##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632126.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6889904
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.754972057665825	34.0	31.0	34.0	31.0	34.0
2	32.90700378408756	34.0	31.0	34.0	31.0	34.0
3	32.91631218664295	34.0	33.0	34.0	31.0	34.0
4	36.36844998130598	37.0	37.0	37.0	35.0	37.0
5	36.28071523202645	37.0	37.0	37.0	35.0	37.0
6	36.31488580972972	37.0	37.0	37.0	35.0	37.0
7	36.30810443802991	37.0	37.0	37.0	35.0	37.0
8	36.29055774942583	37.0	37.0	37.0	35.0	37.0
9	38.06978790996217	39.0	38.0	39.0	37.0	39.0
10	37.99972408904391	39.0	38.0	39.0	35.0	39.0
11	38.11034391770916	39.0	38.0	39.0	37.0	39.0
12	38.08221928781591	39.0	38.0	39.0	37.0	39.0
13	38.10208879543169	39.0	38.0	39.0	37.0	39.0
14	39.50450644885618	40.0	39.0	41.0	37.0	41.0
15	39.52470905254994	40.0	39.0	41.0	37.0	41.0
16	39.41645863280533	40.0	39.0	41.0	37.0	41.0
17	39.46577630109215	40.0	39.0	41.0	37.0	41.0
18	39.48826964787899	41.0	39.0	41.0	37.0	41.0
19	39.540922921422414	41.0	39.0	41.0	37.0	41.0
20	39.51468801306956	41.0	39.0	41.0	37.0	41.0
21	39.48863104623809	41.0	39.0	41.0	37.0	41.0
22	39.47149684523906	41.0	39.0	41.0	37.0	41.0
23	39.43535396139046	40.0	39.0	41.0	37.0	41.0
24	39.38409577259712	41.0	39.0	41.0	37.0	41.0
25	39.32624968359501	40.0	39.0	41.0	36.0	41.0
26	39.24676149333866	40.0	39.0	41.0	36.0	41.0
27	39.17996375566336	40.0	39.0	41.0	36.0	41.0
28	39.1204125630778	40.0	39.0	41.0	36.0	41.0
29	38.98457859499929	40.0	39.0	41.0	35.0	41.0
30	38.94206552079681	40.0	39.0	41.0	35.0	41.0
31	38.87094145288526	40.0	38.0	41.0	35.0	41.0
32	38.80420496424914	40.0	38.0	41.0	35.0	41.0
33	38.668703801968796	40.0	38.0	41.0	35.0	41.0
34	38.66899059841763	40.0	38.0	41.0	35.0	41.0
35	38.64933720411779	40.0	38.0	41.0	35.0	41.0
36	38.62467764427487	40.0	38.0	41.0	35.0	41.0
37	38.59397257204164	40.0	38.0	41.0	35.0	41.0
38	38.53104528016646	40.0	38.0	41.0	35.0	41.0
39	38.347689895243825	40.0	38.0	41.0	34.0	41.0
40	38.35012722963919	40.0	38.0	41.0	34.0	41.0
41	38.34914593875328	40.0	38.0	41.0	34.0	41.0
42	38.20922323446016	40.0	38.0	41.0	34.0	41.0
43	37.389048236376006	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	9.0
11	6.0
12	2.0
13	6.0
14	10.0
15	6.0
16	16.0
17	36.0
18	91.0
19	172.0
20	332.0
21	654.0
22	1266.0
23	2271.0
24	3839.0
25	6170.0
26	9500.0
27	13826.0
28	20493.0
29	29535.0
30	41873.0
31	57777.0
32	79283.0
33	110149.0
34	162194.0
35	243572.0
36	395087.0
37	739818.0
38	1714932.0
39	3256974.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.59790789537851	17.58369927940941	11.73962656083452	27.078766264377556
2	19.249789256860474	19.816328355228173	33.67501201758399	27.258870370327365
3	20.44696123487352	20.70068029975454	29.111174843655295	29.741183621716644
4	15.319865124390702	14.943270617413537	32.962781484328374	36.774082773867384
5	16.501202338958567	34.21218931352309	32.72849665249327	16.55811169502507
6	36.233422120250154	33.09063522510618	15.145798838416328	15.530143816227337
7	31.040403465708664	29.056253904263396	19.924138275366392	19.979204354661544
8	28.047778314472886	32.044394232488585	19.44170775093528	20.466119702103253
9	27.145675759778364	13.773355913231883	19.08216718259064	39.99880114439911
10	17.723019072544407	25.98506452339539	32.06343658779571	24.228479816264496
11	37.187629900213416	20.517281518000832	20.71712465079339	21.57796393099236
12	23.267653656712778	24.012758378055775	27.96112979222933	24.758458173002122
13	31.840995172066254	18.71493419937346	23.22874164864997	26.215328979910314
14	23.926574303502633	19.423289497212153	24.040741351403444	32.60939484788177
15	26.37427168796546	26.095298279917976	21.40279458175324	26.12763545036331
