FastQCFastQC Report
Fri 10 Feb 2017
ERR1632126.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632126.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6889904
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT141370.20518428123236548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT119060.1728035688160532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT103090.14962472626614245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35400.027.0706231
CTTATAC85200.018.2611537
TCTAGAC16900.017.0769213
GTATCAA56050.016.8331852
CTAGACA20000.016.2799994
GTCTAGA15650.016.194891
GTTCTAG18650.016.16891
TAGACAT20300.014.854685
GCAATTA15700.014.61146517
TAGAACT20050.014.4862844
CTAGAAC18300.014.4562843
ACTGATC16300.014.4141118
TCTTATA139800.014.26537837
CTCTAGT18400.014.17663127
TATACAC27800.013.44244637
TTCTAGA23750.013.322
AATACGC2551.6370905E-1113.0588245
CGAATTA3300.012.89393915
ACATGTA16550.012.8549858
TTATACA45950.012.68226337
Produced by FastQC (version 0.11.5)