FastQCFastQC Report
Fri 10 Feb 2017
ERR1632122.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632122.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365144
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41010.3004078690599673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28210.20664486676863392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26180.1917746406239928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.018.6637151
TATTGTC1800.017.4722215
GTATACT650.001580139317.0769234
AATAACG650.001580139317.0769232
CGAATTA1107.810231E-716.81818215
GGACCGT1107.810231E-716.8181826
CTTATAC11400.016.55263137
TATATAC1059.345591E-615.8571422
TAACGAT700.00259282815.85714223
TCTTATA18750.015.78666837
AACCGAC855.3650676E-415.23529434
TGTCCGA3800.015.09210612
TAGACGT750.00410493114.84
TTAGACC750.00410493114.84
CTAGGTA750.00410493114.84
ATAGGGG1252.9595449E-614.7999993
GCTTAGG3300.014.5757591
TTATACT1152.2103335E-514.4782614
GTATATA908.2754187E-414.3888881
CCTATAC1551.2104647E-714.3225813
Produced by FastQC (version 0.11.5)