16	26.236490377804973	24.744931134018703	22.883686042650233	26.13489244552609
17	24.84583239476196	25.2210480726582	24.468570244229817	25.464549288350025
18	25.32006251465913	23.9453554069839	25.10692456672836	25.62765751162861
19	25.728674884294467	24.109610235498202	25.055385387082318	25.106329493125013
20	25.933641455671953	24.220076215865998	24.942814878117314	24.903467450344735
21	26.30313862137992	23.99912974114008	24.60714982385821	25.09058181362179
22	26.55597233285108	23.994267554381018	23.931639686126253	25.518120426641648
23	25.994919522826443	24.034006859892386	24.31234165236555	25.65873196491562
24	25.733740266918087	23.897023819199802	24.15621465843356	26.213021255448552
25	26.10465980367796	23.665511159516882	24.42957115222505	25.800257884580102
26	25.76278276155952	23.65675922335057	24.557845798722305	26.0226122163676
27	25.295824150815456	23.981872606642995	24.674799532765622	26.047503709775928
28	25.348292225842332	23.939085363163258	24.77789240604804	25.934730004946367
29	25.401805308172655	24.26051219291299	24.525740852122176	25.811941646792175
30	25.07239578374387	24.34020851379061	24.72375522213372	25.8636404803318
31	25.733333875188976	24.09204830720428	24.44555105557349	25.72906676203326
32	24.949563883618698	24.03087183798207	24.946399833727728	26.07316444467151
33	25.07630004714144	23.69401663651627	25.25943757706929	25.970245739273
34	25.557758134220737	23.096098291064724	25.315156785929094	26.030986788785448
35	24.833350363081983	23.296725179334864	25.943554511064303	25.926369946518847
36	25.150016604004932	22.550154544968986	26.106953014149397	26.192875836876684
37	24.618049830592707	22.1166942238963	26.541342230602922	26.723913714908075
38	24.041423508948746	22.061874882436676	27.562648187841226	26.33405342077335
39	23.818387019615948	21.946561229300148	28.111030284311656	26.124021466772252
40	23.437568941454046	22.20814687693762	28.343616979278668	26.010667202329667
41	23.283372308235354	21.567542885938614	29.069505177430628	26.079579628395404
42	22.676527858733593	21.92210515560159	29.098518644091413	26.30284834157341
43	21.913396761406254	21.65294320501418	29.398392198207695	27.035267835371872
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	563.0
1	687.5
2	812.0
3	1779.0
4	2746.0
5	2746.0
6	3534.5
7	4323.0
8	4180.5
9	4038.0
10	5702.0
11	7366.0
12	7366.0
13	11458.5
14	15551.0
15	22475.0
16	29399.0
17	29133.0
18	28867.0
19	28867.0
20	34616.5
21	40366.0
22	40574.5
23	40783.0
24	48901.0
25	57019.0
26	57019.0
27	67535.5
28	78052.0
29	91647.5
30	105243.0
31	122426.5
32	139610.0
33	139610.0
34	165673.0
35	191736.0
36	219195.0
37	246654.0
38	267685.0
39	288716.0
40	288716.0
41	315466.5
42	342217.0
43	369723.0
44	397229.0
45	425830.0
46	454431.0
47	454431.0
48	469244.0
49	484057.0
50	498730.0
51	513403.0
52	517240.5
53	521078.0
54	521078.0
55	513983.5
56	506889.0
57	491741.5
58	476594.0
59	465098.5
60	453603.0
61	453603.0
62	426601.0
63	399599.0
64	364020.0
65	328441.0
66	288780.0
67	249119.0
68	249119.0
69	212528.5
70	175938.0
71	149330.0
72	122722.0
73	98661.0
74	74600.0
75	74600.0
76	59422.0
77	44244.0
78	35420.5
79	26597.0
80	22204.5
81	17812.0
82	17812.0
83	13536.0
84	9260.0
85	7366.5
86	5473.0
87	4194.5
88	2916.0
89	2916.0
90	2130.0
91	1344.0
92	836.0
93	328.0
94	214.5
95	101.0
96	101.0
97	78.0
98	55.0
99	32.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6889904.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.46724287490781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77223846182004	38.966454517910066
2	11.721420823702339	11.59652741450171
3	3.6882406348629453	5.473412857976085
4	1.6967042009274877	3.3572511517662567
5	0.9435177965620444	2.3336611999666257
