##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632124.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1289309
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.472731517425224	34.0	31.0	34.0	31.0	34.0
2	32.64014212264089	34.0	31.0	34.0	31.0	34.0
3	32.655860619913454	34.0	31.0	34.0	31.0	34.0
4	36.20187712953218	37.0	37.0	37.0	35.0	37.0
5	36.05009892896117	37.0	35.0	37.0	35.0	37.0
6	36.07589569296422	37.0	35.0	37.0	35.0	37.0
7	36.07268311940737	37.0	35.0	37.0	35.0	37.0
8	36.05021914839654	37.0	35.0	37.0	35.0	37.0
9	37.76772208989466	39.0	37.0	39.0	35.0	39.0
10	37.67628318735074	39.0	37.0	39.0	35.0	39.0
11	37.79024423160003	39.0	37.0	39.0	35.0	39.0
12	37.75622213138976	39.0	37.0	39.0	35.0	39.0
13	37.78213678800039	39.0	37.0	39.0	35.0	39.0
14	39.12001700135499	40.0	38.0	41.0	36.0	41.0
15	39.134075694810164	40.0	38.0	41.0	36.0	41.0
16	39.017232486549	40.0	38.0	41.0	36.0	41.0
17	39.06556768005187	40.0	38.0	41.0	36.0	41.0
18	39.08137304556161	40.0	38.0	41.0	36.0	41.0
19	39.13808792151455	40.0	39.0	41.0	36.0	41.0
20	39.12503519327019	40.0	39.0	41.0	36.0	41.0
21	39.097556908390466	40.0	39.0	41.0	36.0	41.0
22	39.07924322253238	40.0	39.0	41.0	36.0	41.0
23	39.047495208673794	40.0	39.0	41.0	36.0	41.0
24	39.00148296490601	40.0	38.0	41.0	36.0	41.0
25	38.94045647707415	40.0	38.0	41.0	35.0	41.0
26	38.84822257503826	40.0	38.0	41.0	35.0	41.0
27	38.77832156604817	40.0	38.0	41.0	35.0	41.0
28	38.72296400630105	40.0	38.0	41.0	35.0	41.0
29	38.588142950991575	40.0	38.0	41.0	35.0	41.0
30	38.54925855632746	40.0	38.0	41.0	35.0	41.0
31	38.47314491716105	40.0	38.0	41.0	34.0	41.0
32	38.403304405693284	40.0	38.0	41.0	34.0	41.0
33	38.27300592798158	40.0	38.0	41.0	34.0	41.0
34	38.272627430662475	40.0	38.0	41.0	34.0	41.0
35	38.25280983844835	40.0	38.0	41.0	34.0	41.0
36	38.22939341926567	40.0	38.0	41.0	34.0	41.0
37	38.20305527999882	40.0	38.0	41.0	34.0	41.0
38	38.134942050354105	40.0	38.0	41.0	34.0	41.0
39	37.95106913858509	40.0	38.0	41.0	33.0	41.0
40	37.96368675003432	40.0	37.0	41.0	33.0	41.0
41	37.96694043088197	40.0	37.0	41.0	33.0	41.0
42	37.85401637621393	40.0	37.0	41.0	33.0	41.0
43	37.04710352599726	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	2.0
14	0.0
15	1.0
16	4.0
17	7.0
18	19.0
19	37.0
20	97.0
21	155.0
22	330.0
23	673.0
24	971.0
25	1633.0
26	2484.0
27	3706.0
28	5449.0
29	7733.0
30	11004.0
31	14635.0
32	19770.0
33	26774.0
34	38064.0
35	56279.0
36	88955.0
37	165784.0
38	383678.0
39	461063.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.780519642692326	19.25341403806225	12.897063465778958	26.069002853466472
2	18.8718142819138	20.8807973883685	34.805388002410595	25.442000327307106
3	20.316851895084888	22.292173559635433	30.282034795382646	27.108939749897036
4	15.519475936334889	16.131509203767287	33.92344271233661	34.42557214756121
5	15.717566541457478	35.05513418428011	33.698748709580094	15.528550564682323
6	34.42495166015284	34.232755685409785	15.806839167336923	15.535453487100453
7	28.68901093531496	30.028177884432665	21.359736106705217	19.923075073547146
8	26.456885044624677	33.39556304966459	20.341748952345792	19.805802953364942
9	25.943664396975436	14.868817327731366	20.492527392580058	38.69499088271314
10	17.23093533047547	26.95932472355347	33.52377126041934	22.28596868555172
