##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632123.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9360
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.570299145299145	34.0	31.0	34.0	31.0	34.0
2	32.75213675213675	34.0	31.0	34.0	31.0	34.0
3	32.74797008547009	34.0	31.0	34.0	31.0	34.0
4	36.257371794871794	37.0	37.0	37.0	35.0	37.0
5	36.1508547008547	37.0	35.0	37.0	35.0	37.0
6	36.17820512820513	37.0	36.0	37.0	35.0	37.0
7	36.175534188034185	37.0	36.0	37.0	35.0	37.0
8	36.16602564102564	37.0	35.0	37.0	35.0	37.0
9	37.93803418803419	39.0	38.0	39.0	35.0	39.0
10	37.83728632478633	39.0	38.0	39.0	35.0	39.0
11	37.94049145299145	39.0	38.0	39.0	35.0	39.0
12	37.896794871794874	39.0	38.0	39.0	35.0	39.0
13	37.94540598290598	39.0	38.0	39.0	35.0	39.0
14	39.29497863247863	40.0	39.0	41.0	36.0	41.0
15	39.303525641025644	40.0	39.0	41.0	36.0	41.0
16	39.22104700854701	40.0	39.0	41.0	36.0	41.0
17	39.218910256410254	40.0	39.0	41.0	36.0	41.0
18	39.230235042735046	40.0	39.0	41.0	36.0	41.0
19	39.30854700854701	40.0	39.0	41.0	36.0	41.0
20	39.28867521367521	40.0	39.0	41.0	36.0	41.0
21	39.28301282051282	40.0	39.0	41.0	36.0	41.0
22	39.211858974358975	40.0	39.0	41.0	36.0	41.0
23	39.24551282051282	40.0	39.0	41.0	36.0	41.0
24	39.212606837606835	40.0	39.0	41.0	36.0	41.0
25	39.10673076923077	40.0	39.0	41.0	36.0	41.0
26	39.05523504273504	40.0	39.0	41.0	36.0	41.0
27	38.94305555555555	40.0	38.0	41.0	35.0	41.0
28	38.95747863247863	40.0	38.0	41.0	35.0	41.0
29	38.78589743589744	40.0	38.0	41.0	35.0	41.0
30	38.75235042735043	40.0	38.0	41.0	35.0	41.0
31	38.61816239316239	40.0	38.0	41.0	35.0	41.0
32	38.58568376068376	40.0	38.0	41.0	35.0	41.0
33	38.4258547008547	40.0	38.0	41.0	34.0	41.0
34	38.44220085470086	40.0	38.0	41.0	34.0	41.0
35	38.415705128205126	40.0	38.0	41.0	34.0	41.0
36	38.435790598290595	40.0	38.0	41.0	34.0	41.0
37	38.413568376068376	40.0	38.0	41.0	34.0	41.0
38	38.405662393162395	40.0	38.0	41.0	34.0	41.0
39	38.19465811965812	40.0	38.0	41.0	34.0	41.0
40	38.17051282051282	40.0	38.0	41.0	34.0	41.0
41	38.275747863247865	40.0	38.0	41.0	34.0	41.0
42	38.15630341880342	40.0	38.0	41.0	34.0	41.0
43	37.265491452991455	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	4.0
22	1.0
23	0.0
24	3.0
25	13.0
26	9.0
27	23.0
28	23.0
29	42.0
30	53.0
31	105.0
32	125.0
33	194.0
34	268.0
35	370.0
36	617.0
37	1093.0
38	2587.0
39	3830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.19658119658119	18.098290598290596	13.226495726495727	27.47863247863248
2	19.444444444444446	20.30982905982906	32.40384615384615	27.841880341880344
3	20.053418803418804	21.207264957264957	30.480769230769234	28.25854700854701
4	15.331196581196583	15.384615384615385	31.35683760683761	37.927350427350426
5	16.35683760683761	33.47222222222222	33.75	16.42094017094017
6	37.67094017094017	32.02991452991453	14.476495726495727	15.822649572649572
7	30.064102564102562	28.952991452991455	19.14529914529915	21.837606837606838
8	29.412393162393162	31.164529914529915	19.797008547008545	19.626068376068375
9	26.153846153846157	13.397435897435898	17.564102564102562	42.88461538461538
10	18.108974358974358	25.405982905982903	31.185897435897438	25.299145299145298
11	36.93376068376069	19.155982905982906	20.993589743589745	22.916666666666664
12	23.77136752136752	23.824786324786324	26.07905982905983	26.324786324786327
13	33.215811965811966	17.64957264957265	21.55982905982906	27.574786324786327
14	24.935897435897438	19.294871794871796	21.8482905982906	33.92094017094017
15	26.645299145299145	26.517094017094017	19.572649572649574	27.264957264957264
16	28.4508547008547	23.386752136752136	20.833333333333336	27.329059829059826
17	25.833333333333336	25.91880341880342	22.136752136752136	26.111111111111114
18	24.925213675213676	23.696581196581196	23.792735042735043	27.58547008547009
19	27.521367521367523	23.11965811965812	24.326923076923077	25.03205128205128
20	27.735042735042736	21.955128205128204	24.337606837606838	25.972222222222225
21	28.183760683760685	23.728632478632477	20.97222222222222	27.115384615384613
22	27.4465811965812	23.910256410256412	22.275641025641026	26.36752136752137
23	27.521367521367523	23.01282051282051	21.74145299145299	27.724358974358974
