FastQCFastQC Report
Fri 10 Feb 2017
ERR1632123.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632123.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9360
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT890.9508547008547008TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT480.5128205128205128No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC400.4273504273504274No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC390.4166666666666667No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG200.2136752136752137No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG180.19230769230769232No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG120.1282051282051282No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC120.1282051282051282RNA PCR Primer, Index 3 (95% over 22bp)
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG110.11752136752136752No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA100.10683760683760685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTC955.9410435E-515.57894837
Produced by FastQC (version 0.11.5)