6	0.6134644101680836	1.8207835783737993
7	0.4138957433768602	1.4331996883759561
8	0.3032042066893855	1.1998940906398063
9	0.22734510935905677	1.0121522166978245
>10	1.3696052360423863	12.778155611915967
>50	0.13287120389091334	4.608141987230708
>100	0.10665024799518857	10.401779896825127
>500	0.00857341789776154	2.8987526698530734
>1k	0.0021506622016557115	1.5169667282100079
>5k	2.9461125963000153E-5	0.07308663777000604
>10k+	8.838337788900047E-5	0.5297797519870906
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14137	0.20518428123236548	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11906	0.1728035688160532	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10309	0.14962472626614245	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	4.354197097666383E-5	0.0	1.4513990325554609E-5	1.4513990325554609E-5
2	0.0	1.451399032555461E-4	0.0	4.354197097666383E-5	1.4513990325554609E-5
3	0.0	1.886818742322099E-4	0.0	7.256995162777305E-5	1.4513990325554609E-5
4	0.0	2.3222384520887374E-4	0.0	1.3062591292999147E-4	1.4513990325554609E-5
5	0.0	4.2090571944108365E-4	0.0	1.451399032555461E-4	1.4513990325554609E-5
6	0.0	4.2090571944108365E-4	0.0	1.451399032555461E-4	4.354197097666383E-5
7	0.0	4.3541970976663824E-4	0.0	1.5965389358110069E-4	4.354197097666383E-5
8	0.0	4.3541970976663824E-4	0.0	1.5965389358110069E-4	4.354197097666383E-5
9	0.0	4.934756710688567E-4	0.0	2.757658161855376E-4	4.354197097666383E-5
10	0.0	5.370176420455205E-4	0.0	5.660456226966298E-4	4.354197097666383E-5
11	0.0	6.386155743244027E-4	0.0	6.531295646499573E-4	4.354197097666383E-5
12	0.0	6.386155743244027E-4	0.0	7.256995162777304E-4	4.354197097666383E-5
13	0.0	6.386155743244027E-4	0.0	7.256995162777304E-4	4.354197097666383E-5
14	0.0	6.531295646499573E-4	0.0	7.256995162777304E-4	4.354197097666383E-5
15	0.0	6.531295646499573E-4	0.0	7.256995162777304E-4	5.8055961302218436E-5
16	0.0	7.111855259521758E-4	0.0	7.692414872543942E-4	1.0159793227888226E-4
17	0.0	7.256995162777304E-4	0.0	8.853534098588311E-4	1.0159793227888226E-4
18	0.0	8.127834582310581E-4	0.0	9.143813905099404E-4	1.3062591292999147E-4
19	0.0	8.418114388821673E-4	0.0	0.001001465332463268	1.3062591292999147E-4
20	0.0	8.418114388821673E-4	0.0	0.0012046611970210326	1.451399032555461E-4
21	0.0	8.418114388821673E-4	0.0	0.001407857061578797	1.886818742322099E-4
22	0.0	8.418114388821673E-4	0.0	0.00185779076167099	1.886818742322099E-4
23	0.0	0.0010595212937654864	0.0	0.0027576581618553755	1.886818742322099E-4
24	0.0	0.0011611192260443687	0.0	0.004731560846130803	1.886818742322099E-4
25	0.0	0.0011611192260443687	0.0	0.00686511742398733	1.886818742322099E-4
26	0.0	0.0011611192260443687	0.0	0.011770846154024787	2.0319586455776453E-4
27	0.0	0.001190147206695478	0.0	0.04650282500307697	2.1770985488331912E-4
28	0.0	0.001335287109951024	0.0	0.15831860647114968	2.3222384520887374E-4
29	0.0	0.00148042701320657	0.0	0.31353121901263065	2.3222384520887374E-4
30	0.0	0.00148042701320657	0.0	0.5301089826505565	2.3222384520887374E-4
31	0.0	0.00148042701320657	0.0	1.151728674303735	2.3222384520887374E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3540	0.0	27.070623	1
CTTATAC	8520	0.0	18.26115	37
TCTAGAC	1690	0.0	17.076921	3
GTATCAA	5605	0.0	16.833185	2
CTAGACA	2000	0.0	16.279999	4
GTCTAGA	1565	0.0	16.19489	1
GTTCTAG	1865	0.0	16.1689	1
TAGACAT	2030	0.0	14.85468	5
GCAATTA	1570	0.0	14.611465	17
TAGAACT	2005	0.0	14.486284	4
CTAGAAC	1830	0.0	14.456284	3
ACTGATC	1630	0.0	14.414111	8
TCTTATA	13980	0.0	14.265378	37
CTCTAGT	1840	0.0	14.176631	27
TATACAC	2780	0.0	13.442446	37
TTCTAGA	2375	0.0	13.32	2
AATACGC	255	1.6370905E-11	13.058824	5
CGAATTA	330	0.0	12.893939	15
ACATGTA	1655	0.0	12.854985	8
TTATACA	4595	0.0	12.682263	37
>>END_MODULE