FastQCFastQC Report
Fri 10 Feb 2017
ERR1632124.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632124.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289309
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25030.19413499789422084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21040.16318818840169425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16120.1250282127868494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7800.026.5641041
CGCGCTT400.001931314223.12512
GCGGATA500.00703533718.49999829
TATACCG500.00703533718.4999985
TATGCCG3150.018.2063533
CCGTCTT3300.017.37878837
ATGCCGT3300.017.37878834
GCCGTCT3300.017.37878836
TTATACT1603.45608E-1117.343754
GTATCAA12100.016.9710752
CGTATGC3400.016.86764731
CTCGTAT3550.016.1549329
TCGTATG3600.015.93055530
ACCGAAC700.002592723115.8571423
GTTGTCG750.00410476514.80000121
TGCCGTC4000.014.79999935
ATGCGTT1152.2101463E-514.47826136
CGTTTAG1152.2101463E-514.47826126
TCTCGTA4050.014.16049428
CTTATAC9150.014.15300637
Produced by FastQC (version 0.11.5)