FastQCFastQC Report
Fri 10 Feb 2017
ERR1632125.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632125.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518889
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24100.1586686058033207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18300.12048280025729333No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17190.11317482712693291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8650.027.3757211
CATACCG450.003826365320.5555575
AAGACCG555.144139E-420.1818185
CTTATAC19400.019.83505237
CGGTACT752.0678181E-417.2666664
GTCTATA650.001580250617.0769231
GTCGTTG1554.0199666E-1016.7096791
GTATCAA14600.016.219182
TATGCCG2200.015.97727333
GTACTAG1059.346977E-615.8571421
GTATTAG2650.015.3584911
TCTTATA31950.015.28638437
TAGCACT2201.8189894E-1215.1363644
ATAATAC1508.105599E-814.83
TTCGGTC750.004105214514.828
CGTATGC2400.014.64583331
TGCCGTC2550.014.50980435
GTATATG1956.730261E-1014.230771
TGGACTA1304.4459393E-614.2307695
TTAGAAC1854.9003575E-914.03
Produced by FastQC (version 0.11.5)