.605187386002545	24.86435020017666	24.33322244272883
18	24.21850802427357	24.387138219402797	25.961020040608908	25.433333715714728
19	23.794064675989123	25.530840971401698	27.237791518015896	23.43730283459328
20	25.284204971722897	24.866644488545766	25.264703520585503	24.584447019145834
21	25.08919046034896	24.64983423766533	25.042157548782306	25.2188177532034
22	25.135076227731062	27.541784726922323	24.431876842600346	22.891262202746262
23	25.455129455221225	24.21391944753536	24.7932272607344	25.537723836509013
24	24.9262959861425	25.175226274190404	25.3977722459936	24.5007054936735
25	24.896470237344133	25.51478095281796	25.767152673419524	23.821596136418385
26	24.179505121998783	25.809597008247962	25.38974223670173	24.621155633051515
27	23.62887591341356	26.898236839388346	26.08491161254058	23.387975634657522
28	23.703440285409474	24.380255354295482	26.635540821125808	25.280763539169236
29	22.914205086437313	25.31861929725947	26.791552430224957	24.97562318607826
30	24.683101419017355	25.08804331616441	26.967065490461493	23.26178977435674
31	23.743590331868813	25.48036662728138	24.728987186399458	26.047055854450345
32	23.62084590412169	26.081470179986923	26.6091565048811	23.68852741101029
33	23.430419969485968	24.997418925584757	27.36283023413213	24.20933087079715
34	23.368474183520128	24.950386014018104	26.523120691039658	25.158019111422114
35	22.718043430878826	23.893866220045197	29.840661672765652	23.547428676310325
36	24.142796508093102	25.239466348525347	25.79353698966423	24.82420015371732
37	22.913057942252763	24.079703577942713	27.748270680141786	25.25896779966274
38	22.65609764491299	23.196402555837242	28.672868892891145	25.47463090635862
39	23.170018239592537	23.094306723412068	28.18992119119452	25.545753845800878
40	21.352941851261285	22.154795636263522	31.946818395604144	24.54544411687105
41	22.169708510662705	22.511557477659366	28.869030548449636	26.449703463228293
42	21.325410390832023	22.641184770513807	31.225264703520583	24.808140135133584
43	20.551088066259048	21.955192548151377	31.356039140559577	26.137680245029998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	2.5
2	2.0
3	10.5
4	19.0
5	19.0
6	36.0
7	53.0
8	51.5
9	50.0
10	67.0
11	84.0
12	84.0
13	170.5
14	257.0
15	366.0
16	475.0
17	453.0
18	431.0
19	431.0
20	538.0
21	645.0
22	658.0
23	671.0
24	776.5
25	882.0
26	882.0
27	1106.0
28	1330.0
29	1590.5
30	1851.0
31	2112.0
32	2373.0
33	2373.0
34	2782.0
35	3191.0
36	3582.5
37	3974.0
38	4280.0
39	4586.0
40	4586.0
41	4936.5
42	5287.0
43	5466.5
44	5646.0
45	5678.5
46	5711.0
47	5711.0
48	5848.5
49	5986.0
50	6582.5
51	7179.0
52	6623.5
53	6068.0
54	6068.0
55	6798.0
56	7528.0
57	6993.5
58	6459.0
59	5577.5
60	4696.0
61	4696.0
62	4270.0
63	3844.0
64	3296.5
65	2749.0
66	2341.5
67	1934.0
68	1934.0
69	1563.0
70	1192.0
71	980.0
72	768.0
73	635.0
74	502.0
75	502.0
76	417.0
77	332.0
78	260.5
79	189.0
80	145.0
81	101.0
82	101.0
83	82.0
84	63.0
85	50.0
86	37.0
87	25.5
88	14.0
89	14.0
90	10.5
91	7.0
92	5.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	87173.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.77104149220516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.44266430135544	74.86033519553072
2	7.001690827951326	11.590744840719031
3	1.4815533442359399	3.6788913998600488