11	35.17985215336277	21.669359323482578	21.7417236674839	21.40906485567075
12	21.359115619296848	25.39631694186576	29.214098404649313	24.03046903418808
13	30.460812729919667	20.02413696018565	24.296890815157575	25.2181594947371
14	22.839133210114877	21.264801533224386	24.929787971696467	30.96627728496427
15	25.27152141185705	27.638448192016035	22.401922269991132	24.688108126135784
16	24.93188211669972	26.394215816379162	24.09655094317964	24.57735112374148
17	23.406103579514298	26.568417656279447	25.700355771967775	24.32512299223848
18	23.411455283411502	25.60138803033253	26.598123490955235	24.389033195300737
19	24.218011353368354	25.784199133023968	26.79179312329317	23.2059963903145
20	24.454727299662064	25.361259403292774	26.60867177689755	23.575341520147614
21	24.451857545398347	25.683602611941748	25.702837721601263	24.161702121058646
22	24.66429692183953	25.449446176207562	25.610307536827865	24.27594936512504
23	24.55757308759964	25.435097404888975	25.674450422668265	24.33287908484312
24	24.90473579258347	25.312240898031423	25.54523391987491	24.237789389510194
25	24.66406423906139	25.123845408664643	25.80521814398255	24.406872208291418
26	24.33132786632219	25.351719409389062	25.824453253642066	24.49249947064668
27	24.06831876609874	25.52196564206098	25.788232301178382	24.621483290661896
28	24.05482316496666	25.49148419812473	26.043873113427424	24.409819523481183
29	23.940343238122125	25.810802530657895	26.167350107693345	24.081504123526635
30	23.939102263305383	25.994932169092127	25.993070706867012	24.07289486073548
31	24.255007915092502	25.51413198853029	26.041546285646035	24.189313810731175
32	23.7507067739386	25.538098314678635	26.280433937869045	24.430760973513717
33	23.93917982423143	25.318368211189096	26.38622704099638	24.3562249235831
34	24.11547580913497	24.972756724726192	26.22319397444678	24.68857349169206
35	23.900089117504027	25.066140079686093	26.513969886194854	24.519800916615022
36	23.875657425799403	25.03426253908101	26.41360604789077	24.676473987228817
37	24.052186093481083	24.026048061403436	26.803970188682463	25.117795656433017
38	23.4017601676557	24.451314618916022	27.693749132287138	24.45317608114114
39	23.232289544244242	24.188072835914433	28.138250799459247	24.44138682038208
40	22.88380830351762	23.656625370644274	28.91998737308124	24.539578952756862
41	22.448691508397133	23.859292070403605	28.81155719846833	24.880459222730934
42	22.38245447755348	23.61652637187827	28.968152708156076	25.03286644241218
43	21.79112997737548	23.66345073213636	29.174154527735404	25.37126476275276
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	57.0
2	95.0
3	240.0
4	385.0
5	385.0
6	522.5
7	660.0
8	653.0
9	646.0
10	976.5
11	1307.0
12	1307.0
13	2135.5
14	2964.0
15	4440.5
16	5917.0
17	6243.5
18	6570.0
19	6570.0
20	8420.0
21	10270.0
22	11434.0
23	12598.0
24	15239.0
25	17880.0
26	17880.0
27	20743.5
28	23607.0
29	28721.5
30	33836.0
31	37654.0
32	41472.0
33	41472.0
34	46082.0
35	50692.0
36	55153.5
37	59615.0
38	64455.5
39	69296.0
40	69296.0
41	74084.0
42	78872.0
43	78148.0
44	77424.0
45	80016.0
46	82608.0
47	82608.0
48	84297.5
49	85987.0
50	88709.5
51	91432.0
52	93774.5
53	96117.0
54	96117.0
55	91409.0
56	86701.0
57	82621.5
58	78542.0
59	72857.0
60	67172.0
61	67172.0
62	61905.0
63	56638.0
64	50717.5