24	29.423076923076923	22.232905982905983	22.30769230769231	26.036324786324787
25	27.393162393162395	23.557692307692307	22.788461538461537	26.26068376068376
26	27.852564102564102	23.835470085470085	21.69871794871795	26.613247863247864
27	26.623931623931625	24.02777777777778	23.194444444444446	26.153846153846157
28	26.431623931623932	23.26923076923077	23.194444444444446	27.10470085470085
29	27.07264957264957	23.247863247863247	23.226495726495727	26.452991452991455
30	25.940170940170944	23.621794871794872	24.177350427350426	26.26068376068376
31	26.506410256410255	23.846153846153847	23.482905982905983	26.164529914529915
32	26.655982905982906	23.06623931623932	23.30128205128205	26.976495726495724
33	24.09188034188034	23.86752136752137	24.86111111111111	27.17948717948718
34	26.613247863247864	23.77136752136752	23.42948717948718	26.185897435897438
35	25.91880341880342	22.318376068376068	25.865384615384617	25.8974358974359
36	26.655982905982906	23.034188034188034	23.00213675213675	27.307692307692307
37	25.213675213675213	21.944444444444443	24.615384615384617	28.226495726495727
38	24.62606837606838	21.73076923076923	26.26068376068376	27.382478632478634
39	24.775641025641026	20.705128205128208	27.21153846153846	27.307692307692307
40	23.92094017094017	19.786324786324787	29.583333333333332	26.70940170940171
41	24.444444444444443	20.096153846153847	28.333333333333332	27.126068376068375
42	22.77777777777778	19.732905982905983	29.65811965811966	27.831196581196583
43	21.613247863247864	19.96794871794872	30.02136752136752	28.397435897435898
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	1.5
9	2.0
10	3.0
11	4.0
12	4.0
13	4.5
14	5.0
15	5.0
16	5.0
17	7.0
18	9.0
19	9.0
20	13.0
21	17.0
22	20.5
23	24.0
24	28.5
25	33.0
26	33.0
27	37.5
28	42.0
29	62.0
30	82.0
31	103.0
32	124.0
33	124.0
34	178.5
35	233.0
36	244.5
37	256.0
38	298.0
39	340.0
40	340.0
41	401.0
42	462.0
43	489.5
44	517.0
45	552.0
46	587.0
47	587.0
48	640.5
49	694.0
50	740.0
51	786.0
52	806.0
53	826.0
54	826.0
55	910.5
56	995.0
57	871.0
58	747.0
59	668.5
60	590.0
61	590.0
62	571.0
63	552.0
64	506.5
65	461.0
66	389.5
67	318.0
68	318.0
69	272.5
70	227.0
71	184.5
72	142.0
73	125.5
74	109.0
75	109.0
76	90.5
77	72.0
78	56.0
79	40.0
80	35.0
81	30.0
82	30.0
83	22.5
84	15.0
85	10.5
86	6.0
87	5.5
88	5.0
89	5.0
90	3.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9360.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.69871794871794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.3765583129442	88.37606837606837
2	2.365140393801701	4.337606837606837
3	0.6174997087265525	1.6987179487179487
4	0.25632063381102177	0.9401709401709402
5	0.13981125480601186	0.641025641025641
6	0.04660375160200396	0.2564102564102564
7	0.04660375160200396	0.2991452991452992
8	0.034952813701502966	0.2564102564102564
9	0.0	0.0
>10	0.10485844110450893	2.2435897435897436
>50	0.01165093790050099	0.9508547008547008
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	89	0.9508547008547008	TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	48	0.5128205128205128	No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	40	0.4273504273504274	No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	39	0.4166666666666667	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	20	0.2136752136752137	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	18	0.19230769230769232	No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG	12	0.1282051282051282	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	12	0.1282051282051282	RNA PCR Primer, Index 3 (95% over 22bp)
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG	11	0.11752136752136752	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	10	0.10683760683760685	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.010683760683760684	0.0
25	0.0	0.0	0.0	0.010683760683760684	0.0
26	0.0	0.0	0.0	0.010683760683760684	0.0
27	0.0	0.0	0.0	0.021367521367521368	0.0
28	0.0	0.0	0.0	0.11752136752136752	0.0
29	0.0	0.0	0.0	0.19230769230769232	0.0
30	0.0	0.0	0.0	0.29914529914529914	0.0
31	0.0	0.0	0.0	0.5982905982905983	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCTC	95	5.9410435E-5	15.578948	37
>>END_MODULE