4	0.48091581894281676	1.5922361281589483
5	0.2231338525930648	0.923451068564808
6	0.11780358677273609	0.5850435341218038
7	0.06929622751337418	0.4015004645933948
8	0.0332621892064196	0.22025168343409082
9	0.03187626465615212	0.23745884620237917
>10	0.10255841671979378	1.148291328737109
>50	0.0013859245502674835	0.07571151618046872
>100	0.011087396402139868	2.5959872896424354
>500	0.002771849100534967	2.090096704254758
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	953	1.093228407878586	RNA PCR Primer, Index 29 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	869	0.9968682963761716	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	488	0.5598063620616476	RNA PCR Primer, Index 29 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	472	0.5414520551088067	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	357	0.4095304738852627	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	217	0.24893028804790474	No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC	211	0.24204742294058942	RNA PCR Primer, Index 29 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	199	0.22828169272595872	No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG	166	0.19042593463572435	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	153	0.17551306023654115	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002294288369105113	0.0
2	0.0	0.0	0.0	0.002294288369105113	0.0
3	0.0	0.0	0.0	0.002294288369105113	0.0
4	0.0	0.0	0.0	0.002294288369105113	0.0
5	0.0	0.0	0.0	0.002294288369105113	0.0
6	0.0	0.0	0.0	0.002294288369105113	0.0
7	0.0	0.0	0.0	0.002294288369105113	0.0
8	0.0	0.0	0.0	0.002294288369105113	0.0
9	0.0	0.0	0.0	0.003441432553657669	0.0
10	0.0	0.0	0.0	0.004588576738210226	0.0
11	0.0	0.0	0.0	0.004588576738210226	0.0
12	0.0	0.0	0.0	0.004588576738210226	0.0
13	0.0	0.0	0.0	0.004588576738210226	0.0
14	0.0	0.0	0.0	0.004588576738210226	0.0
15	0.0	0.0	0.0	0.004588576738210226	0.0
16	0.0	0.0	0.0	0.004588576738210226	0.0
17	0.0	0.0	0.0	0.004588576738210226	0.0
18	0.0	0.0	0.0	0.004588576738210226	0.0
19	0.0	0.0	0.0	0.004588576738210226	0.0
20	0.0	0.0	0.0	0.004588576738210226	0.0
21	0.0	0.0	0.0	0.004588576738210226	0.0
22	0.0	0.0	0.0	0.004588576738210226	0.0
23	0.0	0.0	0.0	0.005735720922762782	0.0
24	0.0	0.0	0.0	0.006882865107315338	0.0
25	0.0	0.0	0.0	0.013765730214630676	0.0
26	0.0	0.0	0.0	0.01606001858373579	0.0
27	0.0	0.0	0.0	0.03326718135202414	0.0
28	0.0	0.0	0.0	0.09291867894875706	0.0
29	0.0	0.0	0.0	0.1651887625755681	0.0
30	0.0	0.0	0.0	0.30399320890642745	0.0
31	0.0	0.0	0.0	0.7066408176843747	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	50	2.6722955E-7	29.6	37
GGTATCA	45	3.952662E-6	28.777779	1
TATACAC	90	0.0	28.777779	3
GCCGTCT	155	0.0	26.258064	36
CCGTCTT	160	0.0	25.437502	37
TACACAT	105	3.8198777E-11	22.90476	5
GGGACAG	45	0.0037979288	20.555557	5
AGCACTG	45	0.0037979288	20.555557	5
ACTGGGC	55	5.0908694E-4	20.181818	8
TTATACA	120	2.2919266E-10	20.041668	2
ATGCCGT	210	0.0	19.38095	34
TGCCGTC	210	0.0	19.38095	35
GTATCAA	115	2.9376679E-9	19.304348	1
CTTATAC	145	1.4733814E-10	17.86207	1
ATACACA	145	1.4733814E-10	17.86207	4
TATGCCG	235	0.0	17.319149	33
GGCTGGA	95	6.9407935E-5	15.578948	1
ATCAACG	145	5.1524694E-8	15.310346	3
CTCTTAT	160	1.0504664E-8	15.031251	37
TCTTATA	90	8.1663736E-4	14.388889	35
>>END_MODULE