65	44797.0
66	39269.0
67	33741.0
68	33741.0
69	29188.5
70	24636.0
71	21269.0
72	17902.0
73	14823.5
74	11745.0
75	11745.0
76	9527.5
77	7310.0
78	5846.0
79	4382.0
80	3523.5
81	2665.0
82	2665.0
83	2069.0
84	1473.0
85	1114.5
86	756.0
87	560.5
88	365.0
89	365.0
90	260.5
91	156.0
92	98.0
93	40.0
94	25.5
95	11.0
96	11.0
97	8.5
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1289309.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.44385282214263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.84364263584632	59.61313284920321
2	9.466981975525197	13.148474059564977
3	2.181330291254411	4.544399391070584
4	0.8439648224809551	2.344326756777327
5	0.41926162057892397	1.45575711367279
6	0.24142930352609948	1.005946861261131
7	0.16523891230193644	0.8032378694470624
8	0.11270345225288649	0.6261249560637463
9	0.08549473551366686	0.5343375447071183
>10	0.5453536567191499	7.171664392019014
>50	0.05356848826269184	2.635041169919075
>100	0.039353141834294086	4.988178051265627
>500	0.0012297735292563833	0.5590608421797728
>1k	4.4719037427504843E-4	0.5703181428485781
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2503	0.19413499789422084	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2104	0.16318818840169425	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1612	0.1250282127868494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.5512185209286524E-4	0.0
10	0.0	0.0	0.0	1.5512185209286524E-4	0.0
11	0.0	0.0	0.0	1.5512185209286524E-4	0.0
12	0.0	0.0	0.0	1.5512185209286524E-4	0.0
13	0.0	0.0	0.0	1.5512185209286524E-4	0.0
14	0.0	7.756092604643262E-5	0.0	1.5512185209286524E-4	0.0
15	0.0	7.756092604643262E-5	0.0	2.3268277813929788E-4	0.0
16	0.0	7.756092604643262E-5	0.0	3.102437041857305E-4	0.0
17	0.0	7.756092604643262E-5	0.0	3.878046302321631E-4	0.0
18	0.0	7.756092604643262E-5	0.0	5.429264823250283E-4	0.0
19	0.0	7.756092604643262E-5	0.0	5.429264823250283E-4	0.0
20	0.0	7.756092604643262E-5	0.0	6.20487408371461E-4	0.0
21	0.0	7.756092604643262E-5	0.0	6.20487408371461E-4	0.0
22	0.0	7.756092604643262E-5	0.0	7.756092604643262E-4	0.0
23	0.0	7.756092604643262E-5	0.0	0.0010858529646500567	0.0
24	0.0	7.756092604643262E-5	0.0	0.002249266855346546	0.0
25	0.0	7.756092604643262E-5	0.0	0.0035678025981359007	0.0
26	0.0	7.756092604643262E-5	0.0	0.00620487408371461	0.0
27	0.0	7.756092604643262E-5	0.0	0.025672666521369198	0.0
28	0.0	7.756092604643262E-5	0.0	0.07694043863806116	0.0
29	0.0	7.756092604643262E-5	0.0	0.13852381391892868	0.0
30	0.0	7.756092604643262E-5	0.0	0.22911497554116197	0.0
31	0.0	7.756092604643262E-5	0.0	0.47963676667113936	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	780	0.0	26.564104	1
CGCGCTT	40	0.0019313142	23.125	12
GCGGATA	50	0.007035337	18.499998	29
TATACCG	50	0.007035337	18.499998	5
TATGCCG	315	0.0	18.20635	33
CCGTCTT	330	0.0	17.378788	37
ATGCCGT	330	0.0	17.378788	34
GCCGTCT	330	0.0	17.378788	36
TTATACT	160	3.45608E-11	17.34375	4
GTATCAA	1210	0.0	16.971075	2
CGTATGC	340	0.0	16.867647	31
CTCGTAT	355	0.0	16.15493	29
TCGTATG	360	0.0	15.930555	30
ACCGAAC	70	0.0025927231	15.857142	3
GTTGTCG	75	0.004104765	14.800001	21
TGCCGTC	400	0.0	14.799999	35
ATGCGTT	115	2.2101463E-5	14.478261	36
CGTTTAG	115	2.2101463E-5	14.478261	26
TCTCGTA	405	0.0	14.160494	28
CTTATAC	915	0.0	14.153006	37
>>END_